Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis DOI Creative Commons

Yanyao Deng,

Hongwei Zhu, Le Xiao

et al.

Aging, Journal Year: 2020, Volume and Issue: 13(2), P. 2198 - 2211

Published: Dec. 9, 2020

Circular RNAs (circRNAs) are associated with chemoresistance in many cancers. However, the function of circ_0005198 temozolomide (TMZ) resistance glioma has not been well elucidated. Here, we demonstrated that was considerably up-regulated tissues, serum samples and TMZ-resistant cells. Silencing restrained TMZ resistance, restricted proliferation facilitated apoptosis MiR-198 could be sponged by circ_0005198, effect on progression cells attributed to inhibition miR-198 activity. Moreover, TRIM14 a target silencing hindered suppressed cells, while over-expression rescued inhibiting over-expression. We conclude circ_0005198-miR-198-TRIM14 regulatory pathway is critical glioma.

Language: Английский

The Role of Non-Coding RNA in Congenital Heart Diseases DOI Creative Commons

Ángel Dueñas,

Almudena Expósito,

Amelia Aránega

et al.

Journal of Cardiovascular Development and Disease, Journal Year: 2019, Volume and Issue: 6(2), P. 15 - 15

Published: April 1, 2019

Cardiovascular development is a complex developmental process starting with the formation of an early straight heart tube, followed by rightward looping and configuration atrial ventricular chambers. The subsequent step allows separation these cardiac chambers leading to four-chambered organ. Impairment in any processes invariably leads defects. Importantly, our understanding defects causing congenital diseases has largely increased over last decades. advent molecular era allowed bridge morphogenetic genetic therefore current transcriptional regulation morphogenesis enormously increased. Moreover, impact environmental agents cascades been demonstrated as well novel genomic mechanisms modulating gene such post-transcriptional regulatory mechanisms. Among mechanisms, non-coding RNAs, including therein microRNAs lncRNAs, are emerging play pivotal roles. In this review, we summarize knowledge on functional role RNAs distinct diseases, particular emphasis long RNAs.

Language: Английский

Citations

40
miR-504 modulates the stemness and mesenchymal transition of glioma stem cells and their interaction with microglia via delivery by extracellular vesicles DOI Creative Commons

Ariel Bier,

Hong Xin, Simona Cazacu

et al.

Cell Death and Disease, Journal Year: 2020, Volume and Issue: 11(10)

Published: Oct. 22, 2020

Glioblastoma (GBM) is a highly aggressive tumor with poor prognosis. A small subpopulation of glioma stem cells (GSCs) has been implicated in radiation resistance and recurrence. In this study we analyzed the expression miRNAs associated functions GSCs using miRNA microarray analysis these compared human neural cells. These analyses identified gene clusters cell invasiveness, axonal guidance, TGF-β signaling. miR-504 was significantly downregulated NSCs, its lower GBM normal brain specimens further decreased mesenchymal subtype. Overexpression inhibited their self-renewal, migration markers. The inhibitory effect mediated by targeting Grb10 which acts as an oncogene GBM. exogenous resulted also delivery to cocultured microglia GSC-secreted extracellular vesicles (EVs) abrogation GSC-induced polarization M2 Finally, overexpression prolonged survival mice harboring GSC-derived xenografts growth. summary, potential target networks that play role stemness transition miR-504/Grb10 pathway important regulator process. exerted antitumor effects well bystander on via EVs.

Language: Английский

Citations

38
LncRNA MIR4435‐2HG potentiates the proliferation and invasion of glioblastoma cells via modulating miR‐1224‐5p/TGFBR2 axis DOI Creative Commons

Hongchao Xu,

Beilin Zhang, Yinggui Yang

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2020, Volume and Issue: 24(11), P. 6362 - 6372

Published: April 22, 2020

Abstract Glioblastoma (GBM) belongs to the high‐grade (IV) gliomas with extremely poor prognosis. Accumulating evidence uncovered key roles of long non‐coding RNAs (lncRNAs) in GBM development. This study aimed determine biological actions and clinical relevance lncRNA MIR4435‐2 Host Gene (MIR4435‐2HG) GBM. Data from GEPIA database showed that MIR4435‐2HG was up‐regulated tissues high expression correlated shorter overall survival patients. Further experimental assays verified up‐regulation cell lines. In vitro studies vivo animal knockdown resulted inhibition proliferation invasion tumour growth, while overexpression driven progression. Furthermore, MIR44435‐2HG found sponge miR‐1224‐5p suppress expression; attenuated enhancement induced by overexpression. a subsequent study, target transforming growth factor‐beta receptor type 2 (TGFBR2) repressed TGFBR2 expression, exerted tumour‐suppressive effects via targeting TGFBR2. More importantly, TGFRB2 antagonized hyper‐proliferation cells Clinically, down‐regulation were samples. Taken together, present suggests oncogenic role underlies function MIR4435‐2HG‐driven progression miR‐1224‐5p/TGFBR2 axis.

Language: Английский

Citations

36
LncRNA DGCR5 plays a tumor-suppressive role in glioma via the miR-21/Smad7 and miR-23a/PTEN axes DOI Creative Commons
Zongze He, Juan Long, Chen Yang

et al.

Aging, Journal Year: 2020, Volume and Issue: 12(20), P. 20285 - 20307

Published: Oct. 21, 2020

Glioma is one of the most commonly diagnosed brain malignancies with a high cancer-related death rate in humans. The prognosis glioma patients still unsatisfactory. In present study, we attempted to identify lncRNAs and miRNAs that might be related NF-κB-mediated epithelial-mesenchymal transition cells based on online microarray expression profiles, investigate specific effects lncRNA-miRNA-mRNA axes cell phenotypes. Herein, identified lncRNA DGCR5 as downregulated was negatively regulated by NF-κB1 an RE-dependent manner. LncRNA overexpression significantly inhibited capacity proliferate, migrate, invade, whereas promoted apoptosis cells. Moreover, upregulated epithelial marker E-cadherin while downregulating mesenchymal VIM, well Snai2 TWIST. Regarding underlying molecular mechanisms, could inhibit miR-21 miR-23a expression, or reversed tumor-suppressive overexpression. exerted its through DGCR5/miR-21/Smad7 DGCR5/miR-23a/PTEN axes. conclusion, suppresses migrate invade via miR-21/Smad7, it inhibits proliferation enhances miR-23a/PTEN.

Language: Английский

Citations

35
Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis DOI Creative Commons

Yanyao Deng,

Hongwei Zhu, Le Xiao

et al.

Aging, Journal Year: 2020, Volume and Issue: 13(2), P. 2198 - 2211

Published: Dec. 9, 2020

Circular RNAs (circRNAs) are associated with chemoresistance in many cancers. However, the function of circ_0005198 temozolomide (TMZ) resistance glioma has not been well elucidated. Here, we demonstrated that was considerably up-regulated tissues, serum samples and TMZ-resistant cells. Silencing restrained TMZ resistance, restricted proliferation facilitated apoptosis MiR-198 could be sponged by circ_0005198, effect on progression cells attributed to inhibition miR-198 activity. Moreover, TRIM14 a target silencing hindered suppressed cells, while over-expression rescued inhibiting over-expression. We conclude circ_0005198-miR-198-TRIM14 regulatory pathway is critical glioma.

Language: Английский

Citations

34