Detection of antimicrobial resistance via state-of-the-art technologies versus conventional methods DOI Creative Commons
Ayman Elbehiry, Eman Marzouk, Adil Abalkhail

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 25, 2025

Antimicrobial resistance (AMR) is recognized as one of the foremost global health challenges, complicating treatment infectious diseases and contributing to increased morbidity mortality rates. Traditionally, microbiological culture susceptibility testing methods, such disk diffusion minimum inhibitory concentration (MIC) assays, have been employed identify AMR bacteria. However, these conventional techniques are often labor intensive time consuming lack requisite sensitivity for early detection resistance. Recent advancements in molecular genomic technologies-such next-generation sequencing (NGS), matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS), lateral flow immunoassays (LFIAs), PCR-based diagnostic CRISPR-based diagnostics-have revolutionized diagnosis AMR. These innovative approaches provide sensitivity, reduced turnaround times, ability genetic mechanisms. This review seeks examine advantages disadvantages both emerging technologies traditional methods detecting AMR, emphasizing potential benefits limitations inherent each. By understanding strengths technologies, stakeholders, including researchers, healthcare professionals, regulatory agencies, authorities, financial managers, patients, can make informed decisions aimed at preventing emergence dissemination antibiotic-resistant strains, thereby ultimately increasing patient safety.

Language: Английский

Epigallocatechin gallate circumvents drug‐induced resistance in non‐small‐cell lung cancer by modulating glucose metabolism and AMPK/AKT/MAPK axis DOI
Yan Zhou,

Shiqi Huang,

Yizhen Guo

et al.

Phytotherapy Research, Journal Year: 2023, Volume and Issue: 37(12), P. 5837 - 5853

Published: Aug. 24, 2023

Upon prolonged use of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) in non-small-cell lung cancer (NSCLC), acquired drug resistance inevitably occurs. This study investigates the combined EGFR-TKIs (gefitinib or osimertinib) with epigallocatechin gallate (EGCG) to overcome NSCLC models. The vitro antiproliferative effects and EGCG combination EGFR-mutant parental resistant cell lines were evaluated. vivo efficacy was assessed xenograft mouse models derived from EGFR-TKI-resistant cells. We found that significantly reversed Warburg effect by suppressing glycolysis while boosting mitochondrial respiration, which accompanied increased cellular ROS decreased lactate secretion. effectively activated AMPK pathway inhibited both ERK/MAPK AKT/mTOR pathways, leading cycle arrest apoptosis, particularly drug-resistant results obtained tumor model confirmed overcame osimertinib resistance. revealed suppressed bypass survival signaling altered metabolic profiles, is a promising anticancer adjuvant NSCLC.

Language: Английский

Citations

11
Thromboembolic Events Associated with Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors: A Pharmacovigilance Analysis of the US FDA Adverse Event Reporting System (FAERS) Database DOI
Xiongwen Yang, Bo Ram Yang, Dan Li

et al.

Clinical Drug Investigation, Journal Year: 2024, Volume and Issue: 44(3), P. 199 - 207

Published: Feb. 20, 2024

Language: Английский

Citations

4
Mechanisms of resistance to tyrosine kinase inhibitor‐targeted therapy and overcoming strategies DOI Creative Commons
Xuejin Ou, Ge Gao,

Inbar A. Habaz

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(9)

Published: Aug. 24, 2024

Tyrosine kinase inhibitor (TKI)-targeted therapy has revolutionized cancer treatment by selectively blocking specific signaling pathways crucial for tumor growth, offering improved outcomes with fewer side effects compared conventional chemotherapy. However, despite their initial effectiveness, resistance to TKIs remains a significant challenge in clinical practice. Understanding the mechanisms underlying TKI is paramount improving patient and developing more effective strategies. In this review, we explored various contributing resistance, including on-target off-target mechanisms, as well changes histology microenvironment (intrinsic mechanisms). Additionally, summarized current therapeutic approaches aiming at circumventing development of next-generation combination therapies. We also discussed emerging strategies such use dual-targeted antibodies PROteolysis Targeting Chimeras. Furthermore, future directions TKI-targeted therapy, methods detecting monitoring drug during treatment, identification novel targets, exploration dual-acting inhibitors, application nanotechnologies targeted so on. Overall, review provides comprehensive overview challenges opportunities advance our understanding guide treatment.

Language: Английский

Citations

4
The state of the art of EGFR exon 20 insertions in non-small cell lung cancer: Diagnosis and future perspectives DOI Creative Commons

Miriam Dorta-Suárez,

Maria J. de Miguel,

Óscar Amor-Carro

et al.

Cancer Treatment Reviews, Journal Year: 2023, Volume and Issue: 124, P. 102671 - 102671

Published: Dec. 20, 2023

Language: Английский

Citations

10
Detection of antimicrobial resistance via state-of-the-art technologies versus conventional methods DOI Creative Commons
Ayman Elbehiry, Eman Marzouk, Adil Abalkhail

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 25, 2025

Antimicrobial resistance (AMR) is recognized as one of the foremost global health challenges, complicating treatment infectious diseases and contributing to increased morbidity mortality rates. Traditionally, microbiological culture susceptibility testing methods, such disk diffusion minimum inhibitory concentration (MIC) assays, have been employed identify AMR bacteria. However, these conventional techniques are often labor intensive time consuming lack requisite sensitivity for early detection resistance. Recent advancements in molecular genomic technologies-such next-generation sequencing (NGS), matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS), lateral flow immunoassays (LFIAs), PCR-based diagnostic CRISPR-based diagnostics-have revolutionized diagnosis AMR. These innovative approaches provide sensitivity, reduced turnaround times, ability genetic mechanisms. This review seeks examine advantages disadvantages both emerging technologies traditional methods detecting AMR, emphasizing potential benefits limitations inherent each. By understanding strengths technologies, stakeholders, including researchers, healthcare professionals, regulatory agencies, authorities, financial managers, patients, can make informed decisions aimed at preventing emergence dissemination antibiotic-resistant strains, thereby ultimately increasing patient safety.

Language: Английский

Citations

0