The effects of chronic, continuous β-funaltrexamine pre-treatment on lipopolysaccharide-induced inflammation and behavioral deficits in C57BL/6J mice DOI Creative Commons

Karissa Hodge,

Daniel J. Buck,

Subhas Das

et al.

Journal of Inflammation, Journal Year: 2024, Volume and Issue: 21(1)

Published: Sept. 2, 2024

Inflammation and neuroinflammation are integral to the progression severity of many diseases strongly associated with cardiovascular disease, cancer, autoimmune disorders, neurodegenerative neuropsychiatric disorders. These can be difficult treat without addressing underlying inflammation, and, as such, a growing need has arisen for pharmaceutical treatments that target inflammatory mediators signaling pathways. Our lab investigated therapeutic potential irreversible µ-opioid antagonist β-funaltrexamine (β-FNA) discovered acute treatment ameliorates inflammation in astrocytes vitro inhibits central peripheral reduces anxiety- sickness-like behavior male C57BL/6J mice. Now, our investigation expanded investigate chronic pre-treatment effects β-FNA on lipopolysaccharide (LPS)-induced Micro-osmotic drug pumps were surgically inserted into subcutaneous intrascapular space or saline vehicle was continuously administered seven days. On sixth day, mice given intraperitoneal injections LPS saline. An elevated plus maze test, followed by forced swim 24 h post-injection measure sickness-, depressive-like behavior. Immediately after testing, frontal cortex, hippocampus, spleen, plasma collected. Levels chemokines C–C motif chemokine ligand 2 (CCL2) C-X-C 10 (CXCL10) measured tissues enzyme-linked immunosorbent assay (ELISA). Quantitative reverse transcription polymerase chain reaction (RT-qPCR) used assess expression enzyme indoleamine 2, 3-dioxygenase 1 (IDO1) NLR family pyrin domain-containing protein 3 (NRLP3) inflammasome cortex spleen tissues. Chronic robustly decreased reduced abolished (e.g., increased time spent motionless, contracted position, distance moved). However, alone both anxiety-like findings provide novel insights anti-inflammatory behavior-modifying continue support under conditions.

Language: Английский

Current Advances in the Therapeutic Potential of Scutellarin: Novel Applications, Mechanisms, and Future Challenges. DOI Creative Commons
Great Iruoghene Edo, Alice Njolke Mafe, Patrick Othuke Akpoghelie

et al.

Phytomedicine Plus, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: Jan. 1, 2025

Language: Английский

Citations

2
Systems Pharmacology Approach and Experiment Evaluation Reveal Pterocarpus Mildbraedii (Fabaceae) Intervention for Counteracting Behavioral Changes and Neuroinflammatory and Oxidative Stress Markers Against Lps-Induced Alzheimer's Disease in Rats DOI
Mengue Ngadena Yolande Sandrine, Pascal Emmanuel Owona,

Armand Fils Ella

et al.

Published: Jan. 1, 2025

Ethnopharmacological relevance: Pterocarpus mildbraedii was believed to have multiple benefits, including antioxidant, antipyretic, antalgic, anti-convulsant, and anxiolytic effects. Previous studies reported that water extract (Pm) contained secondary metabolites able cross the BBB. However, Pm's systemic mechanism targets for neuroinflammation remain largely unexplored.Aim of study: This research used a systems pharmacology approach experiment evaluation reveal potential protective effects Pm against neuroinflammation, oxidative stress, behavioral changes in an LPS-induced Alzheimer's disease (AD) rat model.Materials methods: integrated network analysis experimental verification evaluate pharmacological PM AD systematically. Swiss Target Prediction, GeneCards, STRING databases were employed identify targets. The interaction between active components hub confirmed via molecular docking. GO KEGG pathway analyses also carried out. Further, vitro bioassays explore anti-inflammatory antioxidant activities and, finally, vivo neuroinflammatory stress markers.Results: Network docking revealed primarily regulates signaling pathways such as ESR1, ESR2, BACE1, MAPK1, TLR4, IL6, GSK3B through like liquiritigenin pterocarptriol. identified significant action AD, nitrogen metabolism VEGF pathway. In vitro, demonstrated their properties, along with inhibitory on AchE BchE. Behavioral tests showed LPS exposure impaired exploratory behavior, spatial learning, increased anxiety rats, correlating brain, marked by elevated MDA NO levels, decreased CAT, SOD, GSH levels. raised TNF-α IL-6 levels while reducing dopamine, serotonin, AChE activity. Notably, treatment significantly mitigated improved activity, restored neurotransmitter animals.Conclusion: paper established P. could inhibit its components, targets, pathways. milbraedii may be candidate treatment.

Language: Английский

Citations

0
The effects of chronic, continuous β-funaltrexamine pre-treatment on lipopolysaccharide-induced inflammation and behavioral deficits in C57BL/6J mice DOI Creative Commons

Karissa Hodge,

Daniel J. Buck,

Subhas Das

et al.

Journal of Inflammation, Journal Year: 2024, Volume and Issue: 21(1)

Published: Sept. 2, 2024

Inflammation and neuroinflammation are integral to the progression severity of many diseases strongly associated with cardiovascular disease, cancer, autoimmune disorders, neurodegenerative neuropsychiatric disorders. These can be difficult treat without addressing underlying inflammation, and, as such, a growing need has arisen for pharmaceutical treatments that target inflammatory mediators signaling pathways. Our lab investigated therapeutic potential irreversible µ-opioid antagonist β-funaltrexamine (β-FNA) discovered acute treatment ameliorates inflammation in astrocytes vitro inhibits central peripheral reduces anxiety- sickness-like behavior male C57BL/6J mice. Now, our investigation expanded investigate chronic pre-treatment effects β-FNA on lipopolysaccharide (LPS)-induced Micro-osmotic drug pumps were surgically inserted into subcutaneous intrascapular space or saline vehicle was continuously administered seven days. On sixth day, mice given intraperitoneal injections LPS saline. An elevated plus maze test, followed by forced swim 24 h post-injection measure sickness-, depressive-like behavior. Immediately after testing, frontal cortex, hippocampus, spleen, plasma collected. Levels chemokines C–C motif chemokine ligand 2 (CCL2) C-X-C 10 (CXCL10) measured tissues enzyme-linked immunosorbent assay (ELISA). Quantitative reverse transcription polymerase chain reaction (RT-qPCR) used assess expression enzyme indoleamine 2, 3-dioxygenase 1 (IDO1) NLR family pyrin domain-containing protein 3 (NRLP3) inflammasome cortex spleen tissues. Chronic robustly decreased reduced abolished (e.g., increased time spent motionless, contracted position, distance moved). However, alone both anxiety-like findings provide novel insights anti-inflammatory behavior-modifying continue support under conditions.

Language: Английский

Citations

0