The follicular lymphoma and chronic lymphocytic leukemia proliferative microenvironment at single-cell resolution DOI Creative Commons
Antonio Ferreira, Shumei Wang, Larysa Poluben

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract Lymph nodes (LNs) are secondary lymphoid organs where lymphocytes interact with antigen presenting cells to initiate adaptive immune responses within microenvironments established by resident stromal cells. LNs also the major site of growth follicular lymphoma (FL) and chronic lymphocytic leukemia (CLL), B cell neoplasms that alter architecture in highly stereotypic ways. To characterize FL CLL LN microenvironment, we developed a pipeline for single-cell RNA sequencing all We observed proliferation specialized niches commences transient upregulation MYC, subsequent downregulation which may act limit potential these indolent neoplasms. Proliferating neoplastic follicles co-localized dendritic cells, whereas proliferating were spatially associated distinct set fibroblasts expressing CCL19 localized centers. used informatic analyses microscopy identify validate interacting sets ligand-receptor pairs between fibroblasts, including interactions involving CD74-MIF, TNFRSF13C (BAFF receptor), immunomodulatory factors such as CD55 Galectin-9 (Gal9), adhesion molecules. Our highlight common features two microenvironment-dependent provide roadmap identifying vulnerabilities new therapeutic strategies. Key points Nodal have similar proliferative programs predicted Proliferation occurs lymph node defined different types fibroblasts.

Language: Английский

Developing a risk score using liquid biopsy biomarkers for selecting Immunotherapy responders and stratifying disease progression risk in metastatic melanoma patients DOI Creative Commons
Amalia Azzariti, Simona De Summa, Tommaso Maria Marvulli

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: Feb. 5, 2025

Abstract Background Despite the high response rate to PD-1 blockade therapy in metastatic melanoma (MM) patients, a significant proportion of patients do not respond. Identifying biomarkers predict patient is crucial, ideally through non-invasive methods such as liquid biopsy. Methods Soluble forms PD1, PD-L1, LAG-3, CTLA-4, CD4, CD73, and CD74 were quantified using ELISA assay plasma cohort 110 MM at baseline, investigate possible correlations with clinical outcomes. A risk prediction model was applied validated pilot studies. Results No biomarker showed statistically differences between responders non-responders. However, number observed among certain Through univariate multivariate Cox analyses, we identified sPD-L1, sCTLA-4, sCD73, sCD74 independent predicting progression-free survival overall survival. According ROC analysis discovered that, except for values lower than cut-off predicted disease progression reduced mortality. comprehensive score developed by incorporating ​​of two factors, sCTLA-4 sCD74, which significantly improved accuracy outcome prediction. Pilot validations highlighted potential use treatment-naive individuals long responders. Conclusion In summary, based on circulating reflects immune checkpoint inhibitor (ICI) patients. If confirmed, further validation, these findings could assist recommending likely experience long-lasting response.

Language: Английский

Citations

1
IL-8 contributes to functional diversity of tumor-infiltrating neutrophils: A new target for cancer immunotherapy DOI
Ece Tavukçuoğlu, Güneş Esendağlı

Developmental Cell, Journal Year: 2025, Volume and Issue: 60(3), P. 339 - 341

Published: Feb. 1, 2025

Language: Английский

Citations

0
Tumor-associated stroma shapes the spatial tumor immune microenvironment of primary Ewing sarcomas DOI Creative Commons
Christopher Kuo, Krinio Giannikou,

N.-K. Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract To date, few studies have detailed the tumor microenvironment (TME) of Ewing sarcoma (EwS). The TME has a vital role in cancer survival and progression with implications drug resistance immune escape. By performing spatially resolved transcriptomic analysis primary treatment-naïve EwS samples, we discovered greater stromal enrichment localized tumors compared to metastatic tumors. Through spatial ligand-receptor analysis, show that enriched regions harbor unique extracellular matrix related cytokines, recruitment proinflammatory microenvironmental signals, implying stroma may play an anti-tumorigenic by acting as center. All expressed pro-tumorigenic MIF-CD74 signaling, suggesting potential immune-evasive mechanism immunotherapy target. Our findings provide insight into cell/stromal cell interactions serve valuable resource for further investigations EwS.

