Published: Jan. 1, 2024
Language: Английский
Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)
Published: June 20, 2024
Abstract RNA methylation, a prevalent post-transcriptional modification, has garnered considerable attention in research circles. It exerts regulatory control over diverse biological functions by modulating splicing, translation, transport, and stability. Notably, studies have illuminated the substantial impact of methylation on tumor immunity. The primary types encompass N6-methyladenosine (m6A), 5-methylcytosine (m5C), N1-methyladenosine (m1A), N7-methylguanosine (m7G), 3-methylcytidine (m3C). Compelling evidence underscores involvement regulating microenvironment (TME). By affecting translation stability through "writers", "erasers" "readers", influence dysregulation immune cells factors. Consequently, plays pivotal role immunity mediating various behaviors, encompassing proliferation, invasion, metastasis, etc. In this review, we discussed mechanisms several methylations, providing comprehensive overview their roles underlying within among immunocytes. exploring how these modifications mediate evasion, also examine potential applications immunotherapy. This review aims to provide novel insights strategies for identifying targets advancing cancer immunotherapy efficacy.
Language: Английский
Citations
32
Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)
Published: Jan. 17, 2025
Circular RNAs (circRNAs) have garnered substantial attention due to their distinctive circular structure and gene regulatory functions, establishing them as a significant class of functional non-coding in eukaryotes. Studies demonstrated that circRNAs can interact with RNA-binding proteins (RBPs), which play crucial roles tumorigenesis, metastasis, drug response cancer by influencing expression altering the processes tumor initiation progression. This review aims summarize recent advances research on circRNA-protein interactions (CPIs) discuss functions mode action CPIs at various stages expression, including transcription, splicing, translation, post-translational modifications context cancer. Additionally, we explore role resistance gain deeper understanding potential applications development new anti-cancer therapeutic approaches.
Language: Английский
Citations
2
Advanced Science, Journal Year: 2024, Volume and Issue: 11(20)
Published: April 2, 2024
Abstract The modification and recognition of 5‐methylcytosine (m5C) are involved in the initiation progression various tumor types. However, precise role potential mechanism Y‐box‐binding protein 1 (YBX1) esophageal squamous cell carcinoma (ESCC) remains unclear. Here, it is found that YBX1 frequently upregulated ESCC compared with matched nontumor tissues. Gain‐ loss‐of‐function assays show promoted proliferation metastasis cells both vitro vivo. Functional studies revealed NOP2/Sun RNA methyltransferase family member 2 (NSUN2) a critical facilitates YBX1‐mediated progression. Mechanistically, integrated analysis based on immunoprecipitation sequencing (RIP‐seq) m5C methylated (MeRIP‐seq) identified spermine oxidase (SMOX) as target gene containing an site its coding sequence (CDS) region, which coincided well binding YBX1. Overexpression SMOX‐WT but not SMOX‐Mut partially restored invasion ability curbed by knockdown. Moreover, activated mTORC1 signaling pathway stabilizing SMOX mRNA. study reveals promotes development mRNA m5C‐dependent manner, thus providing valuable therapeutic for ESCC.
Language: Английский
Citations
16
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 717 - 717
Published: Jan. 5, 2024
Y-box binding protein 1 (YBX1), a member of the Cold Shock Domain family, is overexpressed in various human cancers and recognized as an oncogenic gene associated with poor prognosis. YBX1’s functional diversity arises from its capacity to interact broad range DNA RNA molecules, implicating involvement diverse cellular processes. Independent investigations have unveiled specific facets contribution cancer development. This comprehensive review elucidates multifaceted role across hallmarks, both cell itself tumor microenvironment. Based on this, we proposed YBX1 potential target for treatment. Notably, ongoing clinical trials addressing breast lung showcased promise therapy. The ramp up vitro research targeting compounds also underscores growing appeal. Moreover, emerging neural input where high level was strongly nerve neurodegenerative diseases. summarized up-to-date advanced pancreatic cancer.
