Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis
Foods,
Journal Year:
2023,
Volume and Issue:
12(18), P. 3355 - 3355
Published: Sept. 7, 2023
Food
analysis
and
control
are
crucial
aspects
in
food
research
production
order
to
ensure
quality
safety
of
products.
Electrochemical
biosensors
based
on
enzymes
as
the
bioreceptors
emerging
promising
tools
for
because
their
high
selectivity
sensitivity,
short
time,
high-cost
effectiveness
comparison
conventional
methods.
This
review
provides
readers
with
an
overview
various
electrochemical
enzyme-based
analysis,
focusing
used
different
applications
sugars,
alcohols,
amino
acids
amines,
organic
acids,
well
mycotoxins
chemical
contaminants.
In
addition,
strategies
improve
performance
that
have
been
reported
over
last
five
years
will
be
discussed.
The
challenges
future
outlooks
sector
also
presented.
Language: Английский
Copper Hexacyanoferrates Obtained via Flavocytochrome b2 Assistance: Characterization and Application
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(3), P. 157 - 157
Published: March 2, 2025
Artificial
enzymes
or
nanozymes
(NZs)
are
gaining
significant
attention
in
biotechnology
due
to
their
stability
and
cost-effectiveness.
NZs
can
offer
several
advantages
over
natural
enzymes,
such
as
enhanced
under
harsh
conditions,
longer
shelf
life,
reduced
production
costs.
The
booming
interest
is
likely
continue
potential
applications
expand.
In
our
previous
studies,
we
reported
the
“green”
synthesis
of
copper
hexacyanoferrate
(gCuHCF)
using
oxidoreductase
flavocytochrome
b2
(Fcb2).
Organic–inorganic
micro-nanoparticles
were
characterized
detail,
including
structure,
composition,
catalytic
activity,
electron-mediator
properties.
An
SEM
analysis
revealed
that
gCuHCF
possesses
a
flower-like
structure
well-suited
for
concentrating
stabilizing
Fcb2.
As
an
effective
peroxidase
(PO)
mimic,
has
been
successfully
employed
H2O2
detection
amperometric
sensors
oxidase-based
biosensors.
current
study,
demonstrated
uniqueness
lies
its
multifunctionality,
serving
PO
chemosensor
ammonium
ions,
biosensor
L-lactate,
exhibiting
perovskite-like
This
exceptional
ability
enhance
fluorescence
blue
light
irradiation
being
first
time.
Using
PO-like
NZ,
novel
developed,
optical
L-arginine
electrochemical
biosensors
methanol
glycerol
determination.
Thus,
gCuHCF,
synthesized
via
Fcb2,
presents
promising
platform
development
biosensors,
bioreactors,
biofuel
cells,
solar
other
advanced
devices.
innovative
approach
utilizing
biocatalysts
nanoparticle
highlights
groundbreaking
direction
materials
science
biotechnology.
Language: Английский
Application of graphene oxide nanosheet lactate biosensors in continuous assessment of athlete fitness
Alexandria Engineering Journal,
Journal Year:
2024,
Volume and Issue:
88, P. 31 - 35
Published: Jan. 11, 2024
This
study
presents
a
groundbreaking
approach
in
sports
science,
introducing
novel
graphene
oxide
(GO)
nanosheet-based
lactate
biosensor
designed
for
the
non-invasive,
continuous
assessment
of
athlete
fitness.
Traditional
monitoring
techniques,
typically
invasive
and
offering
only
intermittent
data,
are
significantly
outperformed
by
this
innovative
method.
Our
uniquely
combines
oxidase
(LOx)
with
GO
nanosheets
to
measure
levels
sweat
unprecedented
accuracy
real-time
response.
Notably,
sensor
exhibits
exceptional
sensitivity
wide
dynamic
range,
effectively
capturing
complete
spectrum
physiological
variations,
from
resting
states
peak
exertion
levels.
The
practical
implications
research
vast,
revolutionary
tool
enhancing
performance
tailoring
training
regimens.
stands
as
testament
potential
nanotechnology
transforming
providing
efficient
solution
precise
threshold
measurement
opening
new
horizons
personalized
athletic
health
monitoring.
Language: Английский
Alleviation of hyperglycaemia and oxidative stress by fruit extracts of different cultivars of the cornelian cherry (Cornus mas L. and Cornus mas × Cornus officinalis) in rats with diabetes mellitus
Food & Function,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Biological
effects
of
fruit
extracts
from
the
different
cultivars
cornelian
cherry
in
rats
with
streptozotocin-induced
diabetes
mellitus.
