Fermentation,
Journal Year:
2024,
Volume and Issue:
11(1), P. 3 - 3
Published: Dec. 24, 2024
This
study
investigates
the
potential
of
sequential
solid-state
and
submerged
fermentation
(SeqF)
to
enhance
lipase
production
by
Yarrowia
lipolytica
using
by-products
from
palm
oil
chain.
Palm
fiber
deodorizer
distillate
(PODD)
were
utilized
as
substrates
in
both
stages.
Solid-state
(SSF)
yielded
significant
activity
when
was
used
alone
(1.55
U/g
48
h),
while
(SmF)
showed
improved
enzymatic
with
combination
PODD
(1171
U/L
72
h).
The
integration
SSF
SmF
SeqF
achieved
superior
activities,
reaching
4464.5
U/L,
an
8.3-fold
increase
compared
alone,
Erlenmeyer
flasks.
SeqF-lyophilized
biocatalysts
experiments
better
hydrolytic
(131
U/g)
best
conditions
reproduced
a
4
L
bioreactor
(33
U/g).
biocatalyst
employed
esterification
reactions
synthesize
mono-
diacylglycerols,
achieving
24.3%
conversion
rate.
highlights
promising
sustainable
approach
for
valorizing
agro-industrial
residues,
contributing
advancing
circular
bioeconomy
initiatives.
Fermentation,
Journal Year:
2023,
Volume and Issue:
9(11), P. 989 - 989
Published: Nov. 20, 2023
Lipases
are
enzymes
that
catalyze
the
hydrolysis
of
ester
bonds
triacylglycerols
at
oil–water
interface,
generating
free
fatty
acids,
glycerol,
diacylglycerol,
and
monoacylglycerol,
which
can
be
produced
from
fermentation
agro-industrial
by-products
rich
in
such
as
cupuaçu
fat
cake.
In
this
study,
Yarrowia
lipolytica
IMUFRJ50682
was
used
for
lipase
production
cake
solid-state
(SSF)
associated
with
soybean
meal.
The
2:1
ratio
cake/soybean
meal
increased
activity
Y.
via
SSF
by
approximately
30.3-fold
compared
to
without
supplementation.
optimal
conditions
produce
were
obtained
supplementation
peptone,
urea,
oil
(all
1.5%
w/v),
reaching
values
up
70.6
U
g−1.
These
results
demonstrate
adds
value
by-products.
Furthermore,
proper
processing
contribute
improving
economic
viability
biotechnological
industry
help
prevent
accumulation
waste
environmental
pollution.
Fermentation,
Journal Year:
2024,
Volume and Issue:
11(1), P. 3 - 3
Published: Dec. 24, 2024
This
study
investigates
the
potential
of
sequential
solid-state
and
submerged
fermentation
(SeqF)
to
enhance
lipase
production
by
Yarrowia
lipolytica
using
by-products
from
palm
oil
chain.
Palm
fiber
deodorizer
distillate
(PODD)
were
utilized
as
substrates
in
both
stages.
Solid-state
(SSF)
yielded
significant
activity
when
was
used
alone
(1.55
U/g
48
h),
while
(SmF)
showed
improved
enzymatic
with
combination
PODD
(1171
U/L
72
h).
The
integration
SSF
SmF
SeqF
achieved
superior
activities,
reaching
4464.5
U/L,
an
8.3-fold
increase
compared
alone,
Erlenmeyer
flasks.
SeqF-lyophilized
biocatalysts
experiments
better
hydrolytic
(131
U/g)
best
conditions
reproduced
a
4
L
bioreactor
(33
U/g).
biocatalyst
employed
esterification
reactions
synthesize
mono-
diacylglycerols,
achieving
24.3%
conversion
rate.
highlights
promising
sustainable
approach
for
valorizing
agro-industrial
residues,
contributing
advancing
circular
bioeconomy
initiatives.
