RNA Modifications and Their Role in Regulating KSHV Replication and Pathogenic Mechanisms DOI Open Access

S. Schultz,

K. Gomard‐Henshaw,

Mandy Muller

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 97(1)

Published: Dec. 30, 2024

Kaposi's sarcoma-associated herpesvirus is an oncogenic gammaherpesvirus that plays a major role in several human malignancies, including sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. The complexity of KSHV biology reflected the sophisticated regulation its biphasic life cycle, consisting quiescent latent phase virion-producing lytic replication. expresses coding noncoding RNAs, microRNAs long which play crucial roles modulating viral gene expression, immune evasion, intercellular communication. Recent studies have highlighted importance RNA modifications, also known as epitranscriptome, regulating KSHV-encoded adding novel layer posttranscriptional control previously unknown. These such N

Language: Английский

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells DOI Creative Commons
Lu Chen, Wenjing Wang, Qiang Liu

et al.

Nucleic Acids Research, Journal Year: 2022, Volume and Issue: 50(19), P. 10896 - 10913

Published: July 8, 2022

Post-transcriptional RNA modifications critically regulate various biological processes. N4-acetylcytidine (ac4C) is an epi-transcriptome, which highly conserved in all species. However, the vivo physiological functions and regulatory mechanisms of ac4C remain poorly understood, particularly mammals. In this study, we demonstrate that only known writer, N-acetyltransferase 10 (NAT10), plays essential role male reproduction. We identified occurrence mRNAs mouse tissues showed undergoes dynamic changes during spermatogenesis. Germ cell-specific ablation Nat10 severely inhibits meiotic entry leads to defects homologous chromosome synapsis, recombination repair DNA double-strand breaks meiosis. Transcriptomic profiling revealed dysregulation functional genes prophase I after deletion. These findings highlight crucial spermatogenesis expand our understanding its regulation specific processes vivo.

Language: Английский

Citations

53
Emerging role of RNA acetylation modification ac4C in diseases: Current advances and future challenges DOI
Jie Luo, Jingsong Cao, Cong Chen

et al.

Biochemical Pharmacology, Journal Year: 2023, Volume and Issue: 213, P. 115628 - 115628

Published: May 27, 2023

Language: Английский

Citations

34
Plant YTHDF proteins are direct effectors of antiviral immunity against an N6‐methyladenosine‐containing RNA virus DOI Creative Commons
Mireya Martínez‐Pérez, Frederic Aparicio, Laura Arribas‐Hernández

et al.

The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(18)

Published: July 11, 2023

In virus-host interactions, nucleic acid-directed first lines of defense that allow viral clearance without compromising growth are paramount importance. Plants use the RNA interference pathway as a basal antiviral immune system, but additional RNA-based mechanisms also exist. The infectivity plant positive-strand virus, alfalfa mosaic virus (AMV), relies on demethylation by recruitment cellular N6-methyladenosine (m

Language: Английский

Citations

23
Noncoding RNAs in the COVID-19 Saga: An Untold Story DOI
Mudasir Maqbool, Md Sadique Hussain, Nusrat K. Shaikh

et al.

Viral Immunology, Journal Year: 2024, Volume and Issue: 37(6), P. 269 - 286

Published: July 5, 2024

Language: Английский

Citations

9
N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71 DOI

Haojie Hao,

Weichi Liu,

Yuanjiu Miao

et al.

Nucleic Acids Research, Journal Year: 2022, Volume and Issue: 50(16), P. 9339 - 9354

Published: Aug. 16, 2022

Abstract Chemical modifications are important for RNA function and metabolism. N4-acetylcytidine (ac4C) is critical the translation stability of mRNA. Although ac4C found in viruses, detailed mechanisms through which affects viral replication unclear. Here, we reported that 5′ untranslated region enterovirus 71 (EV71) genome was modified by host acetyltransferase NAT10. Inhibition NAT10 mutation sites within internal ribosomal entry site (IRES) suppressed EV71 replication. enhanced via selective recruitment PCBP2 to IRES boosted stability. Additionally, increased binding RNA-dependent polymerase (3D) RNA. Notably, ac4C-deficient mutant showed reduced pathogenicity vivo. Our findings highlighted essential role infection provided insights into potential antiviral treatments.

Language: Английский

Citations

38
ISG20: an enigmatic antiviral RNase targeting multiple viruses DOI Creative Commons
Séverine Deymier, Camille Louvat, Francesca Fiorini

et al.

