Recent advances in 3D bioprinted neural models: A systematic review on the applications to drug discovery

Advanced Drug Delivery Reviews, Journal Year: 2025, Volume and Issue: 218, P. 115524 - 115524

Published: Feb. 1, 2025

The design of neural tissue models with architectural and biochemical relevance to native tissues opens the way for fundamental study development therapies many disorders limited treatment options. Here, we systematically review most recent literature on 3D bioprinted models, including their potential use in drug screening. Neural that model central nervous system (CNS) from relevant are reviewed comprehensive summaries each study, discussion types, bioinks additives, cell types used, construct shapes culture time, characterization methods used. In this review, accentuate lack standardization among analyze functionality (including chemical, metabolic other pathways) mechanical constructs, discuss as a critical area future exploration. These gaps must be addressed technology applied effective screening applications, despite its enormous rapid efficient biomimetic, printed is promising evaluation vivo multiple levels should sought adequately compare performance develop viable options neurodegenerative diseases, or conditions affect CNS.

Language: Английский

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Microfluidics and Organ-on-a-Chip for Disease Modeling and Drug Screening

Biosensors, Journal Year: 2024, Volume and Issue: 14(2), P. 86 - 86

Published: Feb. 4, 2024

The convergence of microfluidics and organ-on-a-chip (OoC) technologies has revolutionized our ability to create advanced in vitro models that recapitulate complex physiological processes [...]

Language: Английский

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From Molecules to Mind: The Critical Role of Chitosan, Collagen, Alginate, and Other Biopolymers in Neuroprotection and Neurodegeneration

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1017 - 1017

Published: Feb. 22, 2025

Neurodegenerative disorders present significant therapeutic challenges, particularly due to the complex nature of drug delivery central nervous system. This review investigates applications various biopolymers in neuroprotection and their potential role treating neurodegeneration. We a critical analysis natural synthetic biopolymers, focusing primarily on chitosan, fish collagen/gelatin, alginate as key agents. The examines fundamental mechanisms brain development neurodegeneration, establishing framework for understanding how these interact with neural tissues. By analyzing recent experimental studies, we evaluate effectiveness different biopolymer-based systems crossing blood-brain barrier subsequent neuroprotective effects. Additionally, promising materials, including lignin, poly lactic-co-glycolic acid, glucose-modified bovine serum albumin/procyanidin complexes, are briefly explored provide comprehensive overview current developments field. Our reveals that approaches offer unique advantages both delivery, potentially opening new avenues neurodegenerative conditions. synthesizes knowledge identifies directions future research strategies.

Language: Английский

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Construction of Multicellular Neural Tissue Using Three-Dimensional Printing Technology: Cell Interaction

Tissue Engineering Part B Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

The study of the human nervous system remains challenging due to its inherent complexity and difficulty in obtaining original samples. Three-dimensional (3D) bioprinting is a rapidly evolving technology field tissue engineering that has made significant contributions several disciplines, including neuroscience. In order more accurately reflect intricate multicellular milieu vivo environment, an increasing number studies have commenced experimentation with coprinting diverse cell types. This article provides overview technical details application 3D multiple types neuroscience, focusing on challenges research conducted based printing. review discusses interactions systems, stem applications, construction brain-like organoids, establishment disease models, potential for integrating other culture techniques.

Language: Английский

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The rise of 3D bioprinting advancements in modeling neurodegenerative diseases

Ibrain, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Neurodegenerative diseases (NDs) are disorders that drastically alter the physiological functioning of neurons in brain. These processes often accompanied by abnormal protein aggregates physical and chemical properties brain tissue peripheral nerves. The causes NDs complex, involving genetic factors, neuroinflammation, oxidative stress, environmental influences, lifestyle, while symptoms progression vary significantly based on mechanisms cell death. Currently, no definitive treatment exists for NDs, as underlying degenerative remain poorly understood. Existing therapies focus symptom alleviation but insufficient to halt or prevent disease progression. This highlights urgent need strategies mimic pathophysiology facilitating deeper insights development effective treatments. Conventional vitro vivo models attempt replicate fail capture complexity nervous its interactions. In this context, 3D microfluidic bioprinting emerges a transformative technology. By enabling precise deposition cells biomaterials, it allows creation with high degree structural functional complexity. advancements provide valuable platform faithfully modeling bridging critical gaps our understanding, paving way toward innovative therapeutic approaches.

Language: Английский

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Organ‐On‐a‐Chip devices: Technology Progress and Challenges

ChemBioChem, Journal Year: 2024, Volume and Issue: 25(23)

Published: Aug. 26, 2024

Abstract Organ‐On‐a‐Chip (OOC) is a multichannel 3D‐microfluidic cell‐culture system incorporated in chip that simulates the behavior of an organ. This technology relies on multidisciplinary science benefits from and contributes progress many fields including microbiology, microfluidics, biomaterials, bioengineering. review article summarizes achievements various organ‐on‐chip technologies. It highlights significant advantages this terms reducing animal testing providing personalized medical responses. In addition, paper demonstrates how OOC becoming promising powerful tool pharmaceutical research to combat diseases. predicts not only effects drugs target organs but also, using body‐on‐a‐chip systems, it may provide insights into side drug delivery other organs. Likewise, models used for construction organ‐on‐a‐chip devices are investigated along with design materials microfluidic devices. For each OOC, integrated monitoring within chips (e. g., sensors biosensors) discussed. We also discuss evolution FDA regulations potential near future integrating OOCs protocols support reduce need failure rates preclinical clinical studies.

