Transcriptional regulation of fleshy fruit texture

Journal of Integrative Plant Biology, Journal Year: 2022, Volume and Issue: 64(9), P. 1649 - 1672

Published: June 22, 2022

Abstract Fleshy fruit texture is a critically important quality characteristic of ripe fruit. Softening an irreversible process which operates in most fleshy fruits during ripening which, together with changes color and taste, contributes to improvements mouthfeel general attractiveness. results mainly from the expression genes encoding enzymes responsible for cell wall modifications but starch degradation high levels flavonoids can also contribute change. Some undergo lignification development post‐harvest, negatively affects eating quality. Excessive softening lead physical damage infection, particularly transport storage causes severe supply chain losses. Many transcription factors (TFs) that regulate by controlling involved metabolism have been characterized. TFs directly targets, while others act as part broader regulatory program governing several aspects process. In this review, we focus on advances our understanding transcriptional mechanisms textural change development, post‐harvest. Potential targets breeding future research directions control improvement are discussed.

Language: Английский

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CRISPR/Cas9-mediated SNAC9 mutants reveal the positive regulation of tomato ripening by SNAC9 and the mechanism of carotenoid metabolism regulation

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(4)

Published: Feb. 10, 2023

NAC transcriptional regulators are crucial for tomato ripening. Virus-induced gene silencing (VIGS) of

Language: Английский

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Proteome and metabolome of Caryocar brasiliense camb. fruit and their interaction during development

Food Research International, Journal Year: 2024, Volume and Issue: 191, P. 114687 - 114687

Published: June 28, 2024

Language: Английский

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Functional redundancy of transcription factors SlNOR and SlNOR-like1 is required for pollen development in tomato

Horticulture Research, Journal Year: 2025, Volume and Issue: 12(4)

Published: Jan. 6, 2025

In tomato, SlNOR and SlNOR-like1, members of the NAC family transcription factors (TFs), are known to play critical roles in regulating fruit ripening highly expressed floral organs. However, their role flower development remains unclear. this study, we generated functionally characterized a double knockout mutant, nor/nor-like1. Our findings reveal that pollen abortion nor/nor-like1 impedes ovarian enlargement, resulting formation failure. Histological analyses demonstrate wall collapse occurs during mature stage leads abnormal component deposition at microspore stage, male sterility mutant lines. Kyoto Encyclopedia Genes Genomes enrichment pathway further suggest loss SlNOR-like1 function affects several metabolic pathways related development, including 'ABC transporters', 'lipid metabolism', 'phenylpropanoid biosynthesis', 'hormone signal transduction', 'starch sucrose 'cutin, suberine, wax biosynthesis'. Furthermore, our results could directly bind promoters key genes associated with activate expression, ATP-binding cassette transporters G (SlABCG8/9/23), ECERIFERUM (SlCER1), glycine-rich protein (SlGRP92). These may redundant biosynthesis transport sporopollenin precursors, cuticular biosynthesis, exine formation. summary, study highlights previously uncharacterized tomato fertility.

Language: Английский

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Genome-Wide Analysis of the PLATZ Gene Family in Oryza Genus: Evolution, Expression During Inflorescence Development and Stress Responses

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 117 - 117

Published: Jan. 4, 2025

The PLATZ gene family, known for its pivotal roles in regulating plant growth, development, and stress responses, is of great significance rice biology crop improvement efforts. In this study, we undertook a comprehensive identification analysis the family across 10 Oryza genus species, including both cultivated wild varieties. A total 144 genes were identified, demonstrating their widespread distribution. Phylogenetic revealed six distinct groups among these genes, with high sequence similarity members indicating common evolutionary origin potential functional conservation. Further conserved motifs, domains, promoter regions provided insights into transcriptional regulation functions genes. Notably, expression profiling showed differential patterns specific such as OsPLATZ7, OsPLATZ9, OsPLATZ11, under various abiotic conditions hormone treatments, highlighting important adaptation signaling. Additionally, consistently OsPLATZ9 multiple tissues suggests involvement developmental processes. Overall, study provides detailed characterization rice, laying foundation future studies applications agricultural biotechnology.

Language: Английский

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Functional Characterization of CsBAS1, CsSND1, and CsIRX6 in Cucumber Defense Against Meloidogyne incognita

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2133 - 2133

Published: Feb. 27, 2025

Vascular tissue development plays a pivotal role in plant growth and defense against biotic stress. Root-knot nematodes, particularly Meloidogyne incognita (M. incognita), are globally distributed phytopathogens that cause severe economic losses variety of vascular plants. In this study, three bundle development-related genes, including CsBAS1, CsSND1, CsIRX6, were identified cucumber. Tissue-specific expression analysis revealed CsSND1 CsIRX6 highly expressed roots. Infection with M. showed dynamic changes for CsIRX6. Specially, upregulated at 14 days post-inoculation (dpi), while CsBAS1 was downregulated both 7 dpi dpi. Tissue localization studies using promoter-GUS constructs demonstrated pCsBAS1-GUS pCsSND1-GUS activity galls specific tissues, mRNA detected giant cells (GCs) situ methods. Virus-induced gene silencing (VIGS) their distinct roles nematode-induced gall formation. Silencing resulted increased root size, whereas led to reduced size. These findings highlight the functional significance cucumber incognita, offering insights into interplay between mechanisms.