FEBS Open Bio, Journal Year: 2022, Volume and Issue: 12(6), P. 1096 - 1111

Published: Feb. 17, 2022

Interferon‐stimulated gene 20 kDa protein (ISG20) is a relatively understudied antiviral capable of inhibiting broad spectrum viruses. ISG20 exhibits strong RNase properties, and it belongs to the large family DEDD exonucleases, present in both prokaryotes eukaryotes. was initially characterized as having activity vitro , suggesting that its inhibitory effects are mediated via direct degradation viral RNAs. This mechanism action has since been further elucidated additional activities highlighted, including deaminated DNA translational inhibition RNA nonself review focuses on current understanding main molecular mechanisms by discusses latest developments features govern specificity or resistance action.

Language: Английский

Citations

37
NSUN2-mediated m5C modification of HBV RNA positively regulates HBV replication DOI Creative Commons
Jiangpeng Feng,

Tianmo Xu,

Miao He

et al.

PLoS Pathogens, Journal Year: 2023, Volume and Issue: 19(12), P. e1011808 - e1011808

Published: Dec. 4, 2023

Chronic hepatitis B virus (HBV) infection is a major cause of liver cirrhosis and cancer, despite strong prevention treatment efforts. The study the epigenetic modification HBV has become research hotspot, including N 6-methyladenosine (m 6 A) RNA, which plays complex roles in life cycle. In addition to m A modification, 5-methylcytosine 5 C) another eukaryotic mRNA. this study, we explored C methyltransferase demethyltransferase results showed that NSUN2 deficiency could negatively regulate expression while TET2 positively regulates HBV. Subsequently, combined both vitro bisulfite sequencing high-throughput methods determine distribution stoichiometry RNA. Two sites: C2017 C131 with highest-ranking methylation rates were identified, mutations at these two sites lead decreased replication HBV, mutation “fake” site had no effect. Mechanistically, NSUN2-mediated promotes stability addition, compared wild-type HepG2-NTCP cells primary human hepatocytes, level after knockdown decreased, ability mutant infect replicate PHHs was substantially impaired. Similar found experiments using C57BL/6JGpt- Nsun2 +/- mice. Interestingly, also core protein promoted endogenous NSUN2, implied positive feedback loop. summary, our provides an accurate high-resolution profile RNA reveals by maintaining stability.

Language: Английский

Citations

18
The Applications of Nanopore Sequencing Technology in Animal and Human Virus Research DOI Creative Commons

Chun-Miao Ji,

Xiao-Yin Feng,

Yao‐Wei Huang

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 798 - 798

Published: May 16, 2024

In recent years, an increasing number of viruses have triggered outbreaks that pose a severe threat to both human and animal life, as well caused substantial economic losses. It is crucial understand the genomic structure epidemiology these guide effective clinical prevention treatment strategies. Nanopore sequencing, third-generation sequencing technology, has been widely used in research since 2014. This technology offers several advantages over traditional methods next-generation (NGS), such ability generate ultra-long reads, high efficiency, real-time monitoring analysis, portability, directly sequence RNA or DNA molecules. As result, it exhibits excellent applicability flexibility virus research, including viral detection surveillance, genome assembly, discovery new variants novel viruses, identification chemical modifications. this paper, we provide comprehensive review development, principles, advantages, applications nanopore aiming offer fresh perspectives for future studies field.

Language: Английский

Citations

6
The Emerging Role of RNA Modifications in the Regulation of Antiviral Innate Immunity DOI Creative Commons
Jie Tong,

Wuchao Zhang,

Yuran Chen

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Feb. 3, 2022

Posttranscriptional modifications have been implicated in regulation of nearly all biological aspects cellular RNAs, from stability, translation, splicing, nuclear export to localization. Chemical also revealed for virus derived RNAs several decades before, along with the potential their regulatory roles infection. Due dynamic changes RNA during infection, illustrating mechanisms epigenetic regulations remains a challenge. Nevertheless, many studies indicated that these marks may directly regulate infection through antiviral innate immune responses. The present review summarizes impacts important on viral including N6-methyladenosine (m6A), 5-methylcytidine (m5C), 2'-O-methylation (2'-O-Methyl), and few uncanonical nucleotides (A-to-I editing, pseudouridine), immunity relevant signaling pathways, while highlighting significance responses

Language: Английский

Citations

28
Nanopore-Based Detection of Viral RNA Modifications DOI
Jonathan S. Abebe, Ruth Verstraten, Daniel P. Depledge

et al.

mBio, Journal Year: 2022, Volume and Issue: 13(3)

Published: May 17, 2022

The chemical modification of ribonucleotides plays an integral role in the biology diverse viruses and their eukaryotic host cells. Mapping precise identity, location, abundance modified remains a key goal many studies aimed at characterizing function importance given modification.

Language: Английский

Citations

24