Efficacy of cleaning, disinfection and sterilization modalities for addressing infectious drug-resistant fungi: A review

Journal of Applied Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract This is a timely and important review that focuses on the appropriateness of established cleaning, disinfection sterilization methods to safely effectively address infectious fungal drug-resistant pathogens can potentially contaminate reusable medical devices used in healthcare environment order mitigate risk patient infection. The release World Health Organisation (WHO) priority pathogen list (FPPL) 2022 highlighted public health crisis antimicrobial resistance (AMR) clinically relevant species. Contamination with (including those FPPL) are rare events more likely occur due cross-transmission arising from lapses hand-hygiene practices. Established decontaminate single use devices; however, there assumptions destined for semi-critical appropriately cleaned do not harbour biofilms may undermine ability decontamination these type healthcare. International standards dictate manufacturer's instructions must provide appropriate guidance facilities meet safe reprocessing expectations includes addressing drug resistant pathogens. Increased environmental monitoring vigilance surrounding advised including adherence hand hygiene/aseptic practices cleaning encompassing simplification device features ‘ease-of-reach’. There need promote integrated multi-actor hub approach sophisticated challenges future artificial intelligence machine learning improved diagnostics, monitoring/surveillance (such as wastewater-based epidemiology), sterility assurance design. knowledge gap occurrence potential persistence harboured biofilms, particularly ascertaining efficacy high-level devices.

Language: Английский

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Advances in microfluidics for detection of infectious diseases

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 345 - 365

Published: Jan. 1, 2025

Language: Английский

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Transforming early microbial detection: Investigating innovative biosensors for emerging infectious diseases

Advances in Biomarker Sciences and Technology, Journal Year: 2024, Volume and Issue: 6, P. 59 - 71

Published: Jan. 1, 2024

The recent global pandemic has highlighted an increase in the prevalence of communicable diseases caused by pathogens. swift transmission these within a short timeframe presents substantial risk to public health worldwide. inefficiency traditional diagnostic instruments, which need time-consuming and complex process laboratory, is significant obstacle medical care. Currently, there high for advancement early detection order rapidly diagnose infectious provide on-site results. This crucial prompt intervention improve treatment outcomes. also provides rapid testing high-quality microbiological detection, comparable laboratory standards, matter minutes. Prompt diagnosis subsequent optimization aid controlling spread diseases. ongoing techniques methods are used advancements through biosensors. review examines integration diagnostics with biosensors, specifically relation emerging re-emerging diseases, challenges, future perspective.

Language: Английский

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Hospital Acquired Sepsis, Disease Prevalence, and Recent Advances in Sepsis Mitigation

Pathogens, Journal Year: 2024, Volume and Issue: 13(6), P. 461 - 461

Published: May 30, 2024

Sepsis is a life-threatening organ dysfunction caused by dysregulated host response to infection, commonly associated with nosocomial transmission. Gram-negative bacterial species are particularly problematic due the release of lipopolysaccharide toxins upon cell death. The toxin E. coli has greater immunogenic potential than that other bacteria. resultant dysregulation immune system failure and mortality, pregnant women, ICU patients, neonates being vulnerable. Additionally, sepsis recovery patients have an increased risk re-hospitalisation, chronic illness, co-morbidities, damage/failure, reduced life expectancy. emergence increasing prevalence antimicrobial resistance in fungal impacted treatment leading mortality rates. Multidrug resistant pathogens including vancomycin-resistant Enterococcus, beta lactam-resistant Klebsiella, carbapenem-resistant Acinetobacter mortality. To improve prognosis predominantly high-risk neonates, advances must be made early diagnosis, triage, control sepsis. identification suitable biomarkers biomarker combinations, coupled machine learning artificial intelligence, show promise detection protocols. Rapid diagnosis essential inform on clinical treatment, especially infectious agents. This timely review aims discuss prevalence, aetiology, recent towards disease mitigation control.

Language: Английский

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Rapid Microfluidic Ion-Exchange Optode System for Point-of-Care Determination of Sodium Concentration in Serum

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 104 - 104

Published: Feb. 12, 2025

A microfluidic system for detecting sodium ions (Na+) has been developed, incorporating a micro finger-pump chip and micro-spectrometer platform to measure Na+ concentration in human serum. small volume (10 μL) of serum sample is introduced into the microchip reacted with preloaded reagent mixture through two-step actuation process. The resulting purple complex directed detection area analyzed using at wavelengths 555 666 nm. then inversely derived from measured A555/A666 absorbance ratio self-written software installed on Raspberry Pi. entire process completed just 3 min, offering significant advantage meeting clinical needs compared traditional reporting turnaround time several hours medical institutions. experimental results indicate linear relationship between within range 1-200 mM, correlation coefficient R2 = 0.9989. Additionally, 60 samples collected chronic kidney disease (CKD) patients showed strong agreement those obtained conventional indirect ion-selective electrode (ISE) method, achieving 0.9885 an average recovery rate 99.4%. In summary, proposed provides practical, affordable, rapid alternative methods, making it highly promising point-of-care (POC) testing applications.

