Journal of Cardiovascular Development and Disease,
Journal Year:
2023,
Volume and Issue:
10(12), P. 494 - 494
Published: Dec. 12, 2023
The
genetic
model
system
Drosophila
has
contributed
fundamentally
to
our
understanding
of
mammalian
heart
specification,
development,
and
congenital
disease.
relatively
simple
is
a
linear
muscular
tube
that
specified
develops
in
the
embryo
persists
throughout
life
animal.
It
functions
at
all
stages
circulate
hemolymph
within
open
circulatory
body.
During
metamorphosis,
cardiac
remodeled,
new
layer
muscle
fibers
spreads
over
ventral
surface
form
longitudinal
muscles.
formation
these
depends
critically
upon
genes
known
be
necessary
for
second
field
(SHF)
formation.
Here,
we
review
prior
contributions
development
disease,
discuss
importance
SHF
then
how
adult
muscles
can
serve
as
novel
Cardiovascular Engineering and Technology,
Journal Year:
2024,
Volume and Issue:
15(2), P. 232 - 249
Published: Jan. 16, 2024
Abstract
For
recent
decades,
cardiac
diseases
have
been
the
leading
cause
of
death
and
morbidity
worldwide.
Despite
significant
achievements
in
their
management,
profound
understanding
disease
progression
is
limited.
The
lack
biologically
relevant
robust
preclinical
models
that
truly
grasp
molecular
underpinnings
its
pathophysiology
attributes
to
this
stagnation,
as
well
insufficiency
platforms
effectively
explore
novel
therapeutic
avenues.
area
fundamental
translational
research
has
therefore
gained
wide
interest
scientists
clinical
field,
while
landscape
rapidly
evolved
towards
an
elaborate
array
modalities,
characterized
by
diverse
distinctive
traits.
As
a
consequence,
current
literature
lacks
intelligible
complete
overview
aimed
at
focuses
on
selecting
optimal
platform
for
questions.
In
review,
we
present
vitro,
ex
vivo,
vivo
silico
model
health
disease,
delineating
main
benefits
drawbacks,
innovative
prospects,
foremost
fields
application
scope
incentives.
Wellcome Open Research,
Journal Year:
2025,
Volume and Issue:
10, P. 112 - 112
Published: May 13, 2025
We
developed
a
cost-effective
enzyme-based
rRNA-depletion
method
tailored
for
Drosophila
melanogaster,
addressing
the
limitations
of
existing
commercial
kits
and
lack
peer-reviewed
alternatives.
Our
employs
single-stranded
DNA
probes
complementary
to
Drosophila
rRNA,
forming
DNA-RNA
hybrids.
These
hybrids
are
then
degraded
using
RNase
H
enzyme,
effectively
removing
rRNA
enriching
all
non-ribosomal
RNAs,
including
mRNA,
lncRNA
small
RNA.
When
compared
removal
kit,
our
approach
demonstrated
superior
efficiency
mapping
percentage,
confirming
its
effectiveness.
Additionally,
successfully
enriched
non-coding
transcriptome,
making
it
valuable
tool
studying
ncRNA
in
Drosophila.
The
probe
sequences
protocol
made
freely
available,
offering
reliable
alternative
experiments.
iScience,
Journal Year:
2025,
Volume and Issue:
28(2), P. 111816 - 111816
Published: Jan. 18, 2025
Previous
studies
aimed
at
defining
the
mechanistic
basis
of
hypertrophic
cardiomyopathy
caused
by
A331P
cardiac
actin
have
reported
conflicting
results.
The
mutation
is
located
along
an
surface
strand,
proximal
to
residues
that
interact
with
tropomyosin.
These
F-actin-tropomyosin
associations
are
vital
for
proper
contractile
inhibition.
To
help
resolve
disease
pathogenesis,
we
implemented
a
multidisciplinary
approach.
Transgenic
Drosophila,
expressing
actin,
displayed
skeletal
muscle
hypercontraction
and
elevated
basal
myocardial
activity.
thin
filaments,
reconstituted
using
recombinant
human
exhibited
higher
in
vitro
myosin-based
sliding
speeds,
exclusively
low
Ca2+
concentrations.
