Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 20, 2022

Osteoarthritis (OA) is a degenerative illness that greatly impacts the life quality of patients. Currently, therapeutic approaches for OA are very limited in clinical. The extracellular vesicles (EVs) derived from different mesenchymal stem cells displayed prominent effect on OA. But most EVs have resources and risks host rejection, immunological response, etc. Human umbilical cord (hUCMSCs) hold advantages easy availability, minimal immune excellent immunomodulatory effects, although hUCMSCs-EVs seldom been applied Herein, we investigated potential anti-inflammatory effects treatment In our results, promoted polarization M2-type macrophages expression anti-inflammation-related cytokines (IL-10). Notably, supernate M2 induced by inhibited level inflammation-associated factors chondrocytes caused IL-1β. Further, injection articular lumen ameliorated progression exerted chondroprotective based joint model created surgical transection anterior cruciate ligament (ACLT). addition, found five highly enriched miRNAs hUCMSCs-EVs, including has-miR-122-5p, has-miR-148a-3p, has-miR-486-5p, has-miR-let-7a-5p, has-miR-100-5p High-throughput sequencing miRNAs, with targeted genes mainly PI3K-Akt signaling pathway. Furthermore, also detected protein abundance using liquidation chromatography tandem quadrupole mass spectrometry (LC-MS/MS) analysis. Thus, study indicates can alleviate cartilage degradation during progression, mechanically may through delivering key proteins modulating pathway mediated to promote macrophage, exhibiting potent potential. current findings suggest might serve as new reagent therapy

Language: Английский

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Emerging Frontiers in acute kidney injury: The role of extracellular vesicles

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 149 - 170

Published: Feb. 18, 2025

Language: Английский

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Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: May 24, 2022

Exosomes are membrane-enclosed vesicles secreted by cells, containing a variety of biologically active ingredients including proteins, nucleic acids and lipids. In this study, we investigated the therapeutic effects exosomes underlying mechanisms in miniature pig model ischemia/reperfusion-induced acute kidney injury (I/R-AKI). The were extracted from cultured human umbilical cord derived mesenchymal stem cells (hUC-MSCs) infused into I/R AKI. Our results showed that 120 min unilateral ischemia followed reperfusion contralateral nephrectomy resulted renal dysfunction, severe damage, apoptosis necroptosis. Intravenous infusion one dose collected about 4 × 10 8 hUC-MSCs significantly improved function reduced Administration hUC-MSC also expression some pro-inflammatory cytokines/chemokines, decreased infiltration macrophages to injured kidneys suppressed phosphorylation nuclear factor-κB signal transducer activator transcription 3, two transcriptional factors related inflammatory regulation. Moreover, could promote proliferation tubular angiogenesis upregulation Klotho Bone Morphogenetic Protein 7, renoprotective molecules vascular endothelial growth factor A its receptor. Collectively, our suggest injection ameliorate I/R-AKI accelerate cell repair regeneration, may be used as potential biological therapy for Acute patients.

Language: Английский

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Comprehensive insight into endothelial progenitor cell-derived extracellular vesicles as a promising candidate for disease treatment

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: June 7, 2022

Abstract Endothelial progenitor cells (EPCs), which are a type of stem cell, have been found to strong angiogenic and tissue repair capabilities. Extracellular vesicles (EVs) contain many effective components, such as cellular proteins, microRNAs, messenger RNAs, long noncoding can be secreted by different cell types. The functions EVs depend mainly on their parent cells. Many researchers conducted functional studies EPC-derived (EPC-EVs) showed that they exhibit therapeutic effects diseases, cardiovascular disease, acute kidney injury, lung sepsis. In this review article, we comprehensively summarized the biogenesis EPCs potent role EPC-EVs in treatment various diseases. Furthermore, current problems future prospects discussed, further needed compare derived from cells, will contribute accelerated translation these applications clinical setting.

Language: Английский

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New insights into the immune functions of podocytes: the role of complement

Molecular and Cellular Pediatrics, Journal Year: 2023, Volume and Issue: 10(1)

Published: April 15, 2023

Abstract Podocytes are differentiated epithelial cells which play an essential role to ensure a normal function of the glomerular filtration barrier (GFB). In addition their adhesive properties in maintaining integrity barrier, they have other functions, such as synthesis components basement membrane (GBM), production vascular endothelial growth factor (VEGF), release inflammatory proteins, and expression complement components. They also participate crosstalk through multiple signalling pathways, including endothelin-1, VEGF, transforming β (TGFβ), bone morphogenetic protein 7 (BMP-7), latent β-binding 1 (LTBP1), extracellular vesicles. Growing literature suggests that podocytes share many innate adaptive immunity, supporting multifunctional ensuring healthy glomerulus. As consequence, “immune podocyte” dysfunction is thought be involved pathogenesis several diseases, referred “podocytopathies.” Multiple factors like mechanical, oxidative, and/or immunologic stressors can induce cell injury. The system, part both define podocyte damage by mechanisms, reactive oxygen species (ROS) generation, cytokine production, endoplasmic reticulum stress, ultimately affecting cytoskeleton, with subsequent detachment from GBM onset proteinuria. Interestingly, found source target complement-mediated express proteins contribute local activation. At same time, rely on protective mechanisms escape this damage. H (CFH), one main regulators cascade, well membrane-bound CD46 or cofactor (MCP), CD55 decay-accelerating (DAF), CD59 defensin. Further autophagy actin-based endocytosis, homeostasis protection against This review will provide overview immune functions response immune-mediated injury, focusing pathogenic link between

