Determination of Stable Reference Genes for Gene Expression Analysis in Black Rockfish (Sebastes schlegeli) Under Hypoxia Stress DOI Open Access

Xiatian Chen,

Yujie Yu, Tao Gao

et al.

Genes, Journal Year: 2024, Volume and Issue: 16(1), P. 9 - 9

Published: Dec. 25, 2024

Hypoxia triggers stress, leading to significant alterations in gene expression patterns, which turn affect fish's growth and development. Real-time quantitative PCR (RT-qPCR) is a pivotal technique for assessing changes expression. However, its accuracy highly contingent upon the stable of reference genes. Ribosomal RNA (18s), β-actin (actb), elongation factor 1-α (ef1a), α tubulin (tuba), ribosomal protein L17 (rpl17) are widely used genes, but their stability tissues black rockfish under hypoxic conditions remains unclear. The genes was detected by RT-qPCR assessed Delta Ct, geNorm, NormFinder, BestKeeper algorithms. Results showed that tuba exhibited liver, heart, gill normoxic conditions, liver head kidney conditions. Ef1a identified as most stably expressed tissue hypoxia. For heart studies, rpl17 were recommended 18s high spleen Actb tissue-specific stability, it necessary select appropriate based on specific type studies These findings help enhancing analysis mechanism hypoxia rockfish.

Language: Английский

Analysis of Personalized Cardiovascular Drug Therapy: From Monitoring Technologies to Data Integration and Future Perspectives DOI Creative Commons

Runxing Lin,

Ziyu Huang, Yu Liu

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 191 - 191

Published: March 17, 2025

Cardiovascular diseases have long been a major challenge to human health, and the treatment differences caused by individual variability remain unresolved. In recent years, personalized cardiovascular drug therapy has attracted widespread attention. This paper reviews strategies for achieving through traditional dynamic monitoring multidimensional data integration analysis. It focuses on key technologies monitoring, based differences, By systematically reviewing relevant literature, main challenges in current research proposed potential directions future studies were summarized.

Language: Английский

Citations

0
Brain organoids-on-chip for neural diseases modeling: History, challenges and trends DOI Creative Commons
Hongyong Zhang, Nan Huang, Sumin Bian

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101323 - 101323

Published: April 1, 2025

Language: Английский

Citations

0
Organotypic on-chip models: Bridging the gap between traditional in vitro culture and animal testing DOI
Letícia Charelli, Tiago Albertini Balbino

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 3 - 10

Published: Jan. 1, 2025

Language: Английский

Citations

0
Recent Advances in Microfluidic Technologies for Stimuli-Sensitive Alginate Particles: Applications in Biosensing and Drug Delivery DOI

Aliakbar Ebrahimiverkiani,

Seyed Morteza Naghib,

Ghobad Shafiei Sabet

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118124 - 118124

Published: Dec. 1, 2024

Language: Английский

Citations

1
Determination of Stable Reference Genes for Gene Expression Analysis in Black Rockfish (Sebastes schlegeli) Under Hypoxia Stress DOI Open Access

Xiatian Chen,

Yujie Yu, Tao Gao

et al.

Genes, Journal Year: 2024, Volume and Issue: 16(1), P. 9 - 9

Published: Dec. 25, 2024

Hypoxia triggers stress, leading to significant alterations in gene expression patterns, which turn affect fish's growth and development. Real-time quantitative PCR (RT-qPCR) is a pivotal technique for assessing changes expression. However, its accuracy highly contingent upon the stable of reference genes. Ribosomal RNA (18s), β-actin (actb), elongation factor 1-α (ef1a), α tubulin (tuba), ribosomal protein L17 (rpl17) are widely used genes, but their stability tissues black rockfish under hypoxic conditions remains unclear. The genes was detected by RT-qPCR assessed Delta Ct, geNorm, NormFinder, BestKeeper algorithms. Results showed that tuba exhibited liver, heart, gill normoxic conditions, liver head kidney conditions. Ef1a identified as most stably expressed tissue hypoxia. For heart studies, rpl17 were recommended 18s high spleen Actb tissue-specific stability, it necessary select appropriate based on specific type studies These findings help enhancing analysis mechanism hypoxia rockfish.

Language: Английский

Citations

0