Advancing Radiobiology: Investigating the Effects of Photon, Proton, and Carbon-Ion Irradiation on PANC-1 Cells in 2D and 3D Tumor Models

Current Oncology, Journal Year: 2025, Volume and Issue: 32(1), P. 49 - 49

Published: Jan. 18, 2025

Introduction: Pancreatic cancer (PC) is one of the most aggressive and lethal malignancies, calling for enhanced research. ductal adenocarcinoma (PDAC) represents 70–80% all cases known its resistance to conventional therapies. Carbon-ion radiotherapy (CIRT) has emerged as a promising approach due ability deliver highly localized doses unique radiobiological properties compared X-rays. In vitro radiobiology relied on two-dimensional (2D) cell culture models so far; however, these are not sufficient replicate complexity in vivo tumor architecture. Three-dimensional (3D) become paradigm shift, surpassing constraints traditional by accurately re-creating morphological, histological, genetic characteristics well interaction tumour cells with microenvironment. Materials Methods: This study investigates survival pancreatic both 2D spheroids, 3D model, following photon, proton, carbon-ion irradiation means clonogenic, MTT, spheroid growth, vitality assays. Results: Our results demonstrate that carbon ions more efficient reducing photons protons. cultures, reduced approximately 15%, 45% 30% growth was similarly inhibited irradiation; overall rates were higher across modalities cultures. Carbon consistently showed highest efficacy viability models. Conclusions: research highlights pivotal role unraveling complexities radiobiology, offering new avenues designing effective precise treatment protocols.

Language: Английский

Investigating the Local Effectiveness of Carbon Ion Radiotherapy for Unresectable Female Genital Tract Melanomas: A Preliminary Real-World Study

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4147 - 4147

Published: Dec. 12, 2024

Background/Objectives: Primary gynecological melanomas are rare malignancies with lower survival rates compared to cutaneous melanomas. Both preclinical and clinical data support the evidence that mucosal photon-radioresistant but responsive carbon ion radiotherapy (CIRT). The aim of this study is assess, in a real-world cohort, effectiveness tolerability radical CIRT patients inoperable melanoma. Methods: This aimed assess safety setting. We defined as primary endpoints objective response rate (ORR) benefit (CB). secondary included actuarial local control (LC) assessed after 1 year 2 years toxicity scored according CTCAE v.5. Actuarial outcomes were analyzed using Kaplan–Meier method, while potential predictors investigated through Log-rank test. Results: Between 2017 2023, eleven Caucasian underwent pelvic (total dose 68.8 GyRBE) for malignant melanoma vulva or vagina. With median follow-up 18 months, we observed an ORR 82% CB 100%. LC at was 100% 86%, respectively, among factors their impact on LC, age < 60 seems be predictor (p = 0.014). treatment well tolerated, only one case acute grade 3 erythema and, late phase, urethral toxicity. Conclusions: effective safe Larger collaborative cohort studies longer follow-ups needed take step forward comprehending correct management disease.

Language: Английский

Radioresistant, Rare, Recurrent and Radioinduced: 4Rs of hadrontherapy for patients selections

International Journal of Particle Therapy, Journal Year: 2024, Volume and Issue: 15, P. 100737 - 100737

Published: Dec. 31, 2024

To describe the role of hadrontherapy (HT) in treating radioresistant, rare, recurrent, and radio-induced tumors, which can be defined, assonance with 4Rs radiobiology, "4Rs" HT indications. This is a narrative review written by multidisciplinary team consisting radiation oncologists, radiobiologists, physicists on current literature HT, particularly carbon ion therapy. refine indications within context framework, we evaluated tumor histologies across different clinical indication settings emphasized radiobiological mechanisms contributing to effectiveness HT. For has proven effective safe, achieving high rates local response mild toxicity. The shows that biological parameters assist clinicians identifying appropriate cases for treatment. Biological characteristics support administration tumors should considered during discussions.

Language: Английский