Role of microRNAs in type 2 diseases and allergen-specific immunotherapy DOI Creative Commons
Constanze A. Jakwerth,

Hannah Kitzberger,

Dimitrii Pogorelov

et al.

Frontiers in Allergy, Journal Year: 2022, Volume and Issue: 3

Published: Sept. 12, 2022

MicroRNAs (miRs) have gained scientific attention due to their importance in the pathophysiology of allergic diseases as well potential biomarkers allergen-specific treatment options. Their function post-transcriptional regulators, controlling various cellular processes, is high since any single miR can target multiple mRNAs, often within same signalling pathway. MiRs alter dysregulated expression certain responses and contribute or cause, but some cases prevent repress, development diseases. In this review article, we describe current research on role specific miRs regulating immune epithelial cells specialized response stimuli, diseases, regulation therapeutic approach immunotherapy (AIT). Despite fact that AIT has been used successfully a causative option more than century, very little known about mechanisms its connections with microRNAs. order fill gap, aims provide an overview knowledge.

Language: Английский

Airway remodelling in asthma and the epithelium: on the edge of a new era DOI Creative Commons
Gilda Varricchi, Christopher E. Brightling, Christopher Grainge

et al.

European Respiratory Journal, Journal Year: 2024, Volume and Issue: 63(4), P. 2301619 - 2301619

Published: April 1, 2024

Asthma is a chronic, heterogeneous disease of the airways, often characterised by structural changes known collectively as airway remodelling. In response to environmental insults, including pathogens, allergens and pollutants, epithelium can initiate remodelling via an inflammatory cascade involving variety mediators that have downstream effects on both immune cells. These include epithelial cytokines thymic stromal lymphopoietin, interleukin (IL)-33 IL-25, which facilitate through cross-talk between cells fibroblasts, mast smooth muscle cells, well signalling with such macrophages. The also independently inflammation in mechanical stress present during bronchoconstriction. Furthermore, genetic epigenetic alterations components are believed influence Here, we review recent advances our understanding roles driving remodelling, facilitated developments sequencing imaging techniques. We explore how new existing therapeutics target could modify

Language: Английский

Citations

32
Mechanisms and Predictive Biomarkers of Allergen Immunotherapy in the Clinic DOI
Janice A. Layhadi, Anastasia Lalioti, Elizabeth Palmer

et al.

The Journal of Allergy and Clinical Immunology In Practice, Journal Year: 2023, Volume and Issue: 12(1), P. 59 - 66

Published: Nov. 22, 2023

Language: Английский

Citations

14
Association of endometriosis with asthma: a study of the NHANES database in 1999–2006 DOI Creative Commons
Guangxin Pan, Pei Zhang, Sha Li

et al.

Journal of Health Population and Nutrition, Journal Year: 2024, Volume and Issue: 43(1)

Published: April 9, 2024

Abstract Objective Asthma is a chronic inflammatory disease of the airways with gender differences in prevalence after puberty. Recent studies have reported relationship between asthma and endometriosis, possibly related to immune response mechanisms, but evidences are limited inconsistent. Herein, this research aimed investigate association endometriosis based on representative population United States (U.S.) provide some reference for further exploration mechanism difference asthma. Methods In cross-sectional study, data women aged ≥ 20 years old were extracted from National Health Nutrition Examination Survey (NHANES) database 1999–2006. Weighted univariate multivariate logistic regression analyses utilized explore The models adjusted covariates including age, race, education level, marital status, poverty income ratio (PIR), body mass index (BMI), waist circumference, smoking, estrogen progesterone hormones use, uterine fibroids, at least one ovary removed, birth control pills intake. evaluation indexes odds ratios (ORs) 95% confidence intervals (CIs). Subgroup BMI, pregnancy history also performed. Results Among 5,556 eligible women, 782 had asthma, 380 endometriosis. average age participants was 37.19 old, more than half them non-Hispanic White (68.44%). After adjusting covariates, associated higher compared non-endometriosis [OR = 1.48, 95%CI: (1.10–1.99)]. This found 40–49 2.26, (1.21–4.23)], BMI 25-29.9 kg/m 2 2.87, (1.52–5.44)], 1.44, (1.01–2.06)] subgroups. Conclusion Endometriosis positive adult women. Females should take care about monitoring reduce potential risk Further still needed clarify causal

Language: Английский

Citations

5
Epithelium‐derived cystatin SN inhibits house dust mite protease activity in allergic asthma DOI
Lei Yao,

Xijing Yuan,

Heng Fu

et al.

