Phytochemical Analysis,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 28, 2024
ABSTRACT
Introduction
The
extraction
of
DNA
is
the
basis
molecular
biology
research.
quality
extracted
one
key
factors
for
success
experiments.
Objective
To
select
a
suitable
method
Chinese
medicinal
herbs
and
seeds.
Methods
In
this
experiment,
four
commercial
kits
were
used
to
extract
genomic
(gDNA)
from
Pinellia
ternata
(Thunb.)
Breit.
powder;
Arisaema
amurense
Maxim.
powder
as
well
seeds
Glycine
max
(L.)
Merr.
On
hand,
concentration
purity
by
these
compared.
other
nucleic
acid
amplification
experiments
performed
on
three
samples
each
Proofman‐LMTIA
methods,
which
novel
isothermal
technique.
different
determine
methods
dry
herbal
medicines
efficiency
curve
show
whether
can
be
in
Results
results
showed
that
high
specificity
optimal
reaction
temperatures
63,
59,
59°C
P.
Makino;
A.
G.
Merr.,
respectively.
gDNAs
with
all
within
acceptable
ranges;
meanwhile,
gDNA
Kit
II
was
highest
efficiency.
Conclusion
principle,
concentration,
purity,
time
taken
extracting
automated
kit
based
magnetic
detection.
SLAS TECHNOLOGY,
Journal Year:
2025,
Volume and Issue:
unknown, P. 100276 - 100276
Published: March 1, 2025
Rapid
and
accurate
detection
of
pathogenic
microorganisms
in
blood
is
critical
for
diagnosing
life-threatening
conditions
such
as
bloodstream
infections
(BSIs).
Current
methods
the
identification
bacteria
from
large
volumes
(5
mL)
involve
culture
steps
followed
by
DNA
extraction/purification/concentration
Polymerase
Chain
Reaction
(PCR)-based
nucleic
acid
amplification.
extraction
amplification
directly
samples
hampered
complexity
matrix,
resulting
time-consuming
labor-intensive
processes.
This
review
delves
into
recent
advancements
molecular
diagnostics
based
on
drying,
coined
'biphasic
reaction',
highlights
this
new
technique
that
attempts
to
overcome
limitations
traditional
sample
preparation
The
biphasic
drying
method,
combination
with
isothermal
loop-mediated
(LAMP)
or
recombinase
polymerase
(RPA),
has
recently
been
shown
improve
sensitivity
bacterial,
viral,
fungal
pathogens
∼1
mL
whole
blood,
while
minimizing
loss
avoiding
use
kits.
Furthermore,
approach
LAMP
be
a
culture-free
method
capable
detecting
clinical
CFU/mL
∼2.5
hours.
represents
significant
reduction
time
compared
current
procedures
bacterial
prior
PCR
paper
aims
guide
identify
opportunities
future
applications
technology.
npj Science of Food,
Journal Year:
2025,
Volume and Issue:
9(1)
Published: March 24, 2025
The
infection
and
outbreak
of
Salmonella
typhimurium
(S.
typhimurium)
highlight
the
need
for
developing
a
reliable
on-site
detection
strategy
fitting
to
various
settings.
However,
due
requirement
specialized
instruments
trained
personnel,
traditional
methods
have
be
implemented
in
laboratories
are
not
ideal
applications.
To
achieve
sample-to-answer
field-deployable
S.
typhimurium,
we
developed
an
integrated
nucleic
acid
platform
combining
single-vial
loop-mediated
isothermal
amplification
(LAMP)-clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/Cas12a
system,
portable
device
smartphone
app.
This
enables
extraction,
concentration,
purification
DNA,
target,
output
visual
fluorescent
signals
within
1
h.
With
this
platform,
102
CFU/mL
food
environmental
matrix
was
able
accurately
detected.
method
demonstrated
excellent
selectivity.
Also,
auxiliary
application
simplified
result
interpretation.
Our
exhibited
potential
better
control
respond
outbreaks
foodborne
diseases,
especially
low-resource
International Journal on Smart Sensing and Intelligent Systems,
Journal Year:
2025,
Volume and Issue:
18(1)
Published: Jan. 1, 2025
Abstract
In
recent
years,
the
point-of-care
testing
(POCT)
device
for
nucleic
acid
detection
has
received
extensive
attention.
POCT
provides
rapid,
high
sensitivity
and
accuracy
of
on-site
diagnosis
can
quantify
in
clinical
samples
real
time.
The
integration
microfluidics
ensures
accurate
fluid
control,
minimizes
sample
volume,
improves
efficiency.
addition,
devices
conduct
multichannel
analysis,
facilitate
continuous
monitoring,
provide
comprehensive
quantitative
data.
