Cancers,
Journal Year:
2024,
Volume and Issue:
16(8), P. 1588 - 1588
Published: April 20, 2024
Bladder
cancer
(BC)
diagnosis
is
reliant
on
cystoscopy,
an
invasive
procedure
associated
with
urinary
tract
infections.
This
has
sparked
interest
in
identifying
noninvasive
biomarkers
body
fluids
such
as
blood
and
urine.
A
source
of
these
biofluids
are
extracellular
vesicles
(EVs),
nanosized
that
contain
a
wide
array
molecular
cargo,
including
small
noncoding
RNA
transfer
RNA-derived
fragments
(tRF)
microRNA.
Here,
we
performed
small-RNA
next-generation
sequencing
from
EVs
urine
serum,
well
serum
supernatant.
was
extracted
15
non-cancer
patients
(NCPs)
benign
findings
cystoscopy
41
non-muscle
BC.
Urine
were
collected
before
transurethral
resection
bladder
tumors
(TUR-b)
at
routine
post-surgery
check-ups.
We
compared
levels
tRFs
pre-surgery
samples
to
NCPs
To
further
verify
our
findings,
10
stage
T1
disease
resequenced.
When
comparing
tRF
expression
between
BC
NCPs,
14
differentially
expressed
(DEtRFs)
identified.
In
supernatant,
six
DEtRFs
identified
among
when
four
found
NCPs.
By
performing
blast
search,
sequences
aligned
genomic
pertaining
processes
relevant
development,
enhancers,
regulatory
elements
CpG
islands.
Our
display
number
may
hold
potential
for
the
recurrence-free
survival
Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: Sept. 20, 2022
TRNA-derived
fragments
(tRFs)
and
tRNA-derived
stress-induced
RNAs
(tiRNAs)
are
recognized
as
novel
potential
types
of
non-coding
(ncRNAs),
several
tRF/tiRNA
signatures
closely
associated
with
tumor
diagnosis.
This
study
aimed
to
analyze
the
expression
profiles
plasma
tRFs/tiRNAs
clarify
their
diagnostic
value
in
lung
adenocarcinoma
(LUAD).The
differential
patients
four
early
LUAD,
advanced
healthy
controls
were
analyzed
using
high-throughput
sequencing
technology.
Then,
validated
by
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR),
efficiency
was
appraised
receiver
operating
characteristic
curve
analysis.
The
correlation
candidate
clinicopathological
features
also
analyzed.
Finally,
bioinformatics
analysis
performed
explore
identify
biological
pathways
induced
tRFs/tiRNAs.The
results
revealed
that
from
samples
LUAD
differently
expressed,
supporting
necessity
exploring
biomarkers.
validation
qRT-PCR
demonstrated
level
tRF-1:29-Pro-AGG-1-M6
downregulated
while
tRF-55:76-Tyr-GTA-1-M2
upregulated,
which
consistent
data.
areas
under
0.882
0.896,
respectively,
have
significant
values
diagnosis
LUAD.
expressions
obviously
correlated
various
such
tumor-node-metastasis
stage,
node
levels
carcinoembryonic
antigen.
In
addition,
significantly
altered
before
after
resection
patients.
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes
analyses
further
indicated
widely
distributed
apparently
enriched
tumor-related
signaling
pathways.Plasma
may
be
promising
components
development
highly
sensitive
non-invasive
biomarkers
for
Gene,
Journal Year:
2023,
Volume and Issue:
898, P. 148097 - 148097
Published: Dec. 19, 2023
Transfer
RNAs
(tRNAs)
are
small
non-coding
playing
a
central
role
during
protein
synthesis.
Besides
translation,
growing
evidence
suggests
that
in
many
contexts,
precursor
or
mature
tRNAs
can
also
be
processed
into
smaller
fragments
non-canonical
regulatory
roles
different
biological
pathways
with
oncogenic
relevance.
Depending
on
the
source,
these
molecules
classified
as
tRNA
halves
(also
known
tiRNAs)
tRNA-derived
(tRFs),
and
furtherly
divided
5'-tRNA
3'-tRNA
halves,
tRF-1,
tRF-2,
tRF-3,
tRF-5,
i-tRF,
respectively.
Unlike
DNA
mRNA,
high-throughput
sequencing
of
is
challenging,
because
technical
limitations
currently
developed
methods.
In
recent
years,
approaches
have
been
proposed
allowing
quantification
identification
an
increasing
number
critical
functions
distinct
physiological
pathophysiological
processes.
present
review,
we
discussed
pros
cons
advances
methods,
introducing
expanding
repertoire
bioinformatics
tool
resources
specifically
focused
research
discussing
current
issues
study
RNA
molecules.
Furthermore,
potential
value
diagnostic
prognostic
biomarkers
for
types
cancers.
Cancers,
Journal Year:
2024,
Volume and Issue:
16(8), P. 1588 - 1588
Published: April 20, 2024
Bladder
cancer
(BC)
diagnosis
is
reliant
on
cystoscopy,
an
invasive
procedure
associated
with
urinary
tract
infections.
This
has
sparked
interest
in
identifying
noninvasive
biomarkers
body
fluids
such
as
blood
and
urine.
A
source
of
these
biofluids
are
extracellular
vesicles
(EVs),
nanosized
that
contain
a
wide
array
molecular
cargo,
including
small
noncoding
RNA
transfer
RNA-derived
fragments
(tRF)
microRNA.
Here,
we
performed
small-RNA
next-generation
sequencing
from
EVs
urine
serum,
well
serum
supernatant.
was
extracted
15
non-cancer
patients
(NCPs)
benign
findings
cystoscopy
41
non-muscle
BC.
Urine
were
collected
before
transurethral
resection
bladder
tumors
(TUR-b)
at
routine
post-surgery
check-ups.
We
compared
levels
tRFs
pre-surgery
samples
to
NCPs
To
further
verify
our
findings,
10
stage
T1
disease
resequenced.
When
comparing
tRF
expression
between
BC
NCPs,
14
differentially
expressed
(DEtRFs)
identified.
In
supernatant,
six
DEtRFs
identified
among
when
four
found
NCPs.
By
performing
blast
search,
sequences
aligned
genomic
pertaining
processes
relevant
development,
enhancers,
regulatory
elements
CpG
islands.
Our
display
number
may
hold
potential
for
the
recurrence-free
survival