Language: Английский

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Knock-Out of CmNAC-NOR Affects Melon Climacteric Fruit Ripening

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 10, 2022

Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6.3, involved climacteric regulation, has been found to be encoded by CmNAC-NOR , a homologue of the tomato NOR gene. To further investigate function, we obtained two CRISPR/Cas9-mediated mutants ( nor-3 and nor-1 ) Védrantais background. containing 3-bp deletion altering NAC domain A, resulted ~8 days delay without affecting In contrast, 1-bp fully disrupted domain, which completely blocked ripening. The fruits did not produce ethylene, no abscission layer was formed there external color change. Additionally, volatile components were dramatically altered, seeds well developed flesh firmness also altered. There with allele heterozygosis ~20 days. Our results provide new information regarding function melon ripening, suggesting it potential target for modulating shelf life commercial varieties.

Language: Английский

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The NAC transcription factors play core roles in flowering and ripening fundamental to fruit yield and quality

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 23, 2023

Fruits are derived from flowers and play an important role in human food, nutrition, health. In general, determine the crop yield, ripening affects fruit quality. Although transcription factors (TFs) only account for a small part of plant transcriptomes, they control global gene expression regulation. The plant-specific NAC (NAM, ATAF, CUC) TFs constitute large family evolving concurrently with transition both aquatic-to-terrestrial plants vegetative-to-reproductive growth. Thus, NACs yield quality by determining shoot apical meristem (SAM) inflorescence controlling ripening. present review focuses on various properties together their function regulation flower formation Hitherto, we have better understanding molecular mechanisms through abscisic acid (ABA) ethylene (ETH), but how regulate formation-related genes is largely unknown. future, should focus analysis redundancy identify pivotal regulators flowering potentially vital manipulation targets improving quantity

Language: Английский

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A dramatic decline in fruit citrate induced by mutagenesis of a NAC transcription factor, AcNAC1

Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21(8), P. 1695 - 1706

Published: May 10, 2023

Summary Citrate is a common primary metabolite which often characterizes fruit flavour. The key regulators of citrate accumulation in and vegetables are poorly understood. We systematically analysed the dynamic profiles organic acid components during development kiwifruit ( Actinidia spp.). continuously accumulated so that it became predominate contributor to total acidity at harvest. Based on co‐expression network analysis using different cultivars, an Al‐ACTIVATED MALATE TRANSPORTER gene AcALMT1 ) was identified as candidate responsible for accumulation. Electrophysiological assays expression this Xenopus oocytes revealed functions transporter. Additionally, transient overexpression significantly increased content, while tissues showing higher more citrate. highly correlated with 17 transcription factor candidates. However, dual‐luciferase EMSA indicated only NAC factor, AcNAC1, activated via direct binding its promoter. Targeted CRISPR‐Cas9‐induced mutagenesis AcNAC1 resulted dramatic declines levels malate quinate were not substantially affected. Our findings show transcriptional regulation major transporter, by kiwifruit, has broad implications other fruits vegetables.

Language: Английский

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A NAC transcription factor, PpNAC1, regulates the expression of PpMYB10.1 to promote anthocyanin biosynthesis in the leaves of peach trees in autumn

Horticulture Advances, Journal Year: 2023, Volume and Issue: 1(1)

Published: Sept. 30, 2023

Abstract Red leaves in autumn are characteristic of a very early-maturing ripening variety peach ( Prunus persica ). Analysis the genetic factors and molecular mechanisms associated with red-leaf phenotype can help breed early maturing varieties. This study investigated underlying red- green-leaf phenotypes autumn. compounds accumulated older but not younger extremely variety, ‘99-30-33’, medium-maturing ‘Zhongtao5 (CP5)’. Metabolic analysis showed that cyanidin-3- O -glucoside was most abundant anthocyanin red leaves. The segregation progenies obtained from crossing revealed unique hereditary phenomenon line Mendel’s law. BSA-seq RNA-seq analyses suggest PpNAC1 essential for enhancing biosynthesis highly upregulated than green Similar to , activator, PpMYB10.1 only gene expressed Moreover, functional genes involved biosynthesis, such as Flavonoid 3'-hydroxylase PpF3'H ), persicaDihydroflavonol reductase PpDFR Leucoantho-cyanidin dioxygenase Pp LDOX Glutathione S-transferase PpGST UDP-glucose flavonoid-3-O-glucosyltransferase PpUFGT were 99-30-33 downregulated CP5 Yeast one-hybrid dual-luciferase assays further confirmed which refers ' NAC NAM、A TAF1/2、CUC1/2 )' bound promoter stands MYB v-myb avian myeloblastosis viral ) 10.1 activated its expression along those PpUFGT. These results provide insights into responsible development color

Language: Английский

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