The Drosophila drop-dead gene is required for eggshell integrity DOI Creative Commons
Tayler D. Sheahan,

Amanpreet Grewal,

Laura E. Korthauer

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(12), P. e0295412 - e0295412

Published: Dec. 5, 2023

The eggshell of the fruit fly Drosophila melanogaster is a useful model for understanding synthesis complex extracellular matrix. synthesized during mid-to-late oogenesis by somatic follicle cells that surround developing oocyte. We previously reported female flies mutant gene drop-dead (drd) are sterile, but underlying cause sterility remained unknown. In this study, we examined role drd in synthesis. show eggs laid females fertilized arrest early embryogenesis, and innermost layer eggshell, vitelline membrane, abnormally permeable to dye these eggs. addition, major membrane proteins fail become crosslinked nonreducible bonds, process normally occurs egg activation following ovulation, as evidenced their solubility detection Western blot contrast, Cp36 protein, which found outer chorion layers becomes normally. To link expression pattern with phenotypes, expressed ovarian beginning mid-oogenesis, and, importantly, all phenotypes could be recapitulated selective knockdown cells. determine whether was required crosslinking itself, performed vitro experiments. membranes control chambers either incubation hyperosmotic medium, activates chambers, or exogenous peroxidase hydrogen peroxide. neither treatment resulted chambers. These results indicate necessary serve substrates peroxidase-mediated cross-linking at end oogenesis.

Language: Английский

Testing the Protective Properties of the Extract of Monarda Fistulosa Var. Menthifolia L. Regarding the Effect of the Anticancer Drug Etoposide on the Example of Drosophila Melanogaster DOI
O. N. Antosyuk, Е. В. Болотник,

D. V. Obvitsev

et al.

Journal of Herbs Spices & Medicinal Plants, Journal Year: 2023, Volume and Issue: 29(3), P. 308 - 317

Published: Feb. 20, 2023

The protective properties of the extract Monarda fistulosa var. menthifolia were tested when used together with antitumor drug etoposide on Drosophila melanogaster. 1%, 10% extracts M. tested, separately and at 800 µg kg−1 nutrient medium. genotoxic effect was determined using SMART method. Cytotoxicity by assessing regulated cell death (RCD). While 1% (20 mg mL−1) did not show genotoxicity, had antigenotoxicity, reducing genetic activity anticancer etoposide, (200 it showed geno- cytotoxicity.

Language: Английский

Citations

1
An evolutionary mechanism to assimilate new nutrient sensors into the mTORC1 pathway DOI Creative Commons
Grace Y. Liu, Patrick Jouandin,

Raymond E. Bahng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 26, 2023

Animals must sense and respond to nutrient availability in their local niche. This task is coordinated part by the mTOR complex 1 (mTORC1) pathway, which regulates growth metabolism response nutrients1-5. In mammals, mTORC1 senses specific amino acids through specialized sensors that act upstream GATOR1/2 signaling hub6-8. To reconcile conserved architecture of pathway with diversity environments animals can occupy, we hypothesized might maintain plasticity evolving distinct different metazoan phyla1,9,10. Whether such customization occurs-and how capture new inputs-is not known. Here, identify Drosophila melanogaster protein Unmet expectations (Unmet, formerly CG11596) as a species-restricted sensor trace its incorporation into pathway. Upon methionine starvation, binds fly GATOR2 inhibit dTORC1. S-adenosylmethionine (SAM), proxy for availability, directly relieves this inhibition. expression elevated ovary, methionine-sensitive niche11, flies lacking fail integrity female germline under restriction. By monitoring evolutionary history Unmet-GATOR2 interaction, show evolved rapidly Dipterans recruit repurpose an independent methyltransferase SAM sensor. Thus, modular allows it co-opt preexisting enzymes expand sensing capabilities, revealing mechanism conferring evolvability on otherwise highly system.

Language: Английский

Citations

1
Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells DOI Creative Commons
Liang-Yu Pang, Steven DeLuca, Haolong Zhu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 1, 2023

Abstract Highly potent animal stem cells either self renew or launch complex differentiation programs, using mechanisms that are only partly understood. Drosophila female germline (GSC) perpetuate without change over evolutionary time and generate cystoblast daughters develop into nurse oocytes. Cystoblasts initiate by generating a transient syncytial state, the cyst, increasing pericentromeric H3K9me3 modification, actions likely to suppress transposable element activity. Relatively open GSC chromatin is further restricted Polycomb repression of testis somatic cell-expressed genes briefly active in early germ cells. Subsequently, Neijre/CBP Myc help upregulate growth reprogram metabolism altering mitochondrial transmembrane transport, gluconeogenesis other processes. In all these respects resembles development totipotent zygote. We propose cell state was shaped need resist transposon activity scales.

Language: Английский

Citations

1
Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells DOI Open Access
Liang-Yu Pang, Steven DeLuca, Haolong Zhu

et al.

Published: Aug. 31, 2023

Highly potent animal stem cells either self renew or launch complex differentiation programs, using mechanisms that are only partly understood. Drosophila female germline (GSC) perpetuate without change over evolutionary time and generate cystoblast daughters develop into nurse oocytes. Cystoblasts initiate by generating a transient syncytial state, the cyst, increasing pericentromeric H3K9me3 modification, actions likely to suppress transposable element activity. Relatively open GSC chromatin is further restricted Polycomb repression of testis somatic cell-expressed genes briefly active in early germ cells. Subsequently, Neijre/CBP Myc help upregulate growth reprogram metabolism altering mitochondrial transmembrane transport, gluconeogenesis other processes. In all these respects resembles development totipotent zygote. We propose cell state was shaped need resist transposon activity scales.

Language: Английский

Citations

1
The Drosophila drop-dead gene is required for eggshell integrity DOI Creative Commons
Tayler D. Sheahan,

Amanpreet Grewal,

Laura E. Korthauer

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(12), P. e0295412 - e0295412

Published: Dec. 5, 2023

The eggshell of the fruit fly Drosophila melanogaster is a useful model for understanding synthesis complex extracellular matrix. synthesized during mid-to-late oogenesis by somatic follicle cells that surround developing oocyte. We previously reported female flies mutant gene drop-dead (drd) are sterile, but underlying cause sterility remained unknown. In this study, we examined role drd in synthesis. show eggs laid females fertilized arrest early embryogenesis, and innermost layer eggshell, vitelline membrane, abnormally permeable to dye these eggs. addition, major membrane proteins fail become crosslinked nonreducible bonds, process normally occurs egg activation following ovulation, as evidenced their solubility detection Western blot contrast, Cp36 protein, which found outer chorion layers becomes normally. To link expression pattern with phenotypes, expressed ovarian beginning mid-oogenesis, and, importantly, all phenotypes could be recapitulated selective knockdown cells. determine whether was required crosslinking itself, performed vitro experiments. membranes control chambers either incubation hyperosmotic medium, activates chambers, or exogenous peroxidase hydrogen peroxide. neither treatment resulted chambers. These results indicate necessary serve substrates peroxidase-mediated cross-linking at end oogenesis.

Language: Английский

Citations

1