Highly Sensitive Chemiluminescent Probe for CYP 2J2 Detection and Image-Guided Liver Cancer Surgery DOI
Cuicui Jiang,

Tingting Geng,

Pengfei Fang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19560 - 19566

Published: Nov. 25, 2024

Developing chemiluminescent (CL) probes with a high tumor-to-normal tissue ratio is crucial for liver tumor visualization and image-guided surgery. Cytochrome P450 2J2 (CYP 2J2), an extrahepatic enzyme, highly expressed in tumors but not healthy tissues. However, no prior reports have documented the development of CL specifically targeting CYP 2J2. In this study, we designed 2J2-responsive probe, termed CYP-PD, by grafting methoxybenzyl alcohol onto Schaap's dioxetanes. vitro cellular experiments confirmed that CYP-PD selectively sensitively responds to both exogenous endogenous 2J2, exhibiting extraordinary limit detection 1.21 pM threshold as low 235 cells. Moreover, demonstrated highest intensity HepG-2 cells, ∼59.6-fold enhancement relative normal cells (L02). Notably, probe enabled lesions from tissues (∼44.7-fold), successfully guiding surgical resection orthotopic cancer mouse model. We envision work may provide powerful tool surgery future.

Language: Английский

The Alcatraz‐Strategy: a roadmap to break the connectivity barrier in malignant brain tumours DOI Creative Commons
Matthias Schneider, Anna‐Laura Potthoff, Georg Karpel‐Massler

et al.

Molecular Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: April 3, 2024

In recent years, the discovery of functional and communicative cellular tumour networks has led to a new understanding malignant primary brain tumours. this review, authors shed light on diverse nature cell‐to‐cell connections in tumours propose an innovative treatment approach address detrimental connectivity these networks. The proposed therapeutic outlook revolves around three main strategies: (a) supramarginal resection removing substantial portion communicating cell front far beyond gadolinium‐enhancing mass, (b) morphological isolation at single level disrupting structural contacts facilitated by elongated membrane protrusions known as microtubes (TMs), (c) blocking TM‐mediated intercellular cytosolic exchange inhibiting neuronal excitatory input into network. We draw analogy between Alcatraz Federal Penitentiary, where inmates faced impassable sea barrier experienced both spatial within individual cells. Based current translational efforts ongoing clinical trials, we Alcatraz‐Strategy promising framework tackle harmful effects

Language: Английский

Citations

7
Awake Craniotomy for Gliomas in the Non-Dominant Right Hemisphere: A Comprehensive Review DOI Open Access
Dilshod Mamadaliev, Ryuta Saito, Kazuya Motomura

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(6), P. 1161 - 1161

Published: March 15, 2024

Awake surgery has become a standard practice for managing diffuse low-grade gliomas (LGGs), particularly in eloquent brain areas, and is established as gold technique left-dominant-hemisphere tumors. However, the intraoperative monitoring of functions right non-dominant hemisphere (RndH) often neglected, highlighting need better understanding neurocognitive testing complex hemisphere. This article aims to comprehensively review current literature on benefits awake craniotomy A systematic was conducted using PubMed ScienceDirect databases with keywords such "right hemisphere", "awake surgery", "direct electrical stimulation mapping", "glioma". The search focused anatomical surgical aspects, including indications, tools, techniques cerebral gliomas. identified 74 sources, original articles, books, monographs, articles. Two papers reported large series language assessment cases 246 patients undergoing detailed neurological semiology mapping techniques, while remaining studies were predominantly neuroradiological neuroimaging nature. non-dominant-hemisphere an essential tool. term "non-dominant" should be revised, this contributes significantly cognitive human brain.

Language: Английский

Citations

5
From non-human to human primates: a translational approach to enhancing resection, safety, and indications in glioma surgery while preserving sensorimotor abilities DOI Creative Commons

Matteo Gambaretti,

Luca Viganò, Matteo Gallo

et al.

