Morphological and cytoskeleton changes in cells after EMT DOI Creative Commons
Assel Nurmagambetova,

Vadim V. Mustyatsa,

Aleena A. Saidova

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 13, 2023

Abstract Epithelial cells undergoing EMT experience significant alterations at transcriptional and morphological levels. However, changes in the cytoskeleton, especially cytoskeleton dynamics are poorly described. Addressing question we induced three cell lines (MCF-7, HaCaT A-549) analyzed cytoskeletal there using immunostaining life imaging of transfected with microtubule focal adhesion markers. In all studied lines, area after increased, MCF-7 A-549 became elongated, while kept aspect ratio same. We next components cytoskeleton: microtubules, stress fibers adhesions. The following were observed cultured cells: (i) Organization microtubules becomes more radial; growth rate plus ends was accelerated; (ii) Actin become co-aligned forming longitudinal axis; (iii) Focal adhesions had decreased cancer numerous cells. conclude that among dynamic most during happen regulation microtubules.

Language: Английский

Emerging role of exosomes in cancer progression and tumor microenvironment remodeling DOI Creative Commons
Mahshid Deldar Abad Paskeh, Maliheh Entezari, Sepideh Mirzaei

et al.

Journal of Hematology & Oncology, Journal Year: 2022, Volume and Issue: 15(1)

Published: June 28, 2022

Abstract Cancer is one of the leading causes death worldwide, and factors responsible for its progression need to be elucidated. Exosomes are structures with an average size 100 nm that can transport proteins, lipids, nucleic acids. This review focuses on role exosomes in cancer therapy. We discuss how able modulate components tumor microenvironment influence proliferation migration rates cells. also highlight that, depending their cargo, suppress or promote cell enhance reduce response radio- chemo-therapies. In addition, we describe trigger chronic inflammation lead immune evasion by focusing ability transfer non-coding RNAs between cells other molecular signaling pathways such as PTEN PI3K/Akt cancer. Subsequently, use carriers anti-tumor agents genetic tools control progression. then tumor-derived carcinogenesis. Finally, devote a section study diagnostic prognostic clinical courses important treatment patients. provides comprehensive understanding therapy, therapeutic value remodeling microenvironment. Graphical

Language: Английский

Citations

379
STAT3-EMT axis in tumors: Modulation of cancer metastasis, stemness and therapy response DOI
Mehrdokht Sadrkhanloo, Maliheh Entezari,

Sima Orouei

et al.

Pharmacological Research, Journal Year: 2022, Volume and Issue: 182, P. 106311 - 106311

Published: June 15, 2022

Language: Английский

Citations

115
EMT mechanism in breast cancer metastasis and drug resistance: Revisiting molecular interactions and biological functions DOI Open Access
Mehrdad Hashemi, Hamid Zaferani Arani,

Sima Orouei

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 155, P. 113774 - 113774

Published: Oct. 3, 2022

One of the malignant tumors in women that has involved both developed and developing countries is breast cancer. Similar to other types tumors, cancer cells demonstrate high metastatic nature. Besides, tumor have ability drug resistance. EMT related mechanism metastasis focus current manuscript highlighting function malignancy Breast increase their migration by induction During EMT, N-cadherin vimentin levels increase, E-cadherin decrease mediate EMT-induced invasion. Different kinds anti-cancer agents such as tamoxifen, cisplatin paclitaxel mediates chemoresistance feature cells. Furthermore, correlates with radio-resistance tumor. Clinical aspect reversing preventing chemotherapy or radiotherapy failure patients improving survival time. The anti-tumor suppress can be used for decreasing invasion increasing chemosensitivity lncRNAs, miRNAs factors modulate progression are discussed here.

Language: Английский

Citations

100
Biological impact and therapeutic perspective of targeting PI3K/Akt signaling in hepatocellular carcinoma: Promises and Challenges DOI Creative Commons
Mahshid Deldar Abad Paskeh, Fatemeh Ghadyani, Mehrdad Hashemi

et al.

Pharmacological Research, Journal Year: 2022, Volume and Issue: 187, P. 106553 - 106553

Published: Nov. 16, 2022

Cancer progression results from activation of various signaling networks. Among these, PI3K/Akt contributes to proliferation, invasion, and inhibition apoptosis. Hepatocellular carcinoma (HCC) is a primary liver cancer with high incidence rate, especially in regions prevalence viral hepatitis infection. Autoimmune disorders, diabetes mellitus, obesity, alcohol consumption, inflammation can also lead initiation development HCC. The treatment HCC depends on the identification oncogenic factors that tumor cells develop resistance therapy. present review article focuses role progression. Activation promotes glucose uptake, favors glycolysis increases cell proliferation. It inhibits both apoptosis autophagy while promoting survival. stimulates epithelial-to-mesenchymal transition (EMT) matrix-metalloproteinase (MMP) expression during metastasis. In addition increasing colony formation capacity facilitating spread cells, angiogenesis. Therefore, silencing prevents aggressive behavior. confer drug resistance, particularly sorafenib, decreases radio-sensitivity cells. Anti-cancer agents, like phytochemicals small molecules suppress by limiting Being upregulated tissues clinical samples, be used as biomarker predict patients' response

Language: Английский

Citations

87
Mechanical signatures in cancer metastasis DOI Creative Commons
Ayushi Agrawal,

Yousef Javanmardi,

Sara A. Watson

et al.

npj Biological Physics and Mechanics., Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 4, 2025

Abstract The cancer metastatic cascade includes a series of mechanical barrier-crossing events, involving the physical movement cells from their primary location to distant organ. This review describes changes that influence tumour proliferation, progression, and metastasis. We identify potential signatures at every step discuss some latest mechanobiology-based therapeutic interventions highlight importance interdisciplinary approaches in diagnosis treatment.

