Portable DNA Probe Detector and a New Dry-QCM Approach for SARS-CoV-2 Detection DOI Creative Commons

Dhanunjaya Munthala,

Thita Sonklin,

Narong Chanlek

et al.

Technologies, Journal Year: 2025, Volume and Issue: 13(3), P. 114 - 114

Published: March 12, 2025

This work demonstrates the preliminary results of rapid and direct detection severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) using quartz crystal microbalance (QCM) method. Coronavirus Disease 2019 (COVID-19)-specific RNA-dependent RNA polymerase (RdRP) gene-dependent probe DNA was used as a selective agent toward target DNA, inactivated SARS-CoV-2 virus, RNAs extracted from clinical samples. study developed utilised unique dry-QCM approach with mitigated experimental procedure. Contact angle measurements, Atomic Force Microscopy (AFM) X-ray Photoelectron Spectroscopy (XPS) measurements were employed to investigate surface during immobilisation hybridisation. also investigates effect temperature on The estimated density 0.51 × 1012 probes/cm2, which is below critical limit. hybridisation efficiency about 58.9%. linear range Limit Detection (LoD) ~1.22 nM high selectivity DNA. sensor shelf-life found be extended 25 days. novelty new for proven results.

Language: Английский

Portable DNA Probe Detector and a New Dry-QCM Approach for SARS-CoV-2 Detection DOI Creative Commons

Dhanunjaya Munthala,

Thita Sonklin,

Narong Chanlek

et al.

Technologies, Journal Year: 2025, Volume and Issue: 13(3), P. 114 - 114

Published: March 12, 2025

This work demonstrates the preliminary results of rapid and direct detection severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) using quartz crystal microbalance (QCM) method. Coronavirus Disease 2019 (COVID-19)-specific RNA-dependent RNA polymerase (RdRP) gene-dependent probe DNA was used as a selective agent toward target DNA, inactivated SARS-CoV-2 virus, RNAs extracted from clinical samples. study developed utilised unique dry-QCM approach with mitigated experimental procedure. Contact angle measurements, Atomic Force Microscopy (AFM) X-ray Photoelectron Spectroscopy (XPS) measurements were employed to investigate surface during immobilisation hybridisation. also investigates effect temperature on The estimated density 0.51 × 1012 probes/cm2, which is below critical limit. hybridisation efficiency about 58.9%. linear range Limit Detection (LoD) ~1.22 nM high selectivity DNA. sensor shelf-life found be extended 25 days. novelty new for proven results.

Language: Английский

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