Dynamic Expression of Genes Encoding Ubiquitin Conjugating Enzymes (E2s) During Neuronal Differentiation and Maturation: Implications for Neurodevelopmental Disorders and Neurodegenerative Diseases DOI Open Access

Agathe Paubel,

Sylviane Marouillat, Audrey Dangoumau

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1381 - 1381

Published: Oct. 26, 2024

Background: The ubiquitination process plays a crucial role in neuronal differentiation and function. Numerous studies have focused on the expression functions of E3 ligases during these different stages, far fewer E2 conjugating enzymes. In mice, as humans, E2s belong to 17 enzyme families. Objectives: We analyzed by real-time PCR dynamics all known genes an vitro mouse hippocampal cultures, after, we their stimulation with N-methyl-D-aspartate (NMDA). Results: found that 36 38 were expressed neurons. Many up-regulated neuritogenesis and/or synaptogenesis such Ube2h, Ube2b, Aktip. Rapid delayed responses NMDA associated increased several genes, Ube2i, SUMO-conjugating enzyme. also observed similar profiles within same gene family, consistent presence transcription factor binding sites respective promoter sequences. Conclusions: Our study indicates specific are correlated changes activity. A better understanding regulation function is needed understand played physiological mechanisms pathophysiological alterations involved neurodevelopmental or neurodegenerative diseases.

Language: Английский

Physicochemical Aspects Regarding the Sustainable Conversion of Carwash Slurry as Coverage Admixture for Landfills DOI Open Access
Simona Elena Avram, Lucian Barbu‐Tudoran,

Gheorghe Borodi

et al.

Sustainability, Journal Year: 2025, Volume and Issue: 17(7), P. 2906 - 2906

Published: March 25, 2025

Transport and vehicle traffic are closely connected with particulate matter (PM) pollution, inducing various fractions into the atmosphere, some of them forming significant deposits on surface car. They washed away during carwash-inducing slurries collecting PM deposits, which characteristic a large area. Crystalline was investigated by XRD coupled polarized optical microscopy (POM). Organic matters were Fourier-Transform Infrared spectrometry (FTIR) gas chromatography, GC-MS. Their microstructure elemental composition SEM-EDX. The crystalline features contain mainly quartz, calcite, clay (muscovite kaolinite) particles having traces goethite lepidocrocite. Slurry particle size distribution established sieving following meshes: 63 µm, 125 250 500 1000 2000 4000 µm. Coarse 250–4000 μm dominated quartz calcite particles. amount decreases size, while muscovite kaolinite increases in finest 0–125 μm. evidenced, firstly, FTIR spectroscopy, revealing mostly CH2; C=O, NH4 bonds that more intense for fine fractions. organic form amorphous crusts associated micro- nano-plastic related to phthalates washing detergents. Atomic Force Microscopy revealed their range between 60 90 nm evidenced nanoparticles within samples. nanofractions adhere bigger humid environments, assuring immobilization reduce hazardous potential. Carwash slurry blending fertile soil ensures proper grass seed germination growth at mixtures up 60% slurry, allowing its sustainable reconversion as landfill dump rehabilitation, preventing emission hazard. Blended compositions containing than have noxious effects seeds, inhibiting germination.

Language: Английский

Citations

1
The Neonatal Microbiome: Implications for Amyotrophic Lateral Sclerosis and Other Neurodegenerations DOI Creative Commons
Andrew Eisen, Matthew C. Kiernan

Brain Sciences, Journal Year: 2025, Volume and Issue: 15(2), P. 195 - 195

Published: Feb. 14, 2025

Most brain development occurs in the "first 1000 days", a critical period from conception to child's second birthday. Critical processes that occur during this time include synaptogenesis, myelination, neural pruning, and formation of functioning neuronal circuits. Perturbations first days likely contribute later-life neurodegenerative disease, including sporadic amyotrophic lateral sclerosis (ALS). Neurodevelopment is determined by many events, maturation colonization infant microbiome its metabolites, specifically neurotransmitters, immune modulators, vitamins, short-chain fatty acids. Successful gut-brain axis function depend on maternal factors (stress exposure toxins pregnancy), mode delivery, quality postnatal environment, diet after weaning breast milk, nutritional deficiencies. While neonatal highly plastic, it remains prone dysbiosis which, once established, may persist into adulthood, thereby inducing chronic inflammation abnormal excitatory/inhibitory balance, resulting excitation. Both are recognized as key pathophysiological ALS.

