Drosophila as a Model to Understand Second Heart Field Development DOI Creative Commons

Cayleen Bileckyj,

Brenna Blotz,

Richard M. Cripps

et al.

Journal of Cardiovascular Development and Disease, Journal Year: 2023, Volume and Issue: 10(12), P. 494 - 494

Published: Dec. 12, 2023

The genetic model system Drosophila has contributed fundamentally to our understanding of mammalian heart specification, development, and congenital disease. relatively simple is a linear muscular tube that specified develops in the embryo persists throughout life animal. It functions at all stages circulate hemolymph within open circulatory body. During metamorphosis, cardiac remodeled, new layer muscle fibers spreads over ventral surface form longitudinal muscles. formation these depends critically upon genes known be necessary for second field (SHF) formation. Here, we review prior contributions development disease, discuss importance SHF then how adult muscles can serve as novel

Language: Английский

Preclinical Models of Cardiac Disease: A Comprehensive Overview for Clinical Scientists DOI Creative Commons

Elisa C. H. van Doorn,

Jorik H. Amesz, Amir H. Sadeghi

et al.

Cardiovascular Engineering and Technology, Journal Year: 2024, Volume and Issue: 15(2), P. 232 - 249

Published: Jan. 16, 2024

Abstract For recent decades, cardiac diseases have been the leading cause of death and morbidity worldwide. Despite significant achievements in their management, profound understanding disease progression is limited. The lack biologically relevant robust preclinical models that truly grasp molecular underpinnings its pathophysiology attributes to this stagnation, as well insufficiency platforms effectively explore novel therapeutic avenues. area fundamental translational research has therefore gained wide interest scientists clinical field, while landscape rapidly evolved towards an elaborate array modalities, characterized by diverse distinctive traits. As a consequence, current literature lacks intelligible complete overview aimed at focuses on selecting optimal platform for questions. In review, we present vitro, ex vivo, vivo silico model health disease, delineating main benefits drawbacks, innovative prospects, foremost fields application scope incentives.

Language: Английский

Citations

12
EFFICIENT RIBOSOMAL RNA DEPLETION FROM DROSOPHILA TOTAL RNA FOR NEXT-GENERATION SEQUENCING APPLICATIONS DOI Creative Commons

Omkar Koppaka,

Shweta Tandon, Ankita Chodankar

et al.

Wellcome Open Research, Journal Year: 2025, Volume and Issue: 10, P. 112 - 112

Published: May 13, 2025

We developed a cost-effective enzyme-based rRNA-depletion method tailored for Drosophila melanogaster, addressing the limitations of existing commercial kits and lack peer-reviewed alternatives. Our employs single-stranded DNA probes complementary to Drosophila rRNA, forming DNA-RNA hybrids. These hybrids are then degraded using RNase H enzyme, effectively removing rRNA enriching all non-ribosomal RNAs, including mRNA, lncRNA small RNA. When compared removal kit, our approach demonstrated superior efficiency mapping percentage, confirming its effectiveness. Additionally, successfully enriched non-coding transcriptome, making it valuable tool studying ncRNA in Drosophila. The probe sequences protocol made freely available, offering reliable alternative experiments.

Language: Английский

Citations

1
The hypertrophic cardiomyopathy-associated A331P actin variant enhances basal contractile activity and elicits resting muscle dysfunction DOI Creative Commons
Matthew Doran, Michael J. Rynkiewicz,

Evan A. Despond

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(2), P. 111816 - 111816

Published: Jan. 18, 2025

Previous studies aimed at defining the mechanistic basis of hypertrophic cardiomyopathy caused by A331P cardiac actin have reported conflicting results. The mutation is located along an surface strand, proximal to residues that interact with tropomyosin. These F-actin-tropomyosin associations are vital for proper contractile inhibition. To help resolve disease pathogenesis, we implemented a multidisciplinary approach. Transgenic Drosophila, expressing actin, displayed skeletal muscle hypercontraction and elevated basal myocardial activity. thin filaments, reconstituted using recombinant human exhibited higher in vitro myosin-based sliding speeds, exclusively low Ca2+ concentrations. Cryo-EM-based reconstructions revealed no detectable A331P-related structural perturbations F-actin. In silico, however, P331-containing strand was less mobile established diminished van der Waal's attractive forces tropomyosin, which correlated greater variability inhibitory tropomyosin positioning. Such mutation-induced effects potentially elevate resting activity among our models may stimulate pathology patients.