Cryo-EM-based
reconstructions
revealed
no
detectable
A331P-related
structural
perturbations
F-actin.
In
silico,
however,
P331-containing
strand
was
less
mobile
established
diminished
van
der
Waal's
attractive
forces
tropomyosin,
which
correlated
greater
variability
inhibitory
tropomyosin
positioning.
Such
mutation-induced
effects
potentially
elevate
resting
activity
among
our
models
may
stimulate
pathology
patients.
Wellcome Open Research,
Journal Year:
2025,
Volume and Issue:
10, P. 112 - 112
Published: Feb. 28, 2025
We
developed
a
cost-effective
enzyme-based
rRNA-depletion
method
tailored
for
Drosophila
melanogaster,
addressing
the
limitations
of
existing
commercial
kits
and
lack
peer-reviewed
alternatives.
Our
employs
single-stranded
DNA
probes
complementary
to
Drosophila
rRNA,
forming
DNA-RNA
hybrids.
These
hybrids
are
then
degraded
using
RNase
H
enzyme,
effectively
removing
rRNA
enriching
all
non-ribosomal
RNAs,
including
mRNA,
lncRNA
small
RNA.
When
compared
removal
kit,
our
approach
demonstrated
superior
efficiency
mapping
percentage,
confirming
its
effectiveness.
Additionally,
successfully
enriched
non-coding
transcriptome,
making
it
valuable
tool
studying
ncRNA
in
Drosophila.
The
probe
sequences
protocol
made
freely
available,
offering
reliable
alternative
experiments.
MicroRNAs
(miRNAs)
are
crucial
in
regulating
cardiac
aging
and
related
diseases,
yet
few
functional
miRNAs
have
been
identified.
Prior
studies
showed
miR-216a
upregulation
heart
failure
patients,
but
its
impact
on
hearts
is
unknown.
Our
study
revealed
systemic
miR-283
overexpression
or
knockdown
caused
age-related
bradycardia,
mimicking
human
bradyarrhythmia.
Importantly,
we
found
that
of
ventral-lateral
neurons
(LN
v
s),
rather
than
the
heart,
led
to
occurrence
bradyarrhythmia,
which
was
mainly
by
expression
whole
brain
heart.
The
gene
clockwork
orange
(
cwo
)
may
mediate
miR-283’s
effect
rhythm.
Additionally,
investigate
miRNA
regulatory
mechanism
underlying
exercise-induced
delay
aging,
conducted
a
three-week
endurance
exercise
program
flies
LN
s.
We
significantly
downregulated
accumulation
myocardium
s,
improved
structure
myocardial
fibers,
effectively
reduced
findings
provides
new
perspective
distal
neuromodulation
intervention
aging.
MicroRNAs
(miRNAs)
are
crucial
in
regulating
cardiac
aging
and
related
diseases,
yet
few
functional
miRNAs
have
been
identified.
Prior
studies
showed
miR-216a
upregulation
heart
failure
patients,
but
its
impact
on
hearts
is
unknown.
Our
study
revealed
systemic
miR-283
overexpression
or
knockdown
caused
age-related
bradycardia,
mimicking
human
bradyarrhythmia.
Importantly,
we
found
that
of
ventral-lateral
neurons
(LN
v
s),
rather
than
the
heart,
led
to
occurrence
bradyarrhythmia,
which
was
mainly
by
expression
whole
brain
heart.
The
gene
clockwork
orange
(
cwo
)
may
mediate
miR-283’s
effect
rhythm.
Additionally,
investigate
miRNA
regulatory
mechanism
underlying
exercise-induced
delay
aging,
conducted
a
three-week
endurance
exercise
program
flies
LN
s.
We
significantly
downregulated
accumulation
myocardium
s,
improved
structure
myocardial
fibers,
effectively
reduced
findings
provides
new
perspective
distal
neuromodulation
intervention
aging.
Biological Journal of the Linnean Society,
Journal Year:
2022,
Volume and Issue:
139(4), P. 433 - 440
Published: July 6, 2022
Abstract
The
origin
of
novel
complex
traits
constitutes
a
central
yet
largely
unresolved
challenge
in
evolutionary
biology.