Language: Английский

Citations

0
Mechanism of Nano‐Microplastics Exposure‐Induced Myocardial Fibrosis: DKK3‐Mediated Mitophagy Dysfunction and Pyroptosis DOI
Liang Xiong,

Ziyi Xiong,

Juan Hua

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 22, 2025

ABSTRACT Nano‐microplastics (NMPs), as environmental pollutants, are widely present in nature and pose potential threats to biological health. This study aims investigate the mechanisms by which NMPs inhibit mitophagy through suppression of dickkopf‐related protein 3 (DKK3) expression, leading NOD‐like receptor family, pyrin domain containing (NLRP3) inflammasome‐mediated cardiomyocyte pyroptosis promoting myocardial fibrosis. Healthy adult male C57BL/6 mice were administered NMP solution via gavage, their cardiac function was monitored. The results showed that exposure significantly reduced left ventricular ejection fraction (LVEF) fractional shortening (LVFS) increased extent Transcriptome sequencing identified 14 differentially expressed genes (DEGs), including MYL7. Using random forest algorithm functional enrichment analysis, DKK3 a key gene. In Vitro experiments further confirmed downregulate thereby inhibiting pyroptosis. elucidates molecular induce fibrosis provides new theoretical bases targets for diagnosis treatment heart diseases.

Language: Английский

Citations

0
Microplastics and nanoplastics: fate, transport, and governance from agricultural soil to food webs and humans DOI Creative Commons

Joseph Boctor,

Frances C. Hoyle, Mohamed A. Farag

et al.

Environmental Sciences Europe, Journal Year: 2025, Volume and Issue: 37(1)

Published: May 2, 2025

Language: Английский

Citations

0
From Cradle to Grave: Microplastics—A Dangerous Legacy for Future Generations DOI Open Access

Tamara Lang,

Filip Jelić,

Christian Wechselberger

et al.

Environments, Journal Year: 2024, Volume and Issue: 11(12), P. 263 - 263

Published: Nov. 22, 2024

Microplastics have become a ubiquitous pollutant that permeates every aspect of our environment—from the oceans to soil elementary foundations human life. New findings demonstrate microplastic particles not only pose latent threat adult populations, but also play serious role even before birth during fetal stages development. Exposure microplastics early childhood is another source risk almost impossible prevent. This comprehensive review examines multiple aspects associated with development, detailing mechanisms by which these enter body, their bioaccumulation in tissues throughout life and inevitable re-entry into different ecosystems after death.

Language: Английский

Citations

2
Penetration of Microplastics and Nanoparticles Through Skin: Effects of Size, Shape, and Surface Chemistry DOI Creative Commons
Arianna Menichetti, Dario Mordini, Marco Montalti

et al.

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 15(1), P. 6 - 6

Published: Dec. 31, 2024

Skin represents an effective barrier against the penetration of external agents into human body. Nevertheless, recent research has shown that small particles, especially in nanosized range, can not only penetrate through skin but also work as vectors to transport active molecules such contrast or drugs. This knowledge opened new perspectives on nanomedicine and controlled drug delivery. On other hand, micro- nanoplastics represent a form emerging pollutants, their concentration environment been reported drastically increase last years. The possible these particles become major concern for health. If actual primary toxicity materials is still debated, role toxic skin, originating secondary toxicity, surely alarming. In this review paper, we analyze critically discuss most scientific publications underline how two processes, (i) delivery bioactive by nano-structures (ii) unwanted uncontrolled species mediated nanoparticles, are deeply related efficiency strongly affected nature, size, shape particles.

Language: Английский

Citations

1
Dynamic Expression of Genes Encoding Ubiquitin Conjugating Enzymes (E2s) During Neuronal Differentiation and Maturation: Implications for Neurodevelopmental Disorders and Neurodegenerative Diseases DOI Open Access

Agathe Paubel,

Sylviane Marouillat, Audrey Dangoumau

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1381 - 1381

Published: Oct. 26, 2024

Background: The ubiquitination process plays a crucial role in neuronal differentiation and function. Numerous studies have focused on the expression functions of E3 ligases during these different stages, far fewer E2 conjugating enzymes. In mice, as humans, E2s belong to 17 enzyme families. Objectives: We analyzed by real-time PCR dynamics all known genes an vitro mouse hippocampal cultures, after, we their stimulation with N-methyl-D-aspartate (NMDA). Results: found that 36 38 were expressed neurons. Many up-regulated neuritogenesis and/or synaptogenesis such Ube2h, Ube2b, Aktip. Rapid delayed responses NMDA associated increased several genes, Ube2i, SUMO-conjugating enzyme. also observed similar profiles within same gene family, consistent presence transcription factor binding sites respective promoter sequences. Conclusions: Our study indicates specific are correlated changes activity. A better understanding regulation function is needed understand played physiological mechanisms pathophysiological alterations involved neurodevelopmental or neurodegenerative diseases.

Language: Английский

Citations

0