Allergy, Journal Year: 2023, Volume and Issue: 78(6), P. 1507 - 1523

Published: April 7, 2023

Abstract Background Allergen source‐derived proteases are a critical factor in the formation and development of asthma. The cysteine protease activity house dust mite (HDM) disrupts epithelial barrier function. expression cystatin SN (CST1) is elevated asthma epithelium. CST1 inhibits activity. We aimed to elucidate role epithelium‐derived caused by HDM. Methods protein levels sputum supernatants serum patients with healthy volunteers were measured ELISA. ability suppress HDM‐induced bronchial function was examined vitro. effects exogenous on abrogating inflammation mice vivo. Results higher (142.4 ± 8.95 vs 38.87 6.85 ng/mL, P < 0.0001) (1129 73.82 703.1 57.02 pg/mL, = 0.0035) than subjects. significantly not well‐ very poorly controlled those well‐controlled Sputum negatively correlated lung lower HDM‐specific IgE (sIgE)‐positive asthmatics sIgE‐negative asthmatics. disruption suppressed recombinant human (rhCST1) vitro Conclusion Our data indicated that suppresses symptoms protecting asthmatic through inhibiting allergenic may serve as potential biomarker for control.

Language: Английский

Citations

12
Neuropeptide S and its receptor aggravated asthma via TFEB dependent autophagy in bronchial epithelial cells DOI Creative Commons
Zhixu Wang, Peng Zhao, Gen Yan

et al.

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 10, 2025

Asthma is a prevalent respiratory disorder with limited treatment strategy. Neuropeptide S (NPS) highly conserved peptide via binding to its receptor NPSR, susceptibility gene for asthma from genomics studies. However, little known about the role of NPS-NPSR in pathogenesis asthma. This study was performed determine effect and underlying mechanism on NPSR knockdown verified affect through autophagy by transcriptome sequencing molecular biology experiments animal models. Silencing transcription factor EB bronchial epithelial cell line validation activation dependent EB. Our results showed that expression markedly increased asthmatic humans mice, mainly localized cells. Using ovalbumin (OVA) papain-induced mouse models, NPSR-deficient mice exhibited significantly alleviated asthma, reduced small airway lesions inflammatory infiltration compared wild-type mice. OVA papain promoted TFEB-mediated ATG5 LC3 II expression, NPS effectively regulated TFEB autophagy. In turn, specific could restore exogenous antagonist cytokines secretion Furthermore, Prkcg may be key upstream targeting TFEB-autophagy pathway involved exacerbated regulating axis injury, which potential target therapy.

Language: Английский

Citations

0
Airway epithelial cells as drivers of severe asthma pathogenesis DOI Creative Commons
Delbert R. Dorscheid, Gail M. Gauvreau, Steve N. Georas

et al.

Mucosal Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Our understanding of the airway epithelium's role in driving asthma pathogenesis has evolved over time. From being regarded primarily as a physical barrier that could be damaged via inflammation, epithelium is now known to actively contribute development through interactions with immune system. The contains multiple cell types specialized functions spanning action, mucociliary clearance, recruitment, and maintenance tissue homeostasis. Environmental insults may cause direct or indirect injury leading impaired function, epithelial remodelling increased release inflammatory mediators. In severe asthma, repair process inhibited response exaggerated, downstream inflammation. Genetic epigenetic mechanisms also maintain dysregulation barrier, adding disease chronicity. Here, we review how targeting can treatment.

