Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 3, 2024
Language: Английский
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16
Published: April 1, 2025
Cuproptosis, along with RNA methylation regulators, has recently come to the fore as innovative mechanisms governing cell death, exerting profound impact on onset and progression of multiple cancers. Nonetheless, prognostic implications underlying regulatory them associated prostate cancer (PCa) remain be thoroughly investigated. Genomic clinical data for PCa from The Cancer Genome Atlas datasets were analyzed identify a model through univariate Least Absolute Shrinkage Selection Operator Cox regression analyses that validated utilizing external datasets. We used receiver operating characteristic curves C-index evaluate accuracy our model. In conjunction this, we conducted single-cell sequencing (scRNA-seq) investigate degree immune infiltration, well assess patients' responses diverse chemotherapy agents. Especially, qPCR assay was utilized unveil expression signature genes in PCa. meticulously selected six Cuproptosis-Associated Methylation Regulators (CARMRs) establish risk prognosis model, which further verified obtain enhanced predictive capacity validation cohorts. Insights infiltration scRNA-seq have elucidated characteristics PCa, highlighted immunosuppressive role T cells response. Additionally, drug susceptibility analysis demonstrated patients low-risk category derived better benefit bicalutamide treatment, whereas those high-risk group exhibited favor response adriamycin docetaxel treatments. immunohistochemistry (IHC) staining assays also reveal dramatically altered pattern TRDMT1 ALYREF tissues. general, established involving CARMRs can predict recurrence identified possible affecting progression, thereby promoting research this field.
Language: Английский
Citations
0
Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 189, P. 110000 - 110000
Published: March 8, 2025
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: July 23, 2024
Abstract Background: Prostate cancer, a common malignancy, is driven by androgen receptor (AR) signaling. Understanding the function of AR signaling critical for prostate cancer research. Methods: We performed multi-omics data analysis + , androgen-sensitive LNCaP cell line, focusing on gene expression (RNAseq), chromatin accessibility (ATACseq), and transcription factor binding (ChIPseq). High-quality datasets were curated from public repositories processed using state-of-the-art bioinformatics tools. Results: Our identified 1004 up-regulated 707 down-regulated genes in response to deprivation therapy (ADT) which diminished activity. Gene-set enrichment revealed that influences pathways related neuron differentiation, adhesion, P53 signaling, inflammation. ATACseq ChIPseq demonstrated as factor, primarily binds distal enhancers, influencing modifications without affecting proximal promoter regions. In addition, AR-induced maintained higher active states than AR-inhibited genes, even under ADT conditions. Furthermore, did not directly induce neuroendocrine differentiation cells, suggesting complex mechanism behind development. publicly available online application LNCaP-ADT ( https://pcatools.shinyapps.io/shinyADT/ ) was launched users visualize browse generated this study. Conclusion: This study provides comprehensive dataset, elucidating role at transcriptomic epigenomic levels. The reprocessed available, offering valuable resource future
Language: Английский
Citations
1
Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 3, 2024
Language: Английский
Citations
0