Exploring bacterial key genes and therapeutic agents for breast cancer among the Ghanaian female population: Insights from In Silico analyses DOI Creative Commons
Md. Kaderi Kibria, Md. Ahad Ali, Md. Nurul Haque Mollah

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0312493 - e0312493

Published: Nov. 25, 2024

Breast cancer (BC) is yet a significant global health challenge across various populations including Ghana, though several studies on host-genome associated with BC have been investigated molecular mechanisms of development and progression, candidate therapeutic agents. However, little attention has given microbial genome in this regard, although alterations microbiota epigenetic modifications are recognized as substantial risk factors for BC. This study focused identifying bacterial key genes (bKGs) infections the Ghanaian population exploring potential drug molecules by targeting these bKGs through silico analyses. At first, 16S rRNA sequence data were downloaded from NCBI database comprising 520 samples patients 442 healthy controls. Analysis rRNA-Seq showed differences abundance between groups identified 26 differential genera threshold values at |log2FC|>2.0 p-value≤0.05. It was observed that two Prevotella Anaerovibria significantly upregulated others downregulated. Functional analysis based all 19 MetaCyc signaling pathways, twelve which enriched containing 165 Top-ranked 10 mdh, pykF, gapA, zwf, pgi, tpiA, pgk, pfkA, ppsA, pykA BC-causing protein-protein interaction network analysis. Subsequently, bKG-guided top ranked Digitoxin, Digoxin, Ledipasvir, Suramin, Ergotamine, Venetoclax, Nilotinib, Conivaptan, Dihydroergotamine, Elbasvir using docking The stability top-ranked three drug-target complexes (Digitoxin-pykA, Digoxin-mdh, Ledipasvir-pgi) confirmed dynamics simulation studies. Therefore, findings might be useful resources to wet-lab researchers further experimental validation therapies against

Language: Английский

The Role of the Microbiome and of Radiotherapy-Derived Metabolites in Breast Cancer DOI Open Access
Lourdes Herrera‐Quintana, Héctor Vázquez‐Lorente, Rafael Cardoso Maciel Costa Silva

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(21), P. 3671 - 3671

Published: Oct. 30, 2024

The gut microbiome has emerged as a crucial player in modulating cancer therapies, including radiotherapy. In the case of breast cancer, interplay between and radiotherapy-derived metabolites may enhance therapeutic outcomes minimize adverse effects. this review, we explore bidirectional relationship cancer. We explain how composition influences progression treatment response, its treatments influence composition. A dual role for is explored article, highlighting both their benefits potential hazards. By integrating genomics, metabolomics, bioinformatics tools, present comprehensive overview these interactions. study provides real-world insight through studies clinical trials, while innovations such probiotics, dietary interventions are examined to modulate effectiveness. Moreover, ethical considerations patient perspectives discussed, ensuring understanding subject. Towards revolutionizing strategies improving outcomes, review concludes with future research directions. It also envisions metabolite into personalized therapy.

Language: Английский

Citations

3
Exploring bacterial key genes and therapeutic agents for breast cancer among the Ghanaian female population: Insights from In Silico analyses DOI Creative Commons
Md. Kaderi Kibria, Md. Ahad Ali, Md. Nurul Haque Mollah

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0312493 - e0312493

Published: Nov. 25, 2024

Breast cancer (BC) is yet a significant global health challenge across various populations including Ghana, though several studies on host-genome associated with BC have been investigated molecular mechanisms of development and progression, candidate therapeutic agents. However, little attention has given microbial genome in this regard, although alterations microbiota epigenetic modifications are recognized as substantial risk factors for BC. This study focused identifying bacterial key genes (bKGs) infections the Ghanaian population exploring potential drug molecules by targeting these bKGs through silico analyses. At first, 16S rRNA sequence data were downloaded from NCBI database comprising 520 samples patients 442 healthy controls. Analysis rRNA-Seq showed differences abundance between groups identified 26 differential genera threshold values at |log2FC|>2.0 p-value≤0.05. It was observed that two Prevotella Anaerovibria significantly upregulated others downregulated. Functional analysis based all 19 MetaCyc signaling pathways, twelve which enriched containing 165 Top-ranked 10 mdh, pykF, gapA, zwf, pgi, tpiA, pgk, pfkA, ppsA, pykA BC-causing protein-protein interaction network analysis. Subsequently, bKG-guided top ranked Digitoxin, Digoxin, Ledipasvir, Suramin, Ergotamine, Venetoclax, Nilotinib, Conivaptan, Dihydroergotamine, Elbasvir using docking The stability top-ranked three drug-target complexes (Digitoxin-pykA, Digoxin-mdh, Ledipasvir-pgi) confirmed dynamics simulation studies. Therefore, findings might be useful resources to wet-lab researchers further experimental validation therapies against

Language: Английский

Citations

1