Language: Английский

Citations

0
Personalization of Cancer Treatment: Exploring the Role of Chronotherapy in Immune Checkpoint Inhibitor Efficacy DOI Open Access
Rosalyn M. Fey,

Avery Billo,

Terri Clister

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 732 - 732

Published: Feb. 21, 2025

In the era of precision medicine, mounting evidence suggests that time therapy administration, or chronotherapy, has a great impact on treatment outcomes. Chronotherapy involves planning timing by considering circadian rhythms, which are 24 h oscillations in behavior and physiology driven synchronized molecular clocks throughout body. The value chronotherapy cancer is currently under investigation, notably effects efficacy side effects. Immune checkpoint inhibitor (ICI) promising treatment. However, many patients still experience disease progression need to stop early due There accumulating day at ICI administered can have substantial effect efficacy. Thus, it important investigate intersections this review, we provide brief overview rhythms context immunity cancer. Additionally, outline current applications for We synthesize 29 studies conducted date examine time-of-day administration therapy, its associated effects, sex differences both also discuss potential mechanisms underlying these observed results. Finally, highlight challenges area future directions research, including chronotherapeutic personalized medicine approach tailors individual patients’ rhythms.

Language: Английский

Citations

0
Phase 2 trial of perioperative chemo-immunotherapy for gastro-esophageal adenocarcinoma: The role of M2 macrophage landscape in predicting response DOI Creative Commons
Thierry Alcindor, James Tankel, Pierre Fiset

et al.

Cell Reports Medicine, Journal Year: 2025, Volume and Issue: 6(4), P. 102045 - 102045

Published: April 1, 2025

Language: Английский

Citations

0
Serum Protein Profiling as theranostic biomarkers for Left- and Right-Sided Colon Cancer using Luminex ® technology DOI
Amani Attia,

Azza Habel,

Weili Xu

et al.

Cancer Biomarkers, Journal Year: 2025, Volume and Issue: 42(4)

Published: April 1, 2025

Background Given the differences between malignancies arising from different segments of colon, specific theranostic biomarkers can be linked to either Right-sided (RCC) or Left-sided colon cancer (LCC). Objective Analysis 65 serum proteins identify panels for LCC and RCC. Methods Serum levels immunomodulators were measured in CC, LCC, RCC patients, as well healthy controls with ProcartaPlex Human Immune Monitoring 65-Plex Panel. Results IL-27 may used early detection LCC. CD-30 was up-regulated metastatic BLC CD-40L down-regulated MDC MMP-1 positively associated, while IL-9 VEGF-A negatively associated lymph nodes invasion CC. Up-regulation IL-12p70 contrasted down-regulation MIP-1beta. IL-23, I-TAC, SDF-1α resistant CC Folfox chemotherapy, I-TAC IL-2 FGF-2 down-regulated, APRIL Conclusions Our study revealed significant protein emphasizing importance explore novel resistance sensitivity chemotherapy.

Language: Английский

Citations

0
Can tissue-specific PD-1/PD-L1 targeting combined with other immune checkpoint blockades enhance immune clearance? DOI
Sadia Ahmad,

Amna Rehman,

Ali Afzal

et al.

Medical Hypotheses, Journal Year: 2024, Volume and Issue: unknown, P. 111491 - 111491

Published: Sept. 1, 2024

Language: Английский

Citations

0
The follicular lymphoma and chronic lymphocytic leukemia proliferative microenvironment at single-cell resolution DOI Creative Commons
Antonio Ferreira, Shumei Wang, Larysa Poluben

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract Lymph nodes (LNs) are secondary lymphoid organs where lymphocytes interact with antigen presenting cells to initiate adaptive immune responses within microenvironments established by resident stromal cells. LNs also the major site of growth follicular lymphoma (FL) and chronic lymphocytic leukemia (CLL), B cell neoplasms that alter architecture in highly stereotypic ways. To characterize FL CLL LN microenvironment, we developed a pipeline for single-cell RNA sequencing all We observed proliferation specialized niches commences transient upregulation MYC, subsequent downregulation which may act limit potential these indolent neoplasms. Proliferating neoplastic follicles co-localized dendritic cells, whereas proliferating were spatially associated distinct set fibroblasts expressing CCL19 localized centers. used informatic analyses microscopy identify validate interacting sets ligand-receptor pairs between fibroblasts, including interactions involving CD74-MIF, TNFRSF13C (BAFF receptor), immunomodulatory factors such as CD55 Galectin-9 (Gal9), adhesion molecules. Our highlight common features two microenvironment-dependent provide roadmap identifying vulnerabilities new therapeutic strategies. Key points Nodal have similar proliferative programs predicted Proliferation occurs lymph node defined different types fibroblasts.

Language: Английский

Citations

0