Language: Английский

Citations

4
Triple-Negative Breast Cancer Progression and Drug Resistance in the Context of Epithelial–Mesenchymal Transition DOI Open Access
Ewa Błaszczak, Paulina Miziak, Adrian Odrzywolski

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(2), P. 228 - 228

Published: Jan. 12, 2025

Triple-negative breast cancer (TNBC) is one of the most difficult subtypes to treat due its distinct clinical and molecular characteristics. Patients with TNBC face a high recurrence rate, an increased risk metastasis, lower overall survival compared other subtypes. Despite advancements in targeted therapies, traditional chemotherapy (primarily using platinum compounds taxanes) continues be standard treatment for TNBC, often limited long-term efficacy. tumors are heterogeneous, displaying diverse mutation profile considerable chromosomal instability, which complicates therapeutic interventions. The development chemoresistance frequently associated process epithelial–mesenchymal transition (EMT), during epithelial tumor cells acquire mesenchymal-like phenotype. This shift enhances metastatic potential, while simultaneously reducing effectiveness chemotherapeutics. It has also been suggested that EMT plays central role stem cells. Hence, there growing interest exploring small-molecule inhibitors target as future strategy overcoming resistance improving outcomes patients TNBC. review focuses on progression drug emphasis these processes. We present TNBC-specific EMT-related features, key protein markers, various signaling pathways involved. discuss important mechanisms factors related within context EMT, highlighting improve patients’ outcomes.

Language: Английский

Citations

2
Rho-Kinase as a Target for Cancer Therapy and Its Immunotherapeutic Potential DOI Open Access
Seohyun Kim,

Seong A Kim,

Ji-Hoon Han

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(23), P. 12916 - 12916

Published: Nov. 29, 2021

Cancer immunotherapy is fast rising as a prominent new pillar of cancer treatment, harnessing the immune system to fight against numerous types cancer. Rho-kinase (ROCK) pathway involved in diverse cellular activities, and therefore target interest various diseases at level including Indeed, ROCK well-known for its involvement tumor cell microenvironment, especially ability enhance progression, migration, metastasis, extracellular matrix remodeling. Importantly, also considered be novel effective modulator cells, although further studies are needed. In this review article, we describe activities potential utilized particularly immunotherapy, by shining light on system.

Language: Английский

Citations

60
PI3K/AKT Signaling Tips the Balance of Cytoskeletal Forces for Cancer Progression DOI Open Access
Shuo Deng,

Hin Chong Leong,

Arpita Datta

et al.

Cancers, Journal Year: 2022, Volume and Issue: 14(7), P. 1652 - 1652

Published: March 24, 2022

The PI3K/AKT signaling pathway plays essential roles in multiple cellular processes, which include cell growth, survival, metabolism, and motility. In response to internal external stimuli, the co-opts other pathways, components, cytoskeletal proteins reshape individual cells. network comprises three main are namely microfilaments, microtubules, intermediate filaments. Collectively, they for many fundamental structures processes. cancer, aberrant activation of cascade alteration have been observed be highly prevalent, eventually contribute cancer hallmarks. Due their critical tumor progression, pharmacological agents targeting PI3K/AKT, along with developed better intervention strategies against cancer. our review, we first discuss existing evidence in-depth then build on recent advances propose new directions therapeutic intervention.

Language: Английский

Citations

57
Molecular mechanisms behind ROS regulation in cancer: A balancing act between augmented tumorigenesis and cell apoptosis DOI
Hardeep Singh Tuli, Jagjit Kaur,

Kanupriya Vashishth

et al.

Archives of Toxicology, Journal Year: 2022, Volume and Issue: 97(1), P. 103 - 120

Published: Nov. 28, 2022

Language: Английский

Citations

57
Epithelial Mesenchymal Transition (EMT) and Associated Invasive Adhesions in Solid and Haematological Tumours DOI Creative Commons
David Greaves, Yolanda Calle

Cells, Journal Year: 2022, Volume and Issue: 11(4), P. 649 - 649

Published: Feb. 13, 2022

In solid tumours, cancer cells that undergo epithelial mesenchymal transition (EMT) express characteristic gene expression signatures promote invasive migration as well the development of stemness, immunosuppression and drug/radiotherapy resistance, contributing to formation currently untreatable metastatic tumours. The traits associated with EMT can be controlled by signalling nodes at adhesion sites (focal contacts, invadopodia microtentacles) where regulation cell migration, cycle progression pro-survival converge. haematological ample evidence accumulated during last decade indicates an EMT-like phenotype is indicative poor disease prognosis. However, this has not been directly linked assembly specific forms adhesions. current review we discuss role in malignancies examine its possible link towards more aggressive these We also known types adhesions formed speculate on their connection phenotype. postulate understanding architecture EMT-related will lead discovery new therapeutic interventions overcome resistance therapies.

Language: Английский

Citations

46