Frontiers in Integrative Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: Feb. 10, 2025

Since the pivotal studies of neurophysiologists in early 20th century, research on brain functions non-human primates has provided valuable insights into neural mechanisms subserving neurological function. By using data acquired as a reference, important progress knowledge human and its been achieved. The translational impact allowed by this scientific effort must be recognized implementation current surgical techniques particularly support neurosurgical approach to tumors. In treatment tumors, ability maximally extend resection allows an improvement overall survival, progression-free quality life patients. main goal, and, at same time, challenge, oncological surgery is avoid permanent deficit while reaching maximal resection, when tumor infiltrates network motor functions. Brain mapping were developed neurophysiological probes identify areas tracts sensorimotor function, ensuring their preservation during resection. During last 20 years, starting from classical “Penfield” technique, progressively implemented. Among major advancements was introduction high-frequency direct electrical stimulation. Its refinement, along with complementary use low-frequency stimulation, further refinement stimulation protocols. narrative review, we propose analysis process through which experiments influenced changed procedures. We then describe used tumors located within circuits. also detail how these acquisition new properties underlying control, turn fostering design tools navigate cortical subcortical areas, that before deemed “sacred untouchable.”

Language: Английский

Citations

0
The urgent need to redefine quality of life in patients with low-grade glioma DOI Creative Commons
Hugues Duffau

ESMO rare cancers., Journal Year: 2025, Volume and Issue: unknown, P. 100003 - 100003

Published: Feb. 1, 2025

Language: Английский

Citations

0
Postoperative Neurologic Outcome in Patients Undergoing Resective Surgery for Parietal Lobe Epilepsy DOI
Stuart Gates, Dawn E. Hackman, Nitin Agarwal

et al.

Neurology, Journal Year: 2024, Volume and Issue: 102(12)

Published: May 30, 2024

Parietal lobe epilepsy (PLE) surgery can be an effective treatment for selected patients with intractable but associated the risk of serious neurologic deficits. We performed a systematic review literature to obtain comprehensive summary frequency and types new postoperative deficits in undergoing PLE resective surgery.

Language: Английский

Citations

3
Awake Craniotomy for Diffuse Low Grade Gliomas in a Resource Limited Setting: Lessons Learned with a Consecutive Series of 51 Surgeries DOI
Lucas Alverne Freitas de Albuquerque, Leonardo de Macêdo Filho, Felipe S. Borges

et al.

World Neurosurgery, Journal Year: 2023, Volume and Issue: 177, P. e563 - e579

Published: June 28, 2023

Language: Английский

Citations

3
Vers un modèle en méta-réseau du fonctionnement cérébral : contribution de la chirurgie éveillée des gliomes de bas grade et implications thérapeutiques DOI
Hugues Duffau

Bulletin de l Académie Nationale de Médecine, Journal Year: 2024, Volume and Issue: 208(6), P. 824 - 831

Published: April 26, 2024

Citations

0
DTI Analysis of the Peritumoral Zone of Diffuse Low-grade Gliomas in Progressing Patients DOI Creative Commons
Dylan Chiche, Luc Taillandier,

Marie Blonski

et al.

World Neurosurgery, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Diffuse low-grade gliomas are rare brain tumors transforming to higher grade even with surgery, chemotherapy, and radiotherapy. Their preferential infiltration of white matter tracts, beyond tumor boundaries on fluid-attenuated inversion recovery (FLAIR), make difficult plan focal treatment such as surgery or radiotherapy monitor response chemotherapy. Diffusion tensor imaging (DTI) might reflect this tracts. The aim our study is assess how DTI signal in the peritumoral zone be modified before FLAIR progression appears at 1-year follow-up.

Language: Английский

Citations

0
Highly Sensitive Chemiluminescent Probe for CYP 2J2 Detection and Image-Guided Liver Cancer Surgery DOI
Cuicui Jiang,

Tingting Geng,

Pengfei Fang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19560 - 19566

Published: Nov. 25, 2024

Developing chemiluminescent (CL) probes with a high tumor-to-normal tissue ratio is crucial for liver tumor visualization and image-guided surgery. Cytochrome P450 2J2 (CYP 2J2), an extrahepatic enzyme, highly expressed in tumors but not healthy tissues. However, no prior reports have documented the development of CL specifically targeting CYP 2J2. In this study, we designed 2J2-responsive probe, termed CYP-PD, by grafting methoxybenzyl alcohol onto Schaap's dioxetanes. vitro cellular experiments confirmed that CYP-PD selectively sensitively responds to both exogenous endogenous 2J2, exhibiting extraordinary limit detection 1.21 pM threshold as low 235 cells. Moreover, demonstrated highest intensity HepG-2 cells, ∼59.6-fold enhancement relative normal cells (L02). Notably, probe enabled lesions from tissues (∼44.7-fold), successfully guiding surgical resection orthotopic cancer mouse model. We envision work may provide powerful tool surgery future.

Language: Английский

Citations

0