Language: Английский

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Endothelial progenitor cell-derived extracellular vesicles: the world of potential prospects for the treatment of cardiovascular diseases

Cell & Bioscience, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 5, 2024

Cardiovascular diseases (CVDs) have emerged as a predominant threat to human health, surpassing the incidence and mortality rates of neoplastic diseases. Extracellular vesicles (EVs) serve vital mediators in intercellular communication material exchange. Endothelial progenitor cells (EPCs), recognized precursors vascular endothelial (ECs), garnered considerable attention recent years due potential therapeutic value their derived extracellular (EPC-EVs) context CVDs. This comprehensive review systematically explores origins, characteristics, functions EPCs, alongside classification, properties, biogenesis, extraction techniques EVs, with particular emphasis on protective roles Additionally, we delve into essential bioactive components EPC-EVs, including microRNAs, long non-coding RNAs, proteins, analyzing beneficial effects promoting angiogenesis, anti-inflammatory anti-oxidant activities, anti-fibrosis, anti-apoptosis, myocardial regeneration. Furthermore, this comprehensively investigates EPC-EVs across various CVDs, encompassing acute infarction, ischemia-reperfusion injury, atherosclerosis, non-ischemic cardiomyopathies, diabetic cardiovascular disease. Lastly, summarize challenges associated clinical application outline future directions, aiming offer valuable resource for both theoretical insights practical applications managing

Language: Английский

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Extracellular Vesicles Derived from Mesenchymal Stromal Cells Delivered during Hypothermic Oxygenated Machine Perfusion Repair Ischemic/Reperfusion Damage of Kidneys from Extended Criteria Donors

Biology, Journal Year: 2022, Volume and Issue: 11(3), P. 350 - 350

Published: Feb. 22, 2022

The poor availability of kidney for transplantation has led to a search new strategies increase the donor pool. main option is use organs from extended criteria donors. We evaluated effects hypothermic oxygenated perfusion (HOPE) with and without extracellular vesicles (EV) derived mesenchymal stromal cells on ischemic/reperfusion injury marginal kidneys unsuitable transplantation. For normothermic reperfusion (NR), we used artificial blood as substitute red cells. global renal ischemic dam-age score (GRS), analyzed ultrastructure (RU), cytochrome c oxidase (COX) IV-1 (a mitochondrial distress marker), caspase-3 expression, tubular cell proliferation index, hepatocyte growth factor (HGF) vascular endothelial (VEGF) tissue levels, effluent lactate glucose levels. HOPE+EV had lower GRS better RU, higher COX expression HGF VEGF levels than HOPE kidneys. During NR, release effluent, suggesting that gluconeogenesis system in group was pre-served. In conclusion, EV delivery during can be considered organ preservation strategy increasing pool improving transplant outcome.

Language: Английский

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Endothelial progenitor cells in the host defense response

Pharmacology & Therapeutics, Journal Year: 2022, Volume and Issue: 241, P. 108315 - 108315

Published: Nov. 24, 2022

Language: Английский

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GABA alleviates high glucose-induced podocyte injury through dynamically altering the expression of macrophage M1/M2-derived exosomal miR-21a-5p/miR-25-3p

Biochemical and Biophysical Research Communications, Journal Year: 2022, Volume and Issue: 618, P. 38 - 45

Published: June 9, 2022

Language: Английский

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Blood–Brain Barrier Biomarkers before and after Kidney Transplantation

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6628 - 6628

Published: April 1, 2023

Kidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by altered levels circulating BBB-specific biomarkers. This study compares neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament light chain (NfL), and plasma extracellular vesicles (EVs) in kidney-failure patients before KT at a two-year follow up. Using ELISA, NSE, BDNF, NfL were measured 74 living-donor patients. Plasma EVs isolated with ultracentrifugation, characterized for concentration/size surface protein expression using flow cytometry from subset 25 Lower NSE levels, higher BDNF observed follow-up compared to baseline (p < 0.05). Male had significantly those females. BBB biomarkers correlated lipid profile glucose, vitamin D, inflammation markers after KT. surrogate marker changes microcirculation early vascular aging phenotype calcification and/or fibrosis only BDNF. CD31+ microparticles endothelial cells expressing inflammatory such as CD40 integrins reduced may, thus, CKD

Language: Английский

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