Language: Английский
Biofuel Cells Based on Oxidoreductases and Electroactive Nanomaterials: Development and Characterization
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(4), P. 249 - 249
Published: April 14, 2025
Amperometric
biosensors
(ABSs)
and
enzymatic
biofuel
cells
(BFCs)
share
several
fundamental
principles
in
their
functionality,
despite
serving
different
primary
purposes.
Both
devices
rely
on
biorecognition,
redox
reactions,
electron
transfer
(ET),
advanced
electrode
materials,
including
innovative
nanomaterials
(NMs).
ABSs
BFCs,
utilizing
microbial
oxidoreductases
combination
with
electroactive
NMs,
are
both
efficient
cost-effective.
In
the
current
study,
laboratory
prototypes
of
BFCs
have
been
developed
bioanodes
based
yeast
flavocytochrome
b2
(Fcb2)
alcohol
oxidase
(AO),
a
cathode
fungal
laccase.
For
first
time,
featuring
anodes
Fcb2
co-immobilized
NMs
glassy
carbon
(GCE),
cathode-utilizing
laccase
combined
gold–cerium–platinum
nanoparticles
(nAuCePt).
The
most
effective
lactate
BFC,
which
contains
gold–hexacyanoferrate
(AuHCF),
exhibited
specific
power
density
1.8
µW/cm2.
A
series
were
an
AO-containing
anode
laccase/nAuCePt/GCE
cathode.
optimal
configuration
featured
bioanode
architecture
AO/nCoPtCu/GCE,
achieving
3.2
constructed
tested
using
lactate-containing
food
product
samples
as
fuels.
Language: Английский
Anaerobic glycolysis and oxidative stress interrelation in erythrocytes under administration of Cornus mas L. fruit extracts to rats with streptozotocin-induced diabetes mellitus
Studia Biologica,
Journal Year:
2024,
Volume and Issue:
18(2), P. 49 - 66
Published: June 1, 2024
Background.
In
diabetes
mellitus
(DM),
analysis
of
changes
in
the
biochemical
profile
erythrocytes
is
important
stage
complex
scientific
research
to
clarify
mechanism
action
medicinal
products
based
on
plant
raw
materials.
The
fruits
Cornus
mas
L.
are
widely
known.
biologically
active
compounds
these
show
multiple
biological
effects.
However,
effect
fruit
extracts
cornelian
cherry
functional
state
has
not
been
sufficiently
studied.
high
glucose
concentration
induces
various
structural
and
changes,
which
lead
numerous
disturbances
their
metabolism.
Glucose
transported
into
by
facilitated
diffusion
via
GLUT2
undergoes
catabolic
breakdown
anaerobic
glycolysis
(90
%
all
glucose)
pentose
phosphate
pathway
(the
rest
10
%).
ATP
reduced
coenzymes
NADH
+
H+
NADPH
formed
due
metabolism
participate
maintaining
structure
hemoglobin.
Enzymes
antioxidant
defense
system,
prevent
hemoglobin
oxidation
methemoglobin,
especially
important.
Hyperglycemia
development
oxidative
stress
cause
a
decrease
activity
enzymes
accumulation
ligand
forms
(HbCO2,
MetHb,
HbA1c).
Therefore,
work
aimed
investigate
red
yellow
content
end
glycolytic
markers
status
blood
cells
rats
with
streptozotocin-induced
diabetes.
Materials
Methods.
DM
1
type
animals
was
induced
intraperitoneal
injection
streptozotocin.
Experiments
were
performed
male
Wistar
rats,
who,
from
10th
day
after
induction,
administered
per
os
loganic
acid
obtained
at
dose
20
mg/kg
body
weight
for
14
days.
On
24th
experiment,
decapitated
under
ether
anesthesia,
taken.
pyruvate
lactate
(as
glycolysis)
L-lactate
dehydrogenase
determined
plasma
erythrocytes,
as
well
(activity
superoxide
dismutase,
catalase
glutathione
peroxidase,
level
glutathione,
TBA-reactive
substances,
modifications
proteins
advanced
protein
products).
Results.
catalase,
peroxidase
significantly
increased
against
proteins,
products,
pyruvate,
L-lactate,
administration
cherry.
Noteworthy,
indicators
made
it
possible
assess
intensity
streptozotocin
Conclusions.
Extracts
might
be
potential
natural
drugs
treatment
metabolic
disorders
diabetes,
they
have
corrective
system
preventing
stress.
It
should
pointed
out
that
extract
showed
best
among
studied
normalizing
indicators.
Language: Английский
Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(11), P. 562 - 562
Published: Nov. 20, 2024
We
report
the
development
of
amperometric
biosensors
(ABSs)
employing
flavocytochrome
Language: Английский