Probing Methionine Uptake in Live Cells by Deuterium Labeling and Stimulated Raman Scattering

The Journal of Physical Chemistry B, Journal Year: 2022, Volume and Issue: 126(8), P. 1633 - 1639

Published: Feb. 23, 2022

The small biomolecule methionine (Met) is a fundamental amino acid required for vast range of biological processes such as protein synthesis, cancer metabolism, and epigenetics. However, it still difficult to visualize the subcellular distribution biomolecules including Met in minimally invasive manner. Here, we demonstrate stimulated Raman scattering (SRS) imaging cellular uptake deuterated (d8-Met) live HeLa cells by way comparison previously used alkyne-labeled analogue─homopropargylglycine (Hpg). We show that solutions d8-Met Hpg have similar SRS signal intensities. Furthermore, careful image analysis with background subtraction, succeed much greater intensity than Hpg, possibly reflecting increased kinetics compared Hpg. anticipate other will be useful investigating metabolic resolution.

Language: Английский

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Association of serum s-adenosylmethionine, s-adenosylhomocysteine, and their ratio with the risk of dementia and death in a community

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: July 20, 2022

Abstract We examined the association of serum s-adenosylmethionine (SAM), s-adenosylhomocysteine (SAH) (methionine metabolites), and their ratio on risk dementia death in a community-dwelling population older Japanese individuals. 1371 residents Hisayama, Japan, aged 65 years or without dementia, were followed for median 10.2 (2007–2017). divided SAM, SAH, SAM/SAH into quartiles. Cox proportional hazards models used to estimate hazard ratios (HRs) 95% confidence intervals (CIs) levels composite outcome all-cause death, each outcome. During follow-up, 635 participants developed and/or died, which 379 394 deaths occurred. The multivariable-adjusted HRs decreased significantly with increasing SAM ( P trend = 0.01), while they increased higher SAH 0.03). Higher associated lower 0.002), as well Our findings suggest that balance methionine metabolites may closely associate death.

Language: Английский

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Methyl Donor Micronutrients: A Potential Dietary Epigenetic Target in Systemic Lupus Erythematosus Patients

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3171 - 3171

Published: Feb. 6, 2023

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by an aberrant immune response and persistent inflammation. Its pathogenesis remains unknown; however, complex interaction between environmental, genetic, epigenetic factors has been suggested to cause onset. Several studies have demonstrated that alterations, such as DNA hypomethylation, miRNA overexpression, altered histone acetylation, may contribute SLE onset the disease's clinical manifestations. Epigenetic changes, especially methylation patterns, are modifiable susceptible environmental diet. It well known methyl donor nutrients, folate, methionine, choline, some B vitamins, play relevant role in participating donors or coenzymes one-carbon metabolism. Based on this knowledge, critical literature review aimed integrate evidence animal models humans regarding of nutrients homeostasis their impact system regulation suggest potential diet could serve adjuvant therapy SLE.

Language: Английский

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Association of Long-term Exposure to Air Pollution and Dementia Risk

Neurology, Journal Year: 2023, Volume and Issue: 101(12)

Published: July 13, 2023

Growing evidence links air pollution with dementia risk, but the biological mechanisms are largely unknown. We investigated role played by homocysteine (tHcy) and methionine in this association explored whether could be explained cardiovascular diseases (CVDs).Data were extracted from ongoing Swedish National study on Aging Care Kungsholmen (SNAC-K), a longitudinal population-based study. At baseline, 2,512 dementia-free participants examined up to 2013 (mean follow-up: 5.18 ± 2.96 years). Two pollutants (particulate matter ≤2.5 μm [PM2.5] nitrogen oxides [NOx]) assessed yearly 1990 until using dispersion models at residential addresses. The hazard ratio of over levels was estimated Cox adjusted for age, sex, education, smoking, socioeconomic status, physical activity, retirement creatinine, year assessment, use supplements. total effect decomposed into 4 pathways involving tHcy/methionine: (1) direct effect; (2) indirect (mediation); (3) due interaction; (4) both mediation interaction. To test independent CVDs (ischemic heart disease, atrial fibrillation, failure, stroke), we repeated analyses excluding those individuals who developed CVDs.The mean age 73.4 years (SD: 10.4), 62.1% female individuals. During an average period 5 (mean: 5.18; SD: years), 376 cases incident identified. There 70% increased per unit increase PM2.5 during before baseline (hazard [HR]: 1.71; 95% CI 1.33-2.09). Overall, 50% (51.6%; 9.0-94.1) tHcy (6.6%; 1.6-11.6) and/or interaction (47.8%; 4.9-91.7) 48.4% (p = 0.03) dementia. High reduced linked 31% (HR: 0.69; 0.56-0.85) 24.8% attributable 25.9% 0.001) PM2.5. No found through methionine. Attenuated results obtained NOx. Findings attenuated after CVDs, while remained similar methionine.High enhanced risk attributed pollution, high concentrations risk. impact conditions partly explains association. Alternative other than may play between

Language: Английский

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The Role of Organokines in Obesity and Type 2 Diabetes and Their Functions as Molecular Transducers of Nutrition and Exercise

Metabolites, Journal Year: 2023, Volume and Issue: 13(9), P. 979 - 979

Published: Aug. 29, 2023

Maintaining systemic homeostasis requires the coordination of different organs and tissues in body. Our bodies rely on complex inter-organ communications to adapt perturbations or changes metabolic homeostasis. Consequently, liver, muscle, adipose produce secrete specific organokines such as hepatokines, myokines, adipokines response nutritional environmental stimuli. Emerging evidence suggests that dysregulation interplay between is associated with pathophysiology obesity type 2 diabetes (T2D). Strategies aimed at remodeling may be effective therapeutic interventions. Diet modification exercise have been established first-line intervention prevent treat diseases. This review summarizes current knowledge secreted by T2D. Additionally, we highlighted effects diet/nutrition Specifically, investigated ameliorative caloric restriction, selective nutrients including ω3 PUFAs, selenium, vitamins, metabolites acute/chronic Finally, this study dissected underlying molecular mechanisms which nutrition regulate expression secretion tissues.

Language: Английский

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