Uncovering Astrocyte Morphological Dynamics Using Optical Diffraction Tomography and Shape‐Based Trajectory Inference DOI
Pooja Anantha, Piyush Raj, Emanuela Saracino

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Astrocytes, integral components of the central nervous system, are increasingly recognized for their multifaceted roles beyond support cells. Despite acknowledged importance, understanding intricacies astrocyte morphological dynamics remains limited. Our study marks first exploration astrocytes using optical diffraction tomography (ODT), establishing a label-free, quantitative method to observe changes in over 7-day in-vitro period. ODT offers insights into cell volume, dry mass, and area through real-time measurements-capabilities that challenging achieve with conventional imaging techniques. Through comprehensive analysis 3D refractive index maps shape characterization techniques, we capture developmental trajectory dynamic transformations astrocytes. Specifically, our observations reveal increased transition larger, flattened shapes, alterations volume density, indicating shifts cellular composition. By employing unsupervised clustering pseudotime analysis, introduce novel inference neural cells, tracking evolution from elongated evenly spread shapes. This use based solely on morphology types, laying foundation future studies examine interactions across diverse substrates.

Language: Английский

Advancing cell therapy for neurodegenerative diseases DOI Creative Commons
Sally Temple

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(5), P. 512 - 529

Published: April 20, 2023

Language: Английский

Citations

90
Precision drug delivery to the central nervous system using engineered nanoparticles DOI
Jingjing Gao, Ziting Xia,

Swetharajan Gunasekar

et al.

Nature Reviews Materials, Journal Year: 2024, Volume and Issue: 9(8), P. 567 - 588

Published: June 25, 2024

Language: Английский

Citations

27
Endocannabinoid signaling in astrocytes DOI Creative Commons
Abel Eraso‐Pichot, Sandrine Pouvreau, Alexandre Oliveira-Pinto

et al.

Glia, Journal Year: 2022, Volume and Issue: 71(1), P. 44 - 59

Published: July 13, 2022

Abstract The study of the astrocytic contribution to brain functions has been growing in popularity neuroscience field. In last years, and especially since demonstration involvement astrocytes synaptic functions, astrocyte field revealed multiple these cells that seemed inconceivable not long ago. parallel, cannabinoid investigation also identified different ways by which cannabinoids are able interact with cells, modify their alter communication neurons impact behavior. this review, we will describe expression endocannabinoid system members astrocytes. Moreover, relate latest findings regarding modulation some most relevant astroglial namely calcium (Ca 2+ ) dynamics, gliotransmission, metabolism, inflammation.

Language: Английский

Citations

54
Therapeutic non-invasive brain treatments in Alzheimer’s disease: recent advances and challenges DOI Creative Commons

Chongyun Wu,

Luoman Yang,

Shu Feng

et al.

Inflammation and Regeneration, Journal Year: 2022, Volume and Issue: 42(1)

Published: Oct. 3, 2022

Abstract Alzheimer’s disease (AD) is one of the major neurodegenerative diseases and most common form dementia. Characterized by loss learning, memory, problem-solving, language, other thinking abilities, AD exerts a detrimental effect on both patients’ families’ quality life. Although there have been significant advances in understanding mechanism underlying pathogenesis progression AD, no cure for AD. The failure numerous molecular targeted pharmacologic clinical trials leads to an emerging research shift toward non-invasive therapies, especially multiple treatments. In this paper, we reviewed widely studied including photobiomodulation (PBM), transcranial magnetic stimulation (TMS), direct current (tDCS), exercise therapy. Firstly, pathological changes challenges studies. We then introduced these therapies discussed factors that may affect effects therapies. Additionally, review possible mechanisms effects. Finally, summarized treatments future studies applications. concluded it would be critical understand exact find optimal treatment parameters improve translational value Moreover, combined use also promising direction sheds light or prevention

Language: Английский

Citations

48
Pathogenesis of α-Synuclein in Parkinson’s Disease: From a Neuron-Glia Crosstalk Perspective DOI Open Access
Shuanglong Yi, Linfang Wang, Honglei Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 14753 - 14753

Published: Nov. 25, 2022

Parkinson's disease (PD) is a progressive neurodegenerative disorder. The classical behavioral defects of PD patients involve motor symptoms such as bradykinesia, tremor, and rigidity, well non-motor anosmia, depression, cognitive impairment. Pathologically, the loss dopaminergic (DA) neurons in substantia nigra (SN) accumulation α-synuclein (α-syn)-composed Lewy bodies (LBs) neurites (LNs) are key hallmarks. Glia more than mere bystanders that simply support neurons, they actively contribute to almost every aspect neuronal development function; glial dysregulation has been implicated series diseases including PD. Importantly, amounting evidence added activation neuroinflammation new features onset progression. Thus, gaining better understanding glia, especially neuron-glia crosstalk, will not only provide insight into brain physiology events but also advance our knowledge pathologies. This review addresses current α-syn pathogenesis PD, with focus on crosstalk. Particularly, transmission between α-syn-induced activation, feedbacks DA neuron degeneration thoroughly discussed. In addition, aggregation, iron deposition, regulating ferroptosis covered. Lastly, we summarize preclinical clinical therapies, targeting treatments.

