Enhancing Glioblastoma Resection with NIR Fluorescence Imaging: A Systematic Review DOI Open Access
Hadeel M. Mansour, Siddharth Shah,

Tania M. Aguilar

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(23), P. 3984 - 3984

Published: Nov. 27, 2024

Glioblastoma (GB) is among the most aggressive and difficult-to-treat brain tumors, with a median survival of only 12–15 months despite maximal treatments, including surgery, radiotherapy, chemotherapy. Extensive surgical resection improves in glioblastoma patients; however, achieving complete often hindered by limitations neurosurgical guidance technologies for accurate tumor margin detection. Recent advancements fluorescence-guided surgery (FGS) imaging techniques have significantly enhanced precision extent resections. This study evaluates impact NIR fluorescence on visualization, precision, cost-effectiveness, patient survival. A systematic review PubMed, Scopus, Google Scholar, Embase was conducted to identify studies role surgery. total 135 were included, comprising 10 reviews, three clinical studies, randomized controlled trials (RCTs), preclinical four case reports, all focused The findings indicate that resulting an 18–22% increase gross (GTR) rates studies. provides continuous real-time feedback, minimizing repeat imaging, reducing operational costs, increasing GTR. These improvements contribute better outcomes, extended progression-free survival, improved overall reduced postoperative neurological deficits. underscores potential establish new standard intraoperative management.

Language: Английский

Advancements in NIR-based fluorescence imaging agents for oral oncology: A comprehensive review DOI
Amal Adnan Ashour, Alaa Shafie, Ali Alqarni

et al.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: 235, P. 112637 - 112637

Published: Jan. 8, 2025

Language: Английский

Citations

0
In Vivo Analysis Techniques for Antibody Drug: Recent Advances and Methodological Insights DOI Creative Commons
Xiaodan Miao, Beilei Sun, Jian Zhang

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101314 - 101314

Published: April 1, 2025

Language: Английский

Citations

0
Novel Near Infrared Dyes Targeting Carbonic Anhydrase IX for Fluorescence Imaging Applications DOI

Roberta Napolitano,

Alessia Adamo,

Luca Biondi

et al.

Investigative Radiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Objectives Fluorescence tumor-targeted imaging is a sensitive technique that may assist surgeons to remove residual cancer tissue during resection. Carbonic anhydrase IX (CAIX) tumor-associated cell-surface glycoprotein, upregulated in hypoxic environments, and suitable biomarker develop targeted dyes for fluorescence-guided surgery. This study describes design, synthesis, vitro vivo assessment of novel CAIX-targeting fluorescent probes based on the well-known drug acetazolamide, addressing contribution both targeting moiety fluorophore structure efficacy. Materials Methods All heptamethine cyanines synthesized described present work were characterized terms their optical properties different media. The affinity human serum albumin was evaluated by UV-VIS spectrophotometry. CA catalytic site determined recombinant bovine CAII enzyme (bCAII), with fluorescent-based assay. Human colon adenocarcinoma HT-29 cells, highly expressing CAIX, used characterization, including cell binding, uptake competition assays, flow cytometry. Finally, tumor efficacy selected group assessed Optical Imaging mouse subcutaneous from expression CAIX microenvironment. Results First, family prepared functionalizing glucamine-bearing cyanine (Dye1) modified acetazolamide moiety, whose acetyl replaced i) aminooctanoic acid C8 , ii) phenylalanine, iii) amino-PEG2-acid iv) longer linker 4a or 2 commercially available benzenesulfonamides. From screening this first compounds, C8-AZA due its highest affinity. Indeed, Dye1-C8-AZA exhibited lowest K D values bCAII (6.1 ± 1.6 nM) CAIX-expressing cells (58 9 nM), even lower than HypoxyFluor-1 (HF-1), CAIX-targeted dye already reported literature. Then, other (Dye2–Dye5, linear cyclic, substituents indolenines conjugation position vector) functionalized fully evaluate combinatory effect vector performance resulting probes. chemical features influenced properties, solubility, binding albumin, biodistribution, probes, while leaving unaffected high target. When tested visualization tumors, all showed specific accumulation, often superior HF-1. Conclusions Several test molecular vectors biological properties. containing displayed higher specificity target, strongly also labelling dye. among them especially Dye1-C8-AZA, efficient accumulation. These results support further studies toward clinical testing as target fluorescence pave way future applications.

Language: Английский

Citations

0
Enhancing Glioblastoma Resection with NIR Fluorescence Imaging: A Systematic Review DOI Open Access
Hadeel M. Mansour, Siddharth Shah,

Tania M. Aguilar

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(23), P. 3984 - 3984

Published: Nov. 27, 2024

Glioblastoma (GB) is among the most aggressive and difficult-to-treat brain tumors, with a median survival of only 12–15 months despite maximal treatments, including surgery, radiotherapy, chemotherapy. Extensive surgical resection improves in glioblastoma patients; however, achieving complete often hindered by limitations neurosurgical guidance technologies for accurate tumor margin detection. Recent advancements fluorescence-guided surgery (FGS) imaging techniques have significantly enhanced precision extent resections. This study evaluates impact NIR fluorescence on visualization, precision, cost-effectiveness, patient survival. A systematic review PubMed, Scopus, Google Scholar, Embase was conducted to identify studies role surgery. total 135 were included, comprising 10 reviews, three clinical studies, randomized controlled trials (RCTs), preclinical four case reports, all focused The findings indicate that resulting an 18–22% increase gross (GTR) rates studies. provides continuous real-time feedback, minimizing repeat imaging, reducing operational costs, increasing GTR. These improvements contribute better outcomes, extended progression-free survival, improved overall reduced postoperative neurological deficits. underscores potential establish new standard intraoperative management.

Language: Английский

Citations

2