Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 4, 2024
Abstract
5-
methylcytosine
(m5C)
is
a
prevalent
posttranscriptional
RNA
modification
which
participates
in
the
initiation
and
progression
of
various
cancers.
NSUN2
main
methyltransferase
import
for
catalyzing
m5C
formation.
However,
its
regulatory
role
potential
mechanism
osteosarcoma
remain
unclear.
Here,
we
demonstrated
that
expression
was
markedly
upregulated
tissues
cell
lines.
Clinically,
increased
associated
with
poor
prognosis.
Functional
studies
revealed
significantly
promoted
metastasis
epithelial-mesenchymal
transition
(EMT)
osteosarcoma.
Mechanistically,
integrated
analysis
based
on
sequencing
correlation
identified
UBE2S
as
target
downstream
gene
NSUN2,
while
enhanced
mRNA
stabilization
an
m5C-dependent
manner.
More
importantly,
UBE2S
overexpression
reversed
inhibition
invasion
EMT
induced
by
knockdown.
Moreover,
interacted
ubiquitinated
β-catenin,
enhancing
stability
activation.
Interestingly,
patients
dual-high
exhibited
shorter
overall
survival.
In
summary,
our
study
facilitated
UBE2S/β-catenin
axis,
suggesting
therapeutic
approach