Language: Английский

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Reducing hepatitis C diagnostic disparities with a fully automated deep learning–enabled microfluidic system for HCV antigen detection

Science Advances, Journal Year: 2025, Volume and Issue: 11(12)

Published: March 19, 2025

Viral hepatitis remains a major global health issue, with chronic B (HBV) and C (HCV) causing approximately 1 million deaths annually, primarily due to liver cancer cirrhosis. More than 1.5 people contract HCV each year, disproportionately affecting vulnerable populations, including American Indians Alaska Natives (AI/AN). While direct-acting antivirals (DAAs) are highly effective, timely accurate diagnosis challenge, particularly in resource-limited settings. The current two-step testing process is costly time-intensive, often leading patient loss before treatment. Point-of-care (POC) antigen (Ag) offers promising alternative, but no FDA-approved test meets the required sensitivity specificity. To address this, we developed fully automated, smartphone-based POC Ag assay using platinum nanoparticles, deep learning image processing, microfluidics. With an overall accuracy of 94.59%, this cost-effective, portable device has potential reduce HCV-related disparities, among AI/AN improving accessibility equity care.

Language: Английский

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A microfluidic system for rapid enrichment and sensitive detection of E. coli based on bilayer membrane and high flux droplets

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128005 - 128005

Published: March 1, 2025

Language: Английский

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Neonatal Infectious Disease: A Major Contributor to Infant Mortality Requiring Advances in Point-of-Care Diagnosis

Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 877 - 877

Published: Sept. 13, 2024

Neonatal infectious disease continues to result in high rates of infant morbidity and mortality. Early- late-onset represent difficult detect treat illnesses, particularly when antimicrobial resistant pathogens are present. Newborns immunodeficient at increased risk vertical horizontal infection, with preterm infants increasingly susceptible. Additional factors associated infection include prolonged use a central catheter and/or ventilation, congenital abnormalities, admittance intensive care units, the broad-spectrum antibiotics. There is increasing recognition importance host microbiome dysbiosis on neonatal disease, including necrotising enterocolitis sepsis patients. Current diagnostic methods rely blood culture, which unreliable, time consuming, can false negatives. lack accurate reliable tools available for early detection infants; therefore, efficient triage treatment remains challenging. The application biomarkers, machine learning, artificial intelligence, biosensors, microfluidics technology, may offer improved methodologies. Point-of-care devices, such methodologies, provide fast, reliable, aids This review will discuss as impacted by resistance highlight novel point-of-care options.

Language: Английский

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Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 545 - 545

Published: Nov. 10, 2024

Rheumatoid arthritis (RA) is a chronic autoimmune disorder that causes extensive damage to multiple organs and tissues has no known cure. This study introduces microfluidic detection platform combines reaction chip with micro-spectrometer accurately detect the anti-cyclic citrullinated peptide antibody (anti-CCP Ab) biomarker, commonly associated arthritis. The surface of functionalized using streptavidin enable subsequent immobilization biotinylated-labeled cyclic (biotin-CCP) molecules through streptavidin-biotin reaction. modified then exposed anti-CCP Ab, second conjugated horseradish peroxidase (HRP) (2nd Ab-HRP), 3,3',5,5'-tetramethylbenzidine (TMB), stop solution. Finally, concentration Ab biomarker determined by analyzing optical density (OD) colorimetric product at 450 nm micro-spectrometer. demonstrated strong correlation (R

Language: Английский

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SERS-Based Optical Nanobiosensors for the Detection of Alzheimer’s Disease

Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 880 - 880

Published: Sept. 11, 2023

Alzheimer’s disease (AD) is a leading cause of dementia, impacting millions worldwide. However, its complex neuropathologic features and heterogeneous pathophysiology present significant challenges for diagnosis treatment. To address the urgent need early AD diagnosis, this review focuses on surface-enhanced Raman scattering (SERS)-based biosensors, leveraging excellent optical properties nanomaterials to enhance detection performance. These highly sensitive noninvasive biosensors offer opportunities biomarker-driven clinical diagnostics precision medicine. The highlights various types SERS-based targeting biomarkers, discussing their potential applications contributions diagnosis. Specific details about targeted biomarkers are provided. Furthermore, future research directions improving marker using SERS sensors outlined.

Language: Английский

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