Therapeutic Effect of Cisplatin-Loaded Polymeric Nanoparticles and Nimotuzumab Combination in Human Glioblastoma Cells

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Objective This study evaluated the combined therapeutic effects of cisplatin-loaded PEG-PLGA nanoparticles (CIS-PEG-PLGA) and nimotuzumab (NIMO) on glioblastoma (GBM) cells, focusing their antiproliferative, apoptotic, cell cycle-modulatory effects. Materials Methods were synthesized using a double-emulsion solvent evaporation method, achieving mean particle size ~ 450 nm. Cisplatin release kinetics, proliferation, apoptosis, cycle progression assessed in U87 cells. The treatments included free cisplatin (CIS), CIS-PEG-PLGA, NIMO, combinations. Experiments performed triplicate, statistical analyses ANOVA test. Results CIS-PEG-PLGA exhibited sustained release, with 50% released within 8 h nearly 100% 72 h. combination + NIMO reduced proliferation by 90%, significantly surpassing that CIS (50%) (70%). Apoptosis rates highest group (27.52%), followed (18.59%) (25.95%). Cell analysis revealed significant G2 phase arrest (30.12%) treatment group, highlighting its superior modulatory progression. Conclusion demonstrated synergistic enhancing outcomes. approach represents promising advancement therapy integrating nanotechnology immunotherapy to overcome conventional limitations. Further, vivo studies are warranted validate these findings explore clinical applications.

Language: Английский

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GENOMIC AND TRANSCRIPTOMIC ALTERATIONS IN THE ELN GENE NETWORK AND THEIR ASSOCIATION WITH HEAD AND NECK SQUAMOUS CELL CARCINOMA

BULLETIN OF STOMATOLOGY AND MAXILLOFACIAL SURGERY, Journal Year: 2025, Volume and Issue: unknown, P. 230 - 238

Published: March 7, 2025

Head and neck squamous cell carcinoma (HNSCC) are a common form of cancer that arises in the mucosal surfaces various anatomical locations, including larynx, oral cavity, nasal cavity. The ELN gene plays crucial role elastin synthesis, protein responsible for maintaining elasticity skin, blood vessels, ligaments, thereby preventing senescence. Genetic alterations family may contribute to development progression HNSCC. Objectives: This study aims investigate their potential association with Materials Methods: Computational analyses were performed using publicly available databases, UALCAN, cBioPortal, STRING, identify genetic changes HNSCC patients. focused on mutations (missense truncating), amplification, deep deletions within family. Results: analysis revealed high frequency member 11, occurring 5% Additionally, significant variation expression was observed between normal samples different grades. Kaplan-Meier survival indicated an insignificant difference MFAP2 but FN1 primary tumour groups Conclusion: These findings suggest link HNSCC, emphasizing necessity further experimental validation confirm these preliminary observations elucidate underlying mechanisms.

Language: Английский

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Epithelial‑derived head and neck squamous tumourigenesis (Review)

Oncology Reports, Journal Year: 2024, Volume and Issue: 52(4)

Published: Aug. 21, 2024

Language: Английский

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Peroxisome proliferator-activated receptorα/γ agonist pioglitazone for rescuing relapsed or refractory neoplasias by unlocking phenotypic plasticity

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 11, 2024

A series of seven clinical trials on relapsed or refractory (r/r) metastatic neoplasias followed the question: Are networks ligand-receptor cross-talks that support tumor-specific cancer hallmarks, druggable with tumor tissue editing approaches therapeutically exploiting plasticity? Differential recombinations pioglitazone, a dual peroxisome-proliferator activated receptor α/γ (PPARα/γ) agonist, transcriptional modulators, i.e., all-trans retinoic acid, interferon-α, dexamethasone plus metronomic low-dose chemotherapy (MCT) epigenetic modeling azacitidine plus/minus cyclooxygenase-2 inhibition initiated reprogramming as exemplified by inflammation control in r/r melanoma, renal clear cell carcinoma (RCCC), Hodgkin’s lymphoma (HL) and multisystem Langerhans histiocytosis (mLCH) differentiation induction non-promyelocytic acute myeloid leukemia (non-PML AML). Pioglitazone, integrated differentially designed schedules, facilitated death indicated complete remission (CR) non-PML AML, continuous CR RCCC, mLCH, HL addition everolimus, long-term disease melanoma efficaciously controlling metastasis, post-therapy repopulation acquired cell-resistance genetic/molecular-genetic heterogeneity (M-CRAC). PPARα/γ agonists provided tumor-type agnostic biomodulatory efficacy across different histologic neoplasias. Tissue techniques disclose wide-ranging functions may be on-topic focused for unlocking phenotypes. Low-dose MCT facilitates targeted hallmarks death, M-CRAC non-oncogene addiction. Thus, protocols, is an important drug addressing urgent therapeutic problems, such disease.

Language: Английский

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Evaluation of Five-year Overall Survival Rates Among 18,331 Head and Neck Cancer Patients Exposed to Different Targeted Therapies Through Real-world Data in a Case-controlled Study

Anticancer Research, Journal Year: 2024, Volume and Issue: 44(3), P. 1247 - 1270

Published: Feb. 29, 2024

Background/Aim: Targeted therapy is an important and fast developing aspect of modern tumor including head neck cancer (HNC). Surgically treated patients often experience significant limitations to their ability swallow, speak, or mimic expressions. In cases recurrent tumors palliative situations, targeted therapies such as immune checkpoint inhibitors (ICI) are frequently employed. This study compared different focusing on survival probability. Patients Methods: Data from with regimens the TriNetX network were analyzed. Two groups formed: Cohort I received one therapy, whereas in cohort II a therapy. Cohorts matched 1:1 respect certain confounders. After defining primary outcome "death", Kaplan–Meier analysis was performed, risk ratio (RR), odds (OR), hazard (HR) calculated. Results: A total 18,331 HNC VEGF had significantly longer overall than c-MET EGFR inhibitors. PI3K showed reduced probability those c-MET, mTOR, RET Conclusion: most used HNC. However, present analysis, advantage observed

Language: Английский

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PRI-724 and IWP-O1 Wnt Signaling Pathway Inhibitors Modulate the Expression of Glycolytic Enzymes in Tongue Cancer Cell Lines

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(12), P. 9579 - 9592

Published: Nov. 29, 2023

The dysregulation of energetic metabolism is one the hallmarks cancer cells. Indeed, growth head and neck squamous cell carcinoma (HNSCC) cells depends heavily on glycolytic activity, which can be considered a potential therapeutic target. Wnt signaling pathways that undergoes upregulation in HNSCC. Our previous studies have shown inhibitors-PRI-724 IWP-O1-attenuate tongue SCC survival reduce glucose uptake lactate release. aim this research was to further evaluate possible mechanisms previously observed effects. We assessed effect PRI-724 IWP-O1 expression selected enzymes: phosphofructokinase M, pyruvate kinase M2, dehydrogenase. Relative transcript by real-time PCR, protein levels Western blot. Moreover, clinical data concerning mRNA expression, gene promoter methylation, HNSCC patients' time were analyzed UALCAN tool, protein-protein interaction using STRING database. Experimental bioinformatic confirmed relation between enzymes samples. Overall, inhibition inhibitors promising mode action against

Language: Английский

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Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Reports of Practical Oncology & Radiotherapy, Journal Year: 2024, Volume and Issue: unknown

Published: March 9, 2024

Language: Английский

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