Intriguingly,
many
the
most
promising
breakthroughs
understanding
genesis
novelty
recent
years
have
occurred
not
biology
itself,
but
through
comparative
study
development
and,
more
recently,
interface
developmental
and
ecology.
Here,
I
discuss
how
these
insights
are
changing
our
what
matters
novel,
ontogeny
evolution.
Specifically,
my
essay
has
two
major
objectives.
First,
nature
systems
biases
production
phenotypic
variation
face
or
stressful
environments
toward
functional,
integrated
possibly,
adaptive
variants.
This,
turn,
allows
phenotypes
to
precede
(rather
than
follow)
changes
genotype
processes
that
product
past
evolution
shape
change
occur.
Second,
explore
this
itself
evolved
over
time,
increasing
repertoire
ontogenies
pursue
wider
range
objectives
across
an
expanding
conditions,
thereby
creating
increasingly
extensive
affordance
landscape
Developmental
their
can
thus
be
viewed
as
dynamic
modify
own
means
phylogeny.
dynamics
necessitates
strict
reductionist
approach
currently
dominates
fields
EMBO Reports,
Journal Year:
2023,
Volume and Issue:
24(4)
Published: Feb. 28, 2023
Abstract
Myotonic
dystrophy
type
1
(DM1)
is
the
most
common
muscular
in
adults.
It
caused
by
excessive
expansion
of
noncoding
CTG
repeats,
which
when
transcribed
affects
functions
RNA‐binding
factors
with
adverse
effects
on
alternative
splicing,
processing,
and
stability
a
large
set
cardiac
transcripts.
Among
these
effects,
inefficient
processing
down‐regulation
muscle‐
heart‐specific
miRNA,
miR‐1
,
have
been
reported
DM1
patients,
but
impact
reduced
pathogenesis
has
unknown.
Here,
we
use
Drosophila
models
to
explore
role
dysfunction
DM1.
We
show
that
heart
leads
dilated
cardiomyopathy
(DCM),
DM1‐associated
phenotype.
combined
silico
screening
for
targets
transcriptional
profiling
cells
identify
target
genes
potential
roles
DCM.
Multiplexin
(Mp)
as
new
involved
Mp
encodes
collagen
protein
tube
formation
.
its
human
ortholog
Col15A1
are
both
highly
enriched
DCM‐developing
flies
samples
from
patients
DCM,
respectively.
When
overexpressed
heart,
induces
whereas
attenuation
rescues
DCM
phenotype
aged
flies.
Reduced
levels
consecutive
up‐regulation
Mp/Col15A1
might
be
critical
Frontiers in Pharmacology,
Journal Year:
2023,
Volume and Issue:
14
Published: Sept. 14, 2023
Inflammatory
bowel
diseases
(IBDs)
are
characterized
by
chronic
relapsing
intestinal
inflammation
that
causes
digestive
system
dysfunction.
For
years,
researchers
have
been
working
to
find
more
effective
and
safer
therapeutic
strategies
treat
these
diseases.
Silibinin
(SIL),
a
flavonoid
compound
extracted
from
the
seeds
of
milk
thistle
plants,
possesses
multiple
biological
activities
is
traditionally
applied
liver
SIL
also
widely
used
in
treatment
variety
inflammatory
attributed
its
excellent
antioxidant
anti-inflammatory
effects.
However,
efficacy
against
IBDs
mechanisms
remain
unclear.
In
this
study,
using
Drosophila
melanogaster
as
model
organism,
we
found
can
effectively
relieve
caused
dextran
sulfate
sodium
(DSS).
Our
results
suggested
supplementation
inhibit
overproliferation
stem
cells
(ISCs)
induced
DSS,
protect
barrier
function,
acid-base
balance,
excretion
reduce
reactive
oxygen
species
(ROS)
levels
stress,
extend
lifespan
Drosophila.
Furthermore,
our
study
demonstrated
ameliorates
via
modulating
c-Jun
N-terminal
kinase
(JNK)
signaling
pathway
research
aims
provide
new
insight
into
IBDs.