Language: Английский

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Long-term organotypic brain slices cultured on collagen-based microcontact prints: A perspective for a brain-on-a-chip

Journal of Neuroscience Methods, Journal Year: 2023, Volume and Issue: 399, P. 109979 - 109979

Published: Sept. 30, 2023

Organotypic brain slices are three-dimensional 150 µm–thick sections of a postnatal day 10 mouse and can be cultured for several weeks in vitro. In such the complex cellular connections preserved with high viability. These connected to collagen-loaded microcontact prints develop simple brain-on-a-chip model. Using printing technique, many peptides or proteins printed onto semipermeable membrane linked slices. On these prints, brain-derived nerve fibers grow out, microglia get activated migrate also new vessels formed. Such model may allow drugs diagnostic method neurodegenerative diseases.

Language: Английский

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Tailoring epilepsy treatment: personalized micro-physiological systems illuminate individual drug responses

Annals of Medicine and Surgery, Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Introduction: Approximately 50 million people worldwide have epilepsy, with many not achieving seizure freedom. Organ-on-chip technology, which mimics organ-level physiology, could revolutionize drug development for epilepsy by replacing animal models in preclinical studies. Our goal is to determine if customized micro-physiological systems can lead tailored treatments epileptic patients. Materials and methods: A comprehensive literature search was conducted utilizing various databases, including PubMed, Ebscohost, Medline, the National Library of Medicine, using a predetermined strategy. We focused on articles that addressed role personalized individual responses discussed different types diagnosis, current treatment options. Additionally, explored components design considerations were reviewed identify challenges opportunities challenging cases. Results: The system offers more accurate cost-effective alternative traditional assessing effects, toxicities, disease mechanisms. Nevertheless, designing patient-specific presents critical considerations, integration analytical biosensors patient-derived cells, while addressing regulatory, material, biological complexities. Material selection, standardization, vascular systems, cost efficiency, real-time monitoring, ethical are also crucial successful use this technology development. Conclusion: future organ-on-chip holds great promise, potential integrate artificial intelligence machine learning

Language: Английский

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Microfluidics for brain endothelial cell-astrocyte interactions

Organs-on-a-Chip, Journal Year: 2023, Volume and Issue: 5, P. 100033 - 100033

Published: Dec. 1, 2023

With the approval of Food and Drug (FDA) Modernization Act 2.0, pharmaceutical industry is poised to expand its research components with a plethora alternative models, including organ-on-microfluidic chips in pharma biotechnology, resulting personalized approach. Microfluidics opens new possibilities for study cell biology, especially better understanding cell-cell interactions pathophysiology neurodegenerative diseases vitro, use these models assess efficacy novel therapies promising. These thumb-sized organ-on-a-chip systems have potential reduce animal testing replace simple 2D culture that do not succeed resemble complex physiology tissues organs. Restoring critical aspects endothelial-brain immune communication biomimetic system using microfluidics may accelerate process central nervous (CNS) drug discovery improve our mechanisms multiple diseases. In addition, organ-on-chip technologies can be used optimize targets toxicity real-time, which significantly help minimize requirements, as authorized by recent FDA Act. This Review initially summarizes fundamental advantages microfluidic creating compartmentalized three-dimensional architectures neural tissue cells such neurons, glial cells, endothelial their ability recapitulate spatiotemporal biophysicochemical gradients mechanical microenvironments. Then, brain cell-astroglia-on-a-chip focus on Alzheimer's disease, Parkinson's Huntington's disease amyotrophic lateral sclerosis are introduced. Finally, current limitations devices strategies overcome them discussed.

Language: Английский

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Semiconductors in the Digital Age: Evolution, Challenges, and Geopolitical Implications

Revista Científica Multidisciplinar Núcleo do Conhecimento, Journal Year: 2023, Volume and Issue: unknown, P. 133 - 150

Published: Dec. 19, 2023

Semiconductor Industry, the Foundation of Contemporary Technological Revolution, has been continuously evolving from early transistors to today’s advanced microchips. Historically dominated by powers such as United States, last decade witnessed rise Asia, especially China, a vital center for production and research, reshaping geopolitical balance sector. Driven growing demand electronic devices digitization, industry faces challenges recent chip shortage, exacerbated COVID-19 pandemic. However, emerging technologies like tunnel gate transistors, graphene, resistive memory promise further revolutionize field. This narrative review provides comprehensive analysis evolution semiconductors, highlighting technological innovations, current challenges, implications, with special focus on intersection between economy technology.

Language: Английский

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