Cell Division,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: Nov. 28, 2024
Cervical
carcinoma
poses
a
significant
health
threat,
with
traditional
treatments
proving
inadequate
in
advanced
stages.
Curcumin,
bioactive
compound
derived
from
turmeric,
exhibits
notable
anti-inflammatory,
antioxidant,
and
antineoplastic
properties,
potentially
modulating
autophagy,
metastasis
cancer
cells.
This
study
examines
curcumin's
impact
on
autophagy
cervical
carcinoma,
focusing
its
interaction
autophagy-related
gene
3
(ATG3).
SiHa
HeLa
cell
lines
were
treated
curcumin,
ATG3
knockdown
(shATG3),
their
combination.
Cell
migration
was
evaluated
via
wound
healing
assays,
while
proliferation
CCK-8
assays.
LC3
expression
assessed
using
immunofluorescence
western
blotting.
Molecular
docking
simulations
identified
binding
interactions
key
proteins.
Curcumin
shATG3
significantly
inhibited
both
proliferation,
synergistic
effect
observed
when
combined.
enhanced,
indicating
increased
autophagy.
Docking
studies
revealed
potential
to
MMP2,
MMP9,
TGF-β,
ATG3,
LC3,
p62,
suggesting
modulation
of
these
pathways.
The
combination
curcumin
also
enhancing
supporting
the
as
therapeutic
agent
for
carcinoma.
Further
clinical
research
is
needed
validate
findings.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(20), P. 11201 - 11201
Published: Oct. 18, 2024
CD133
protein
expression
is
observable
in
differentiated
cells,
stem
and
progenitor
cells
within
normal
tissues,
as
well
tumor
including
colorectal
cancer
cells.
The
the
predominant
cell
surface
marker
utilized
to
detect
exhibiting
cell-like
characteristics.
alters
common
abnormal
processes
cancer,
such
phosphoinositide
3-kinase
(PI3K)/protein
kinase
B
(AKT)
Wnt/β-catenin
pathways.
Autophagy
a
cellular
self-digestion
mechanism
that
preserves
intracellular
milieu
plays
dual
regulatory
role
cancer.
In
apoptosis
critical
death
can
impede
progression.
modulate
autophagy
via
several
signaling
pathways;
hence,
it
involved
regulation
of
these
intricate
processes.
This
be
an
explanation
for
why
associated
with
enhanced
self-renewal,
migration,
invasion,
survival
under
stress
conditions
purpose
this
review
article
explain
complex
relationship
between
protein,
apoptosis,
autophagy.
We
also
want
highlight
possible
ways
CD133-mediated
may
affect
Targeting
aforementioned
mechanisms
have
significant
therapeutic
eliminating
CD133-positive
cell-phenotype
which
responsible
recurrence.
Cell Division,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: Nov. 28, 2024
Cervical
carcinoma
poses
a
significant
health
threat,
with
traditional
treatments
proving
inadequate
in
advanced
stages.
Curcumin,
bioactive
compound
derived
from
turmeric,
exhibits
notable
anti-inflammatory,
antioxidant,
and
antineoplastic
properties,
potentially
modulating
autophagy,
metastasis
cancer
cells.
This
study
examines
curcumin's
impact
on
autophagy
cervical
carcinoma,
focusing
its
interaction
autophagy-related
gene
3
(ATG3).
SiHa
HeLa
cell
lines
were
treated
curcumin,
ATG3
knockdown
(shATG3),
their
combination.
Cell
migration
was
evaluated
via
wound
healing
assays,
while
proliferation
CCK-8
assays.
LC3
expression
assessed
using
immunofluorescence
western
blotting.
Molecular
docking
simulations
identified
binding
interactions
key
proteins.
Curcumin
shATG3
significantly
inhibited
both
proliferation,
synergistic
effect
observed
when
combined.
enhanced,
indicating
increased
autophagy.
Docking
studies
revealed
potential
to
MMP2,
MMP9,
TGF-β,
ATG3,
LC3,
p62,
suggesting
modulation
of
these
pathways.
The
combination
curcumin
also
enhancing
supporting
the
as
therapeutic
agent
for
carcinoma.
Further
clinical
research
is
needed
validate
findings.
