MaHDA6-MaNAC154 module regulates the transcription of cell wall modification genes during banana fruit ripening

Postharvest Biology and Technology, Journal Year: 2023, Volume and Issue: 198, P. 112237 - 112237

Published: Jan. 5, 2023

Language: Английский

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Polygalacturonase gene family analysis identifies FcPG12 as a key player in fig (Ficus carica L.) fruit softening

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: June 14, 2023

Abstract Background The fig ( Ficus carica L.) tree has high economic value. However, its fruit have a short shelf life due to rapid softening. Polygalacturonases (PGs) are essential hydrolases, responsible for the pectin degradation that plays key role in PG genes and their regulators not yet been characterized. Results In this study, 43 FcPG s were identified genome. They non-uniformly distributed on 13 chromosomes, tandem repeat gene clusters found chromosomes 4 5. Ka/Ks calculation collinear analysis indicated negative selection as main driver of family expansion. Fourteen expressed with FPKM values > 10, which seven positively correlated, three, negatively correlated Eleven upregulated two downregulated response ethephon treatment. FcPG12 , member cluster chromosome 4, was selected further analyses sharp increment transcript abundance during softening Transient overexpression led decreased firmness increased enzyme activity tissue. Two ethylene factor (ERF)-binding GCC-box sites promoter. Yeast one-hybrid dual luciferase assays showed FcERF5 binds directly promoter upregulates expression. expression, thereby increasing Conclusions Our study softening, direct positive regulation by . results provide new information molecular

Language: Английский

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MaMADS1–MaNAC083 transcriptional regulatory cascade regulates ethylene biosynthesis during banana fruit ripening

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(10)

Published: Sept. 8, 2023

Abstract The hormone ethylene is crucial in the regulation of ripening climacteric fruit, such as bananas. transcriptional biosynthesis throughout banana fruit has received much study, but cascaded machinery upstream regulators implicated pathway still poorly understood. Here we report that genes, including MaACS1, MaACO1, MaACO4, MaACO5, and MaACO8, were upregulated NAC (NAM, ATAF, CUC) transcription factor, MaNAC083, a ethylene-inhibited gene, was discovered potential binding protein to MaACS1 promoter by yeast one-hybrid screening. Further vitro vivo experiments indicated MaNAC083 bound directly promoters five thereby transcriptionally repressing their expression, which further verified transient overexpression experiments, where production inhibited through MaNAC083-modulated repression genes fruits. Strikingly, MaMADS1, ripening-induced MADS (MCM1, AGAMOUS, DEFICIENS, SRF4) found repress expression inhibiting trans-repression attenuating MaNAC083-repressed These findings collectively illustrated mechanistic basis MaMADS1–MaNAC083–MaACS1/MaACOs regulatory cascade controlling during ripening. increase our knowledge mechanisms at level are expected help develop molecular approaches control improve storability.

Language: Английский

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NAC transcription factor SlNOR-like1 plays a dual regulatory role in tomato fruit cuticle formation

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 75(7), P. 1903 - 1918

Published: Oct. 18, 2023

Abstract The plant cuticle is an important protective barrier on the surface, constructed mainly by polymerized cutin matrix and a complex wax mixture. Although pathway of biosynthesis has been clarified, knowledge transcriptional regulation network underlying fruit formation remains limited. In present work, we discovered that tomato fruits NAC transcription factor SlNOR-like1 knockout mutants (nor-like1) produced CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] displayed reduced deposition thickness, with microcracking phenotype, while accumulation was promoted. Further research revealed promotes binding to promoters glycerol-3-phosphate acyltransferase6 (SlGPAT6; key gene for monomer formation) CUTIN DEFICIENT2 (SlCD2; positive regulator production) activate their expression. Meanwhile, inhibits accumulation, acting as repressor targeting biosynthesis, transport-related genes 3-ketoacyl-CoA synthase1 (SlKCS1), ECERIFERUM 1-2 (SlCER1-2), SlWAX2, glycosylphosphatidylinositol-anchored lipid transfer 1-like (SlLTPG1-like). conclusion, executes dual regulatory effect development. Our results provide new model formation.

Language: Английский

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Ethylene induced AcNAC3 and AcNAC4 take part in ethylene synthesis through mediating AcACO1 during kiwifruit (Actinidia chinensis) ripening

Journal of the Science of Food and Agriculture, Journal Year: 2024, Volume and Issue: 104(12), P. 7367 - 7374

Published: April 25, 2024

Abstract BACKGROUND Ethylene plays a vital role in the ripening process of kiwifruit. A terrific amount transcription factors (TFs) have been shown to regulate ethylene synthesis various fruits. RESULTS In this research, two new NAC TFs, named AcNAC3 and AcNAC4, were isolated from kiwifruit, which belonged NAM subfamily. Bioinformatics analysis showed that both AcNAC4 hydrophilic proteins with similar three‐dimensional structures. The expression levels , AcACO1 increased during kiwifruit ripening, as well induced by repressed 1‐methylcyclopropene (1‐MCP). Correlation exhibited production was positively correlated . Moreover, acted transcriptional activators could bind activate promoter. CONCLUSION All results unveiled ethylene‐induced activators, might participate biosynthesis through activating providing insight synthetic regulation ripening. © 2024 Society Chemical Industry.

Language: Английский

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