Due
to
its
portability,
cost-effectiveness,
ease
use,
represents
a
promising
solution
extensive,
reliable,
timely
various
medical
environments.
This
review
summarizes
research
development
based
on
microfluidic
technology
trend
prospected.
Life,
Journal Year:
2025,
Volume and Issue:
15(5), P. 716 - 716
Published: April 29, 2025
The
search
for
life
beyond
Earth
currently
hinges
on
the
detection
of
biosignatures
that
are
indicative
current
or
past
life,
with
terrestrial
being
sole
known
example.
Deoxyribonucleic
acid
(DNA),
which
acts
as
long-term
storage
genetic
information
in
all
organisms,
is
considered
a
biosignature
life.
Techniques
like
Polymerase
Chain
Reaction
(PCR)
particularly
useful
they
allow
amplification
DNA
fragments,
allowing
even
trace
amounts
material.
This
study
aimed
to
detect
extracted
from
colonies
an
Antarctic
black
fungus
both
when
(i)
alone
and
(ii)
mixed
Sulfatic
Mars
Regolith
Simulant
(S-MRS),
after
exposure
increasing
doses
Fe
ions
(up
1
kGy).
PCR-based
methods
were
used
detection.
findings
this
revealed
no
samples
Simulant,
providing
important
insights
into
potential
application
these
techniques
situ
during
future
space
exploration
missions
their
sample
return
program;
it
also
gives
input
planetary
protection
discussions.
Applied Microbiology,
Journal Year:
2023,
Volume and Issue:
4(1), P. 1 - 15
Published: Dec. 19, 2023
Accurate
pathogenic
detection
in
wastewater
is
critical
for
safeguarding
public
health
and
the
environment.
However,
presence
of
free
nucleic
acids
samples
poses
significant
challenges
to
molecular
accuracy.
This
comprehensive
review
explores
current
status
future
potential
pretreatment
methods
remove
from
samples.
The
study
contributes
a
analysis
mechanisms,
strengths,
limitations
various
approaches,
including
physical,
chemical,
enzymatic
processes.
effect
factors
on
removal
efficiency
these
also
discussed.
enhances
our
comprehension
techniques
their
vital
role
achieving
precise
complex
matrices.
Furthermore,
it
outlines
perspectives
developments
improving
speed
effectiveness
detection,
contributing
significantly
disease
surveillance,
early
warning
systems,
environmental
protection.
Journal of Micromechanics and Microengineering,
Journal Year:
2024,
Volume and Issue:
34(9), P. 095005 - 095005
Published: Aug. 22, 2024
Abstract
Purified
DNA
and
Polymerase
Chain
Reaction
(PCR)
are
crucial
parts
of
molecular
biology
techniques
in
various
fields
such
as
genomics,
forensics,
diagnostics.
The
proposed
microfluidic
device
is
used
to
perform
several
steps
like
the
adsorption
present
processed
PCR
onto
bare
magnetic
beads,
cleaning
contaminants
with
ethanol-diluted
buffer
reagent,
eluting
adsorbed
an
elution
buffer,
which
further
for
downstream
application.
entire
sample
purification
accomplished
about
25
min.
A
comparative
analysis
conducted
using
a
commercially
available
kit.
By
employing
suggested
chip
alongside
commercial
kit,
spectrophotometer
utilized
measure
purity.
This
done
by
obtaining
A260/A280
ratio,
allows
assessment
both
quantity
purity
extracted
DNA.
ratios
spin
column-based,
stand-based,
chip-
based
tests
were
1.86,
1.98,
1.74,
respectively.
eluted
findings
indicated
that
quality
was
suitable
future
amplification.
Additionally,
this
microchip-based
has
potential
be
bedside
point
care
applications,
time
Accurate
pathogenic
detection
in
wastewater
is
critical
for
safeguarding
public
health
and
the
environment.
However,
presence
of
free
nucleic
acids
samples
poses
significant
challenges
to
molecular
accuracy.
This
comprehensive
review
explores
current
status
future
potential
pretreatment
methods
remove
from
samples.
The
study
contributes
a
analysis
mechanisms,
strengths,
limitations
various
approaches,
including
physical,
chemical,
enzymatic
processes.
effect
factors
on
removal
efficiency
these
also
discussed.
enhances
our
comprehension
techniques
their
vital
role
achieving
precise
complex
matrices.
Furthermore,
it
outlines
perspectives
developments
improving
speed
effectiveness
detection,
contributing
significantly
disease
surveillance,
early
warning
systems,
environmental
protection.
