Exploration the Effects of Mesenchymal Stem Cells and Olive Leaf Extract on Physiological and Histopathological Changes on the Kidney of Diabetes Rats DOI Creative Commons

Mervat Ahmed AbdRabou,

Ahmed Atwa, Zahrah R Alrayes

et al.

Cellular Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 58(6), P. 807 - 822

Published: Dec. 28, 2024

Background/Aims: Gestational Diabetes Mellitus (GDM) is a common complication during pregnancy, defined as diabetes diagnosed in the second or third trimester, often asymptomatic. This study investigates therapeutic potential of olive leaf extracts and stem cells mitigating GDM-induced complications, particularly focusing on renal function, oxidative stress, pancreatic cell regeneration. Methods: Measurements were made gravid female rats with without intraperitoneal administration Streptozotocin (35 mg/kg body weight). Biochemical analyses conducted to evaluate function markers (urea, uric acid, creatinine) stress parameters (malondialdehyde, glutathione, superoxide dismutase levels). Histopathological immunohistopathological evaluations kidney tissues performed using hematoxylin eosin staining specific (p53, Insulin, PCNA) assess cellular changes. Results: The diabetic group exhibited significantly elevated levels urea, creatinine (p<0.01) compared control group. Treatment reduced these levels. Malondialdehyde but showed marked improvement treatment groups. Additionally, glutathione activities diminished (p<0.05) increased following treatment. revealed regeneration improved tissue morphology groups Conclusion: Stem exhibit significant ameliorating dysfunction, damage associated GDM, highlighting their role enhancing

Language: Английский

Exploration the Effects of Mesenchymal Stem Cells and Olive Leaf Extract on Physiological and Histopathological Changes on the Kidney of Diabetes Rats DOI Creative Commons

Mervat Ahmed AbdRabou,

Ahmed Atwa, Zahrah R Alrayes

et al.

Cellular Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 58(6), P. 807 - 822

Published: Dec. 28, 2024

Background/Aims: Gestational Diabetes Mellitus (GDM) is a common complication during pregnancy, defined as diabetes diagnosed in the second or third trimester, often asymptomatic. This study investigates therapeutic potential of olive leaf extracts and stem cells mitigating GDM-induced complications, particularly focusing on renal function, oxidative stress, pancreatic cell regeneration. Methods: Measurements were made gravid female rats with without intraperitoneal administration Streptozotocin (35 mg/kg body weight). Biochemical analyses conducted to evaluate function markers (urea, uric acid, creatinine) stress parameters (malondialdehyde, glutathione, superoxide dismutase levels). Histopathological immunohistopathological evaluations kidney tissues performed using hematoxylin eosin staining specific (p53, Insulin, PCNA) assess cellular changes. Results: The diabetic group exhibited significantly elevated levels urea, creatinine (p<0.01) compared control group. Treatment reduced these levels. Malondialdehyde but showed marked improvement treatment groups. Additionally, glutathione activities diminished (p<0.05) increased following treatment. revealed regeneration improved tissue morphology groups Conclusion: Stem exhibit significant ameliorating dysfunction, damage associated GDM, highlighting their role enhancing

Language: Английский

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