Identifcation of the FGF family as therapeutic targets and prognostic biomarkers in the microenvironment of head and neck squamous cell carcinoma DOI Creative Commons
Li Zhang, Yingchun Gao, Yumei Tian

et al.

SLAS TECHNOLOGY, Journal Year: 2025, Volume and Issue: unknown, P. 100271 - 100271

Published: March 1, 2025

Almost 90% of head and neck malignancies are malignant squamous cell cancers, making it the sixth most common malignancy in developing countries, with an overall five-year survival rate about 40%-50%. Early diagnosis treatment can bring a better prognosis. Fibroblast growth factor (FGF) is important polypeptide vivo. Studies have found that FGF signal has carcinogenic potential participates variety behaviors. Some experiments proved function tumor inhibition some cases, role signalling tissue repair homeostasis suggest for targeted therapy However, its manifestation predictive HNSC not been clearly defined. Genome-wide expression analysis Oncomine evaluated evaluation family HNSC. Expression data set were used to obtain T statistic was applied analysis. The differential mRNA levels versus normal tissues, as well correlation pathological staging prognosis, examined using GEPIA single-gene tool family.FGF altered CO network modules obtained from cBioportal analyzed 520 samples.Pro-protein interaction (PPI) flow performed on differentially ordered clusters STRING, Gene Operating System (GO) domain enrichment Kyoto Encyclopedia Genes Genomes (KEGG) pathway cluster neighbouring genes DAVID6.8, key transcriptional factors (TF) by TRRUST, between level autoimmune migration TIMER, biological kinase target LinkInterpreter. Only FGF6 down-regulated all family(FC=2),Transcriptional FGF1, FGF2, FGF5, FGF7-14, FGF17-19, FGF21 FGF22 upregulated .In terms relative HNSC, greatest amount FGF11. In different stages meaningless (P>0.05), FGF3-6, FGF8-10, FGF14, FGF16, FGF17, FGF19 -21, FGF23 showed no significant difference stages. Low FGF5 high had low survival(OS) HNSC(P =0.012, P =0.0015). addition, highly abundant PI3K-Akt signaling pathway, MAPK rasper pathway. Our ATF4, STAT, RELA, NFKB1 transcription family, NLK, LOCK1, LYN, ZAP70, MAP2K3, RPS6KA4, AURKB, ATR, ROCK1, MYLK2, CAMK2A, EGFR, MAPK3, MAP3K8, SYK, LCK, HCK, PKN2, RPS6KA1, BUB1, CDK5, ITK, FYN, TBK1, ATM, CDK2, PTK2 targets family. We identified relationship modulation cellular infiltration, such B lymphocytes, CD4+ cells macrophages dendritic cells. may shed new light choice immunotherapeutic biomarkers

Language: Английский

Identifcation of the FGF family as therapeutic targets and prognostic biomarkers in the microenvironment of head and neck squamous cell carcinoma DOI Creative Commons
Li Zhang, Yingchun Gao, Yumei Tian

et al.

SLAS TECHNOLOGY, Journal Year: 2025, Volume and Issue: unknown, P. 100271 - 100271

Published: March 1, 2025

Almost 90% of head and neck malignancies are malignant squamous cell cancers, making it the sixth most common malignancy in developing countries, with an overall five-year survival rate about 40%-50%. Early diagnosis treatment can bring a better prognosis. Fibroblast growth factor (FGF) is important polypeptide vivo. Studies have found that FGF signal has carcinogenic potential participates variety behaviors. Some experiments proved function tumor inhibition some cases, role signalling tissue repair homeostasis suggest for targeted therapy However, its manifestation predictive HNSC not been clearly defined. Genome-wide expression analysis Oncomine evaluated evaluation family HNSC. Expression data set were used to obtain T statistic was applied analysis. The differential mRNA levels versus normal tissues, as well correlation pathological staging prognosis, examined using GEPIA single-gene tool family.FGF altered CO network modules obtained from cBioportal analyzed 520 samples.Pro-protein interaction (PPI) flow performed on differentially ordered clusters STRING, Gene Operating System (GO) domain enrichment Kyoto Encyclopedia Genes Genomes (KEGG) pathway cluster neighbouring genes DAVID6.8, key transcriptional factors (TF) by TRRUST, between level autoimmune migration TIMER, biological kinase target LinkInterpreter. Only FGF6 down-regulated all family(FC=2),Transcriptional FGF1, FGF2, FGF5, FGF7-14, FGF17-19, FGF21 FGF22 upregulated .In terms relative HNSC, greatest amount FGF11. In different stages meaningless (P>0.05), FGF3-6, FGF8-10, FGF14, FGF16, FGF17, FGF19 -21, FGF23 showed no significant difference stages. Low FGF5 high had low survival(OS) HNSC(P =0.012, P =0.0015). addition, highly abundant PI3K-Akt signaling pathway, MAPK rasper pathway. Our ATF4, STAT, RELA, NFKB1 transcription family, NLK, LOCK1, LYN, ZAP70, MAP2K3, RPS6KA4, AURKB, ATR, ROCK1, MYLK2, CAMK2A, EGFR, MAPK3, MAP3K8, SYK, LCK, HCK, PKN2, RPS6KA1, BUB1, CDK5, ITK, FYN, TBK1, ATM, CDK2, PTK2 targets family. We identified relationship modulation cellular infiltration, such B lymphocytes, CD4+ cells macrophages dendritic cells. may shed new light choice immunotherapeutic biomarkers

Language: Английский

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