Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Dec. 2, 2024
To
investigate
the
clinical
application
value
of
microfluidic-based
in
vitro
diagnostic
(IVD)
technology
pathogenic
detection
respiratory
tract
infections.
A
total
300
samples,
including
blood,
bronchoalveolar
lavage
fluid,
and
pleural
effusion,
were
collected
from
patients
with
The
samples
randomly
divided
into
three
groups:
A,
B,
C,
100
cases
each
group.
Group
used
traditional
microbiological
methods,
B
metagenomic
next-generation
sequencing
(mNGS)
technology,
C
both
IVD
methods
to
detect
microorganisms
samples.
positive
rate,
time,
cost
compared
among
groups.
performance
group
was
using
Receiver
Operating
Characteristic
(ROC)
curve.
Traditional
identified
38
(38%),
45
pathogens;
mNGS
95
(95%),
210
96
(96%),
158
pathogens.
Microfluidic-based
had
a
significantly
higher
rate
for
culture
techniques
(96%
vs
38%,
χ2
=
122.0,
P
<
0.01),
it
also
faster
cheaper
than
technology.
ROC
analysis
showed
that
results,
increased
sensitivity
specificity,
similar
In
infectious
diseases,
advantages
time
It
could
critical
drug-resistant
genes
Hence,
can
be
viable
option
diagnosis
treatment
diseases.
The Analyst,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Scheme
of
the
amplification-free
digital
droplet
assay.
Two
innovations:
(1)
polystyrene
capture
beads
to
concentrate
target
RNA
from
a
dilute
sample
and
(2)
“confinement”
effect
amplifies
concentration
molecule.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(11), P. 2417 - 2417
Published: May 21, 2024
Within
the
fields
of
infectious
disease
diagnostics,
microfluidic-based
integrated
technology
systems
have
become
a
vital
in
enhancing
rapidity,
accuracy,
and
portability
pathogen
detection.
These
synergize
microfluidic
techniques
with
advanced
molecular
biology
methods,
including
reverse
transcription
polymerase
chain
reaction
(RT-PCR),
loop-mediated
isothermal
amplification
(LAMP),
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR),
been
successfully
used
to
identify
diverse
array
pathogens,
COVID-19,
Ebola,
Zika,
dengue
fever.
This
review
outlines
advances
detection,
attributing
them
integration
traditional
methods
smartphone-
paper-based
diagnostic
assays.
The
cutting-edge
technologies
are
critical
importance
for
prevention
epidemic
surveillance.
Looking
ahead,
research
is
expected
focus
on
increasing
detection
sensitivity,
streamlining
testing
processes,
reducing
costs,
capability
remote
data
sharing.
improvements
aim
achieve
broader
coverage
quicker
response
mechanisms,
thereby
constructing
more
robust
defense
global
public
health
security.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
6(9)
Published: Aug. 23, 2024
Abstract
The
potential
threat
posed
by
disease
outbreaks
to
diagnostic
instruments
demands
the
development
of
more
effective
biosensor
technologies
counteract
risks.
Diseases
like
SARS-CoV-2,
Ebola,
malaria,
cholera,
and
many
have
demonstrated
beyond
limits
health
care
that
new
advancements
are
required
for
early
detection
diagnosis.
rising
number
diseases
has
led
an
increasing
demand
biosensors
quicker
utilize
in
healthcare
settings.
A
incorporated
with
microfluidic
chips
offers
improved
compared
traditional
or
classical
biosensors.
Microfluidic
improve
performance
allowing
automation,
mixing,
separation,
throughput
processing,
transport
analytes
desired
reactors.
a
chip
sensitivity,
easy
operation
can
use
small
volumes
samples
process
results.
effectiveness
depends
also
on
materials
used
its
fabrication
there
which
reviewed
this
work.
This
paper
reviews
advantages
enhance
biosensors,
fabricate
chips,
electrodes
into
time
shortening
processing
at
point
service.
work
not
previously
addressed
other
including,
integration
cell-imprinted
polymers
sensors
delved
future
outlook.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(9), P. 885 - 885
Published: Sept. 15, 2023
Isothermal
nucleic
acid
amplification
tests
have
recently
gained
popularity
over
polymerase
chain
reaction
(PCR),
as
they
only
require
a
constant
temperature
and
significantly
simplify
amplification.
Recently,
numerous
attempts
been
made
to
incorporate
paper
microfluidics
into
these
isothermal
tests.
Paper
(including
lateral
flow
strips)
used
extract
acids,
amplify
the
target
gene,
detect
amplified
products,
all
toward
automating
process.
We
investigated
literature
from
2020
present,
i.e.,
since
onset
of
COVID-19
pandemic,
during
which
significant
surge
in
has
observed.
microfluidic
detection
extensively
for
recombinase
(RPA)
its
related
methods,
along
with
loop-mediated
(LAMP)
rolling
circle
(RCA).
Detection
was
conducted
primarily
colorimetric
fluorometric
although
few
publications
demonstrated
distance-
surface-enhanced
Raman
spectroscopic
(SERS)-based
detection.
A
good
number
could
be
found
that
both
on
platforms.
small
showed
extraction
or
three
procedures
(i.e.,
fully
integrated
systems)
platforms,
necessitating
need
future
work.
Advances in chemical and materials engineering book series,
Journal Year:
2024,
Volume and Issue:
unknown, P. 206 - 230
Published: Feb. 27, 2024
Nanotechnology
has
emerged
as
a
revolutionary
field
with
diverse
applications,
including
nanocoatings
for
medicinal
purposes.
Nanocoatings,
their
unique
properties
and
capabilities,
have
opened
new
avenues
drug
delivery,
medical
devices,
antimicrobial
treatments.
Nanocoatings
can
be
engineered
to
encapsulate
therapeutic
agents,
protecting
them
from
degradation
facilitating
targeted
delivery
specific
tissues
or
cells.
The
use
of
on
surfaces
also
shown
promising
results
in
preventing
infections
combating
antibiotic-resistant
pathogens.
While
offer
tremendous
potential,
concerns
about
potential
toxicity,
biocompatibility,
long-term
effects
require
rigorous
evaluation
testing.
Furthermore,
the
study
highlights
ongoing
research
future
prospects
nanocoatings.
development
multifunctional
that
combine
sensing
tissue
regeneration
holds
promise
personalized
medicine
advanced
therapeutics.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(6), P. 283 - 283
Published: May 30, 2024
A
microfluidic
immuno-biosensor
detection
system
consisting
of
a
spectrum
chip
and
micro-spectrometer
device
is
presented
for
the
rapid
point-of-care
(POC)
quantification
high-sensitivity
C-reactive
protein
(hs-CRP)
in
urine.
The
process
utilizes
highly
specific
enzyme-linked
immunosorbent
assay
(ELISA)
method,
which
capture
antibodies
are
pre-deposited
on
substrate
microchip
used
to
form
an
immune
complex
with
target
antigen.
Horseradish
peroxidase
(HRP)
added
as
marker
enzyme,
followed
by
colorimetric
reaction
using
3,3',5,5'-tetramethylbenzidine
(TMB).
absorbance
values
(a.u.)
compounds
measured
measure
corresponding
hs-CRP
concentration
according
pre-established
calibration
curve.
It
shown
that
can
be
determined
within
50
min.
In
addition,
achieves
recovery
rates
93.8-106.2%
blind
water
samples
94.5-104.6%
artificial
results
showed
CRP
41
urine
from
patients
chronic
kidney
disease
(CKD)
were
consistent
conventional
homogeneous
particle-enhanced
turbidimetric
immunoassay
(PETIA)
method's
(R
