Preparation of Iron oxide/palladium nanoparticles modified with carbon quantum dots (Pd@CQD@Fe3O4): insight to anti-cancer effect and ROS generation DOI Creative Commons

Parnian Rajabzadeh,

Pooneh Zoorazma,

Maryam Abbasi

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 19, 2025

Advances in nanoscience and nanotechnology have facilitated the application of nanoparticles various biomedical fields. This study synthesized iron oxide/palladium modified with carbon quantum dots (Pd@CQD@Fe₃O₄) evaluated their cytotoxic anticancer effects on SK-OV-3 (ovarian carcinoma) MCF-7 (breast adenocarcinoma) human cell lines. Characterization Pd@CQD@Fe₃O₄ was performed using SEM, EDS, XPS, VSM zeta potential analysis. The Pd@CQD@Fe₃O₄-treated group exhibited a significantly increased generation reactive oxygen species (ROS) compared to control group. To assess activities nanoparticles, MTT assay utilized, revealing dose- time-dependent response. Flow cytometry-based apoptosis assays revealed that cytotoxicity mediated through both cells. Gene expression analysis utilizing real-time PCR significant alterations Bax, Bcl-2, caspase-8, caspase-9 levels following treatment. Notably, nanoparticle higher against SK-OV-3, cancerous cells normal Caspase-8 mRNA by 1.40-fold 1.11-fold Caspase-3/7 protein rose 3.00-fold induced total 79.8% 77.01% cells, as confirmed flow cytometry, indicating strong pro-apoptotic activity These findings suggest possess potent offer for targeted drug delivery enhancement.

Language: Английский

Preparation of Iron oxide/palladium nanoparticles modified with carbon quantum dots (Pd@CQD@Fe3O4): insight to anti-cancer effect and ROS generation DOI Creative Commons

Parnian Rajabzadeh,

Pooneh Zoorazma,

Maryam Abbasi

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 19, 2025

Advances in nanoscience and nanotechnology have facilitated the application of nanoparticles various biomedical fields. This study synthesized iron oxide/palladium modified with carbon quantum dots (Pd@CQD@Fe₃O₄) evaluated their cytotoxic anticancer effects on SK-OV-3 (ovarian carcinoma) MCF-7 (breast adenocarcinoma) human cell lines. Characterization Pd@CQD@Fe₃O₄ was performed using SEM, EDS, XPS, VSM zeta potential analysis. The Pd@CQD@Fe₃O₄-treated group exhibited a significantly increased generation reactive oxygen species (ROS) compared to control group. To assess activities nanoparticles, MTT assay utilized, revealing dose- time-dependent response. Flow cytometry-based apoptosis assays revealed that cytotoxicity mediated through both cells. Gene expression analysis utilizing real-time PCR significant alterations Bax, Bcl-2, caspase-8, caspase-9 levels following treatment. Notably, nanoparticle higher against SK-OV-3, cancerous cells normal Caspase-8 mRNA by 1.40-fold 1.11-fold Caspase-3/7 protein rose 3.00-fold induced total 79.8% 77.01% cells, as confirmed flow cytometry, indicating strong pro-apoptotic activity These findings suggest possess potent offer for targeted drug delivery enhancement.

Language: Английский

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