Language: Английский

Citations

44
Astroglial Cells: Emerging Therapeutic Targets in the Management of Traumatic Brain Injury DOI Creative Commons
Wojciech Czyżewski, Marek Mazurek, Leon Sakwa

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(2), P. 148 - 148

Published: Jan. 12, 2024

Traumatic Brain Injury (TBI) represents a significant health concern, necessitating advanced therapeutic interventions. This detailed review explores the critical roles of astrocytes, key cellular constituents central nervous system (CNS), in both pathophysiology and possible rehabilitation TBI. Following injury, astrocytes exhibit reactive transformations, differentiating into pro-inflammatory (A1) neuroprotective (A2) phenotypes. paper elucidates interactions with neurons, their role neuroinflammation, potential for exploitation. Emphasized strategies encompass utilization endocannabinoid calcium signaling pathways, hormone-based treatments like 17β-estradiol, biological therapies employing anti-HBGB1 monoclonal antibodies, gene therapy targeting Connexin 43, innovative technique astrocyte transplantation as means to repair damaged neural tissues.

Language: Английский

Citations

9
Mesenchymal Stem Cell Therapy in Traumatic Spinal Cord Injury: A Systematic Review DOI Open Access
Rodrigo Montoto-Meijide, Rosa Meijide-Faílde, Silvia Díaz‐Prado

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11719 - 11719

Published: July 20, 2023

Recovery from a traumatic spinal cord injury (TSCI) is challenging due to the limited regenerative capacity of central nervous system restore cells, myelin, and neural connections. Cell therapy, particularly with mesenchymal stem cells (MSCs), holds significant promise for TSCI treatment. This systematic review aims analyze efficacy, safety, therapeutic potential MSC-based cell therapies in TSCI. A comprehensive search PUBMED COCHRANE databases until February 2023 was conducted, combining terms such as “spinal injury,” “stem cells,” therapy,” “mesenchymal “traumatic injury”. Among 53 studies initially identified, 22 (21 clinical trials 1 case series) were included. Findings these consistently demonstrate improvements AIS (ASIA Impairment Scale) grades, sensory scores, and, lesser extent, motor scores. Meta-analyses further support positive outcomes. have shown short- medium-term indicated by absence adverse events within studied timeframe. However, caution required when drawing generalized recommendations scientific evidence available. Further research needed elucidate long-term safety implications advancements. Although progress has been made, therapies, additional exploring other future gene neurostimulation techniques, tissue engineering approaches are essential understanding evolving treatment landscape.

Language: Английский

Citations

20
Astrocytes: Dissecting Their Diverse Roles in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia DOI Creative Commons
Chiara F. Valori,

Claudia Sulmona,

Liliana Brambilla

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(11), P. 1450 - 1450

Published: May 23, 2023

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are fatal neurodegenerative disorders often co-occurring in the same patient, a feature that suggests common origin of two diseases. Consistently, pathological inclusions proteins as well mutations genes can be identified both ALS/FTD. Although many studies have described several disrupted pathways within neurons, glial cells also regarded crucial pathogenetic contributors Here, we focus our attention on astrocytes, heterogenous population perform functions for optimal central nervous system homeostasis. Firstly, discuss how post-mortem material from ALS/FTD patients supports astrocyte dysfunction around three pillars: neuroinflammation, abnormal protein aggregation, atrophy/degeneration. Furthermore, summarize current attempts at monitoring living using either novel imaging strategies or soluble biomarkers. We then address pathology is recapitulated animal cellular models used these to understand molecular mechanisms driving platforms pre-clinical testing therapeutics. Finally, present clinical trials ALS/FTD, restricting discussion treatments modulate functions, directly indirectly.

Language: Английский

Citations

19
Advances in Delivering Oxidative Modulators for Disease Therapy DOI Creative Commons
Wei Yang, Hua Yue, Guihong Lu

et al.

Research, Journal Year: 2022, Volume and Issue: 2022

Published: Jan. 1, 2022

Oxidation modulators regarding antioxidants and reactive oxygen species (ROS) inducers have been used for the treatment of many diseases. However, a systematic review that refers to delivery system divergent modulation oxidative level within biomedical scope is lacking. To provide comprehensive summarization analysis, we pilot designs delivering main applications inflammatory tumor therapy. On one hand, based can be employed downregulate ROS levels at sites treat diseases (e.g., skin repair, bone-related diseases, organ dysfunction, neurodegenerative diseases). other upregulate site kill cells disrupt endogenous balance induce lethal ROS). Besides current systems application cases, prospects future research are also provided identify intelligent strategies inspire new concepts modulators.

Language: Английский

Citations

26
Neurodegeneration and inflammation crosstalk: Therapeutic targets and perspectives DOI Creative Commons
Wael Mohamed, Jaya Kumar, Badrah S. Alghamdi

et al.

IBRO Neuroscience Reports, Journal Year: 2022, Volume and Issue: 14, P. 95 - 110

Published: Dec. 16, 2022

Glia, which was formerly considered to exist just connect neurons, now plays a key function in wide range of physiological events, including formation memory, learning, neuroplasticity, synaptic plasticity, energy consumption, and homeostasis ions. Glial cells regulate the brain's immune responses confers nutritional structural aid making them an important player broad neurological disorders. Alzheimer's, ALS, Parkinson's, frontotemporal dementia (FTD), epilepsy are few neurodegenerative diseases that have been linked microglia astroglia cells, particular. Synapse growth is aided by glial cell activity, this activity has effect on neuronal signalling. Each malfunction diverse distinct, we will discuss its significance progression illness, as well potential for future treatment.

Language: Английский

Citations

25