Language: Английский

Citations

0
Investigation regarding the physiological effects of cobalt on physiological functions in Drosophila, crayfish, and crab: Behavioral, cardiac, neural, and synaptic properties DOI

Joseph J. Gard,

Kaitlyn E. Brock,

Elizabeth R. Elliott

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2025, Volume and Issue: 292, P. 110165 - 110165

Published: Feb. 26, 2025

Language: Английский

Citations

0
EFFICIENT RIBOSOMAL RNA DEPLETION FROM DROSOPHILA TOTAL RNA FOR NEXT-GENERATION SEQUENCING APPLICATIONS DOI Creative Commons

Omkar Koppaka,

Shweta Tandon, Ankita Chodankar

et al.

Wellcome Open Research, Journal Year: 2025, Volume and Issue: 10, P. 112 - 112

Published: Feb. 28, 2025

We developed a cost-effective enzyme-based rRNA-depletion method tailored for Drosophila melanogaster, addressing the limitations of existing commercial kits and lack peer-reviewed alternatives. Our employs single-stranded DNA probes complementary to Drosophila rRNA, forming DNA-RNA hybrids. These hybrids are then degraded using RNase H enzyme, effectively removing rRNA enriching all non-ribosomal RNAs, including mRNA, lncRNA small RNA. When compared removal kit, our approach demonstrated superior efficiency mapping percentage, confirming its effectiveness. Additionally, successfully enriched non-coding transcriptome, making it valuable tool studying ncRNA in Drosophila. The probe sequences protocol made freely available, offering reliable alternative experiments.

Language: Английский

Citations

0
Endurance Exercise Ameliorates Aging-Related Bradyarrhythmia in Drosophila Resulting from miR-283 Knockdown in LNvs DOI Open Access
Qiufang Li, Ping Xu,

Zhengwen Yu

et al.

Published: April 11, 2025

MicroRNAs (miRNAs) are crucial in regulating cardiac aging and related diseases, yet few functional miRNAs have been identified. Prior studies showed miR-216a upregulation heart failure patients, but its impact on hearts is unknown. Our study revealed systemic miR-283 overexpression or knockdown caused age-related bradycardia, mimicking human bradyarrhythmia. Importantly, we found that of ventral-lateral neurons (LN v s), rather than the heart, led to occurrence bradyarrhythmia, which was mainly by expression whole brain heart. The gene clockwork orange ( cwo ) may mediate miR-283’s effect rhythm. Additionally, investigate miRNA regulatory mechanism underlying exercise-induced delay aging, conducted a three-week endurance exercise program flies LN s. We significantly downregulated accumulation myocardium s, improved structure myocardial fibers, effectively reduced findings provides new perspective distal neuromodulation intervention aging.

Language: Английский

Citations

0
Endurance Exercise Ameliorates Aging-Related Bradyarrhythmia in Drosophila Resulting from miR-283 Knockdown in LNvs DOI Open Access
Qiufang Li, Ping Xu,

Zhengwen Yu

et al.

Published: April 11, 2025

MicroRNAs (miRNAs) are crucial in regulating cardiac aging and related diseases, yet few functional miRNAs have been identified. Prior studies showed miR-216a upregulation heart failure patients, but its impact on hearts is unknown. Our study revealed systemic miR-283 overexpression or knockdown caused age-related bradycardia, mimicking human bradyarrhythmia. Importantly, we found that of ventral-lateral neurons (LN v s), rather than the heart, led to occurrence bradyarrhythmia, which was mainly by expression whole brain heart. The gene clockwork orange ( cwo ) may mediate miR-283’s effect rhythm. Additionally, investigate miRNA regulatory mechanism underlying exercise-induced delay aging, conducted a three-week endurance exercise program flies LN s. We significantly downregulated accumulation myocardium s, improved structure myocardial fibers, effectively reduced findings provides new perspective distal neuromodulation intervention aging.

