Efficient hydroxylation of flavonoids by using whole-cell P450 sca-2 biocatalyst in Escherichia coli DOI Creative Commons

Baodong Hu,

Xinrui Zhao, Jingwen Zhou

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 15, 2023

The hydroxylation is an important way to generate the functionalized derivatives of flavonoids. However, efficient flavonoids by bacterial P450 enzymes rarely reported. Here, a sca-2 mut whole-cell biocatalyst with outstanding 3′-hydroxylation activity for variety was first enhanced using novel combination flavodoxin Fld and reductase Fpr from Escherichia coli. In addition, double mutant (R88A/S96A) exhibited improved performance through enzymatic engineering. Moreover, further optimization biocatalytic conditions. Finally, eriodictyol, dihydroquercetin, luteolin, 7,3′,4′-trihydroxyisoflavone, as examples flavanone, flavanonol, flavone, isoflavone, were produced biocatalysis naringenin, dihydrokaempferol, apigenin, daidzein substrates, conversion yield 77%, 66%, 32%, 75%, respectively. strategy used in this study provided effective method other high value-added compounds.

Language: Английский

Harnessing Aromatic Properties for Sustainable Bio-valorization of Lignin Derivatives into Flavonoids DOI Creative Commons
Siyu Zhu, Na Li, Zhihua Liu

et al.

Green Carbon, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Engineering Saccharomyces cerevisiae for De Novo Biosynthesis of 3′-Hydroxygenistein DOI

Xinjia Tan,

Zhiqiang Xiao, Siqi Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

The polyhydroxy isoflavone 3′-hydroxygenistein (3′-OHG) has a wide range of pharmaceutical and nutraceutical benefits. Therefore, it is important to develop an efficient sustainable method for 3′-OHG production. Here, we engineered the metabolic pathways Saccharomyces cerevisiae achieve de novo biosynthesis 3′-OHG. First, screened 2-hydroxyisoflavanone synthase (IFS), cytochrome P450 reductase, dehydratase from different sources optimized best combination via promoter engineering. Next, demonstrated that amplification rate-limiting enzyme PlIFS Pueraria lobata improved genistein Increasing availability cofactor heme further increased titer 44.55 ± 1.82 mg/L. Subsequently, screening multicopy integration isoflavone-3′-hydroxylase achieved 13.23 0.27 mg/L 100 naringenin. Finally, 1.40 0.02 could be pathway. final strain generated in this study will facilitate production isoflavones biosynthetic

Language: Английский

Citations

0
Efficient hydroxylation of flavonoids by using whole-cell P450 sca-2 biocatalyst in Escherichia coli DOI Creative Commons

Baodong Hu,

Xinrui Zhao, Jingwen Zhou

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 15, 2023

The hydroxylation is an important way to generate the functionalized derivatives of flavonoids. However, efficient flavonoids by bacterial P450 enzymes rarely reported. Here, a sca-2 mut whole-cell biocatalyst with outstanding 3′-hydroxylation activity for variety was first enhanced using novel combination flavodoxin Fld and reductase Fpr from Escherichia coli. In addition, double mutant (R88A/S96A) exhibited improved performance through enzymatic engineering. Moreover, further optimization biocatalytic conditions. Finally, eriodictyol, dihydroquercetin, luteolin, 7,3′,4′-trihydroxyisoflavone, as examples flavanone, flavanonol, flavone, isoflavone, were produced biocatalysis naringenin, dihydrokaempferol, apigenin, daidzein substrates, conversion yield 77%, 66%, 32%, 75%, respectively. strategy used in this study provided effective method other high value-added compounds.

Language: Английский

Citations

6