Investigations of In Vitro Anti-Acetylcholinesterase, Anti-Diabetic, Anti-Inflammatory, and Anti-Cancer Efficacy of Garden Cress (Lepidium sativum Linn.) Seed Extracts, as Well as In Vivo Biochemical and Hematological Assays

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 446 - 446

Published: March 31, 2025

Background/Objectives: The current research was designed to quantify the active phyto-constituents and investigate in vitro biological efficiency of different garden cress (Lepidium sativum Linn.) seed extracts against chronic diseases as well vivo toxicities that may be induced mice upon administration each extract at both studied therapeutic doses. Methods: L. extracts, such methanolic, aqueous, acetone, ethyl acetate assessed. inhibition percentage (%) median inhibitory concentration (IC50) values were estimated acetylcholinesterase enzyme, diabetes mellitus (α-amylase α-glucosidase enzymes), inflammation (cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) enzymes). Additionally, HepG-2, Caco-2, A549 cells assessed using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Moreover, might hematological (using an automatic blood analyzer) biochemical levels evaluated. Results: It found methanolic possessed highest activities compared other extracts. 51.34, 54.35, 44.10, 43.48, 40.78% acetylcholinesterase, α-amylase, α-glucosidase, protein denaturation, proteinase enzymes, respectively. also exhibited effect COX-1 (55.05%), COX-2 (57.30%), 5-LOX (50.15%) enzymes. possesses cytotoxic activity cells, with IC50 52.27, 40.73, 37.95 μg/mL, lethal doses (LD50) showed safer when administered orally, followed by aqueous then extract. acetone no alterations orally two (1/10 1/20 LD50) control. Conclusions: This study concluded than In future, efficacy will evaluated, elucidation its mechanism diseases.

Language: Английский

Rita Levi-Montalcini: From Persecution to the Nobel Prize and an Honorary Degree From a Canadian University

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Rita Levi-Montalcini (RLM) is recognized as a prestigious and renowned researcher of her time. She was the fourth woman to earn Nobel Prize in Physiology Medicine 1986 for discovery nerve growth factor (NGF). We review biography scientific discovery, provide an example why still important. had special relationship with McGill University, Canada, which we describe. searched articles books about biographical material met Dr. Claudio Cuello, Former Chair McGill's Faculty Medicine. RLM born 1909 Turin, Italy, where she studied medicine. started career research. Because anti-Jewish racial laws Italy 1938, went underground continued projects bedroom. After war, visited St. Louis, USA, conducted research there. Her experiments confirmed that tumors release causes cancer proliferation. Initially, scientists responded this skepticism, but after its purification 1959 determination protein structure 1971, NGF became widely accepted. Currently, crosstalk between cancers nerves poorly understood. The prostate shows surgical or chemical denervation sympathetic prevents initiation tumors, whereas inhibition parasympathetic signaling reduces spread cancer. University awarded doctoral degree 2011. It first time history honorary doctorate outside second one Quebec. Through NGF, exemplified power passion despite obstacles faced. relentless dedication has led remarkable achievements.

Language: Английский