KPNA5 Suppresses Malignant Progression of Ovarian Cancer Through Importing the PTPN4 Into the Nucleus DOI Creative Commons

Yanming Hu,

Jin Zhou, Xinru Ling

et al.

Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(7)

Published: March 27, 2025

ABSTRACT Background Abnormal protein localization due to disrupted nucleoplasmic transport is common in tumor cells, but its mechanisms are not well understood. Nuclear pore complexes and nuclear transporter proteins crucial for between the nucleus cytoplasm. Evidence increasingly shows that abnormal expression of karyopherin family disrupts translocation, affecting processes like cell differentiation, proliferation, apoptosis, transcriptional regulation. However, their functions roles ovarian cancer remain unclear. Methods The level KPNA5 tissues cells was detected by IHC, Western blot, qPCR. CCK‐8 colony formation assays were used assess proliferation ability. Transwell assay conducted determine migration invasion capacity. A xenograft model effect on growth vivo. Results downregulated (OC) tissues. Low levels associated with poor survival OC patients, validated an tissue sample cohort. Overexpression significantly suppressed growth, both vitro vivo studies. Mechanistically, recognizes signals (NLSs) PTPN4, mediating inhibiting STAT3 phosphorylation downstream signaling pathway. Similarly, PTPN4 overexpression reduced viability invasion, also suppressing phosphorylation. Conclusions Our findings identify as a suppressor OC, presenting potential therapeutic target treatment.

Language: Английский

KPNA5 Suppresses Malignant Progression of Ovarian Cancer Through Importing the PTPN4 Into the Nucleus DOI Creative Commons

Yanming Hu,

Jin Zhou, Xinru Ling

et al.

Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(7)

Published: March 27, 2025

ABSTRACT Background Abnormal protein localization due to disrupted nucleoplasmic transport is common in tumor cells, but its mechanisms are not well understood. Nuclear pore complexes and nuclear transporter proteins crucial for between the nucleus cytoplasm. Evidence increasingly shows that abnormal expression of karyopherin family disrupts translocation, affecting processes like cell differentiation, proliferation, apoptosis, transcriptional regulation. However, their functions roles ovarian cancer remain unclear. Methods The level KPNA5 tissues cells was detected by IHC, Western blot, qPCR. CCK‐8 colony formation assays were used assess proliferation ability. Transwell assay conducted determine migration invasion capacity. A xenograft model effect on growth vivo. Results downregulated (OC) tissues. Low levels associated with poor survival OC patients, validated an tissue sample cohort. Overexpression significantly suppressed growth, both vitro vivo studies. Mechanistically, recognizes signals (NLSs) PTPN4, mediating inhibiting STAT3 phosphorylation downstream signaling pathway. Similarly, PTPN4 overexpression reduced viability invasion, also suppressing phosphorylation. Conclusions Our findings identify as a suppressor OC, presenting potential therapeutic target treatment.

Language: Английский

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