Language: Английский

Citations

0
Altered ontogeny and transcriptomic signatures of tissue-resident pulmonary interstitial macrophages ameliorate allergic airway hyperresponsiveness DOI Creative Commons
Robert M. Tighe, Anastasiya Birukova,

Yuryi Malakhau

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 25, 2024

Introduction Environmental exposures and experimental manipulations can alter the ontogenetic composition of tissue-resident macrophages. However, impact these alterations on subsequent immune responses, particularly in allergic airway diseases, remains poorly understood. This study aims to elucidate significance modified macrophage ontogeny resulting from environmental responses house dust mite (HDM) allergen. Methods We utilized embryonic lineage labeling delineate profile macrophages at baseline following resolution repeated lipopolysaccharide (LPS)-induced lung injury. investigated differences (HDM)-induced allergy assess influence responses. Additionally, we employed single-cell RNA sequencing (scRNAseq) immunofluorescent staining characterize pulmonary composition, associated pathways, tissue localization. Results Our findings demonstrate that homeostatic alveolar interstitial is altered after LPS-induced injury, leading replacement embryonic-derived by bone marrow-derived shift with reduced HDM-induced Through scRNAseq staining, identified a distinct subset resident-derived expressing genes localized adjacent terminal bronchi, diminished prior LPS exposure. Discussion These results suggest pivotal role for modulating Moreover, our highlight implications shaping future influencing development allergies. By elucidating mechanisms underlying phenomena, this provides valuable insights into potential therapeutic targets diseases avenues further research modulation disease prevention.

Language: Английский

Citations

3
Exosomal miR-129-2-3p promotes airway epithelial barrier disruption in PM2.5-aggravated asthma DOI
Caihong Wang,

Zeyu Niu,

Yan Zhang

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 370, P. 123053 - 123053

Published: Oct. 30, 2024

Citations

3
Unraveling genetic threads: Identifying novel therapeutic targets for allergic rhinitis through Mendelian randomization DOI Creative Commons
Xuerong Huang,

Ruoyi Shen,

Z. Zheng

et al.

World Allergy Organization Journal, Journal Year: 2024, Volume and Issue: 17(7), P. 100927 - 100927

Published: July 1, 2024

BackgroundAllergic rhinitis (AR) is a pervasive global health issue, and currently, there scarcity of targeted drug therapies available. This study aims to identify potential druggable target genes for AR using Mendelian randomization (MR) analysis.MethodsMR analysis was conducted assess the causal effect expression quantitative trait loci (eQTL) in blood on AR. Data were collected from 2 datasets: FinnGen(R9) (11,009 cases 359,149 controls) UK Biobank (25,486 87,097 controls). Colocalization utilized common genetic variations between identified We also employed available genome-wide association studies (GWAS) data gauge impact biomarkers other allergic diseases.ResultsThis employs MR analyze relationship 3410 After Bonferroni correction, 10 found be significantly associated with risk (P < 0.05/3410). revealed significant variation CFL1 EFEMP2 AR, sharing direct variants (colocalization probability PP.H3 + PP.H4 > 0.8), highlighting their importance as therapeutic targets The gene showed link levels thymic stromal lymphopoietin (TSLP), eosinophil count, interleukin-13 (IL-13) = 0.016, 7.45E-16, 0.00091, respectively). causally related IL-13, interleukin-17 (IL-17) 0.00012, 0.032, PheWAS associations asthma, whereas both asthma eczema. Protein-Protein Interaction (PPI) network further unveiled interactions proteins immune regulation inflammatory responses, 77.64% consisting bindings, indicating key roles modulating AR-related responses. Notably, an 8.01% correlation immune-related pathways involved responses.ConclusionThese present notable autoimmune diseases, offering valuable developing new therapies.

Language: Английский

Citations

2
New insights into inflammatory memory of epidermal stem cells DOI Creative Commons

Dapeng Cheng,

Xiaochen Zhu,

Shaochen Yan

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 31, 2023

Inflammatory memory, as one form of innate immune has a wide range manifestations, and its occurrence is related to cell epigenetic modification or metabolic transformation. When re-encountering similar stimuli, executing cells with inflammatory memory function show enhanced tolerated response. Studies have identified that not only hematopoietic stem fibroblasts effects, but also from various barrier epithelial tissues generate maintain memory. Epidermal cells, especially hair follicle play an essential role in wound healing, immune-related skin diseases, cancer development. In recent years, it been found epidermal can remember the response implement more rapid subsequent stimuli. This review updates advances focuses on mechanisms cells. We are finally looking forward further research which will allow for development precise strategies manipulate host responses infection, injury, disease.

Language: Английский

Citations

6