Language: Английский

Citations

0
When the end modifies its means: the origins of novelty and the evolution of innovation DOI Open Access
Armin P. Moczek

Biological Journal of the Linnean Society, Journal Year: 2022, Volume and Issue: 139(4), P. 433 - 440

Published: July 6, 2022

Abstract The origin of novel complex traits constitutes a central yet largely unresolved challenge in evolutionary biology. Intriguingly, many the most promising breakthroughs understanding genesis novelty recent years have occurred not biology itself, but through comparative study development and, more recently, interface developmental and ecology. Here, I discuss how these insights are changing our what matters novel, ontogeny evolution. Specifically, my essay has two major objectives. First, nature systems biases production phenotypic variation face or stressful environments toward functional, integrated possibly, adaptive variants. This, turn, allows phenotypes to precede (rather than follow) changes genotype processes that product past evolution shape change occur. Second, explore this itself evolved over time, increasing repertoire ontogenies pursue wider range objectives across an expanding conditions, thereby creating increasingly extensive affordance landscape Developmental their can thus be viewed as dynamic modify own means phylogeny. dynamics necessitates strict reductionist approach currently dominates fields

Language: Английский

Citations

16
Deregulations of miR‐1 and its target Multiplexin promote dilated cardiomyopathy associated with myotonic dystrophy type 1 DOI Creative Commons

Anissa Souidi,

Masayuki Nakamori, Monika Zmojdzian

et al.

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(4)

Published: Feb. 28, 2023

Abstract Myotonic dystrophy type 1 (DM1) is the most common muscular in adults. It caused by excessive expansion of noncoding CTG repeats, which when transcribed affects functions RNA‐binding factors with adverse effects on alternative splicing, processing, and stability a large set cardiac transcripts. Among these effects, inefficient processing down‐regulation muscle‐ heart‐specific miRNA, miR‐1 , have been reported DM1 patients, but impact reduced pathogenesis has unknown. Here, we use Drosophila models to explore role dysfunction DM1. We show that heart leads dilated cardiomyopathy (DCM), DM1‐associated phenotype. combined silico screening for targets transcriptional profiling cells identify target genes potential roles DCM. Multiplexin (Mp) as new involved Mp encodes collagen protein tube formation . its human ortholog Col15A1 are both highly enriched DCM‐developing flies samples from patients DCM, respectively. When overexpressed heart, induces whereas attenuation rescues DCM phenotype aged flies. Reduced levels consecutive up‐regulation Mp/Col15A1 might be critical

Language: Английский

Citations

9
Silibinin alleviates intestinal inflammation via inhibiting JNK signaling in Drosophila DOI Creative Commons

La Yan,

Juanyu Zhou,

Lu Yuan

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 14, 2023

Inflammatory bowel diseases (IBDs) are characterized by chronic relapsing intestinal inflammation that causes digestive system dysfunction. For years, researchers have been working to find more effective and safer therapeutic strategies treat these diseases. Silibinin (SIL), a flavonoid compound extracted from the seeds of milk thistle plants, possesses multiple biological activities is traditionally applied liver SIL also widely used in treatment variety inflammatory attributed its excellent antioxidant anti-inflammatory effects. However, efficacy against IBDs mechanisms remain unclear. In this study, using Drosophila melanogaster as model organism, we found can effectively relieve caused dextran sulfate sodium (DSS). Our results suggested supplementation inhibit overproliferation stem cells (ISCs) induced DSS, protect barrier function, acid-base balance, excretion reduce reactive oxygen species (ROS) levels stress, extend lifespan Drosophila. Furthermore, our study demonstrated ameliorates via modulating c-Jun N-terminal kinase (JNK) signaling pathway research aims provide new insight into IBDs.

Language: Английский

Citations

9