Language: Английский
Citations
13
Environmental Research, Journal Year: 2023, Volume and Issue: 228, P. 115767 - 115767
Published: March 24, 2023
Language: Английский
Citations
17
Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(2)
Published: Feb. 20, 2024
Abstract Breast cancer (BC) is the most commonly diagnosed malignant tumour in females worldwide. Although remarkable advances early detection and treatment strategies have led to decreased mortality, recurrence metastasis remain major causes of death BC patients. Increasing evidence has demonstrated that circular RNAs (circRNAs) play critical roles progression. However, detailed biological functions molecular mechanisms circRNAs are unclear. The aim this study was investigate possible role progression BC. Differentially expressed were identified by integrating breast tumour-associated somatic CNV data circRNA high-throughput sequencing. Aberrant hsa_circ_0007990 expression host gene copy number detected cell lines via quantitative polymerase chain reaction (qPCR). level tissues validated situ hybridization (ISH). Loss- gain-of-function experiments performed vitro vivo, respectively, explore potential function underlying investigated through MS2 RNA pull-down, immunoprecipitation, fluorescence hybridization, immunofluorescence, chromatin immunoprecipitation luciferase reporter assays. levels elevated lines, an effect partly due gains. Functional assays showed promoted growth. Mechanistically, could bind YBX1 inhibit its degradation preventing ubiquitin/proteasome-dependent degradation, thus enhancing cycle-associated E2F1. Rescue suggested YBX1. In general, our modulates ubiquitination protein further regulates E2F1 promote We explored mechanism a novel candidate target for
Language: Английский
Citations
4
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 6, 2025
Esophageal cancer is a major global health challenge, with high incidence and mortality due to the lack of rapid sensitive diagnostic tools specific biomarkers. Cancer-cell-derived extracellular vesicles (EVs) carry unique proteins nucleic acids, making them valuable sources We report an integrated method that combines ultrafast exosome isolation system (EXODUS) matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) detect EVs identify protein biomarkers for diagnosing monitoring esophageal squamous cell carcinoma (ESCC). derived from 20 mL culture medium supernatant ESCC cells varying degrees differentiation serve as analysis models. use EXODUS isolate rapidly. then analyze intact using MALDI-TOF MS, which provides line-specific EV fingerprints in minutes. These allow discrimination normal control enable classification based on degree differentiation. explore critical biomarker peaks diagnosis (5555 m/z, 8603 etc.) (2268 etc.). Potential candidates, including YBX1, DIRAS2, HIST1H2AH, MYBBP1A, are identified through tandem tag (TMT) proteomics. tentatively assign identities marker by correlation TMT Applying this plasma-derived shows promise rapid, minimally invasive ESCC.
Language: Английский
Citations
0
Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)
Published: Dec. 18, 2023
Abstract RNA methylation modification plays a crucial role as an epigenetic regulator in the oncogenesis of hepatocellular carcinoma (HCC). Numerous studies have investigated molecular mechanisms underlying protein-coding RNAs progression HCC. Beyond their impact on mRNA, modifications also influence biological functions non-coding (ncRNAs). Here, we present advanced and comprehensive overview interplay between ncRNAs HCC, with specific focus potential implications for tumor immune microenvironment. Moreover, summarize promising therapeutic targets HCC based methylation-related proteins. In future, more profound investigation is warranted to elucidate effects ncRNA pathogenesis devise valuable intervention strategies.
Language: Английский
Citations
8
iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 107818 - 107818
Published: Sept. 4, 2023
Many circular RNAs (circRNAs) have been identified to be associated with hepatocellular carcinoma (HCC) progression. We aim explore the diagnostic potential, functions, and mechanism of circELMOD3 in HCC. Differentially expressed circRNAs HCC its paired adjacent tissues were by RNA sequencing. was downregulated related clinicopathological characteristics patients. Additionally, plasma shown a highly sensitive non-invasive biomarker distinguish from healthy controls. Functional assays showed that inhibited proliferation induced apoptosis cells both vitro vivo. Mechanistically, antisense purification (RAP) luciferase reporter verified functioned as sponge for miR-6864-5p leading increased expression target gene TRIM13. Interestingly, stability test demonstrated overexpression led enhanced directly bound TRIM13 mRNA, which turn co-activated p53 signaling pathway.
Language: Английский
Citations
4
Gene, Journal Year: 2024, Volume and Issue: unknown, P. 148971 - 148971
Published: Sept. 1, 2024
Language: Английский
Citations
1