Masculinizer Gene Controls Male Sex Determination in the Codling Moth, Cydia Pomonella DOI
Kristýna Pospíšilová, Arjèn E. van’t Hof, Atsuo Yoshido

et al.

Published: Jan. 1, 2023

The molecular mechanisms of sex determination in moths and butterflies (Lepidoptera) with female heterogamety (WZ/ZZ) are poorly understood, except the silkworm Bombyx mori. However, Masculinizer (Masc) gene that controls male development dosage compensation B. mori, appears to be conserved Lepidoptera, as its masculinizing function was recently confirmed several moth species. In this work, we investigated role Masc codling Cydia pomonella (Tortricidae), a globally important pest pome fruits walnuts. structure C. ortholog, CpMasc, is similar mori Masc. unlike identified 14 splice variants CpMasc available transcriptomes. Subsequent screening for specificity genetic variation using publicly data RT-PCR revealed three male-specific variants. Then qPCR analysis these sex-specific expression showing peak only early embryos. Knockdown by RNAi during embryogenesis resulted shift from male- female-specific splicing doublesex (Cpdsx) gene, downstream effector, ZZ embryos, leading strongly female-biased ratio. These clearly demonstrate functions sex-determining cascade pomonella. Our study also showed transcripts provided maternally, they were detected unfertilized eggs after oviposition mature dissected virgin females. This finding unique, maternal provision mRNA has rarely been studied Lepidoptera.

Language: Английский

Diverse Molecular Mechanisms Underlying Microbe-Inducing Male Killing in the Moth Homona magnanima DOI Creative Commons
Hiroshi Arai,

Takumi Takamatsu,

Shiou-Ruei Lin

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: unknown

Published: April 26, 2023

Many microbes induce male killing (MK) in various insect species. However, it is not well understood whether adopt similar or different MK mechanisms.

Language: Английский

Citations

30
Genome editing in pests: basic science to applications DOI
Xien Chen, Subba Reddy Palli

Journal of Pest Science, Journal Year: 2024, Volume and Issue: 97(3), P. 1135 - 1152

Published: Jan. 24, 2024

Language: Английский

Citations

7
Sex determination in moths and butterflies: Masculinizer as key player DOI Creative Commons
Arjèn E. van’t Hof, Atsuo Yoshido, František Marec

et al.

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 101375 - 101375

Published: April 1, 2025

Language: Английский

Citations

0
Current status and research progress of sugarcane stem borers management DOI
Jinda Wang, Haowei Lin, Shang XianKun

et al.

European Journal of Agronomy, Journal Year: 2025, Volume and Issue: 168, P. 127644 - 127644

Published: April 22, 2025

Language: Английский

Citations

0
Masculinizer is not post-transcriptionally regulated by female-specific piRNAs during sex determination in the Asian corn borer, Ostrinia furnacalis DOI Creative Commons
Takahiro Fukui, Keisuke Shoji, Takashi Kiuchi

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 156, P. 103946 - 103946

Published: April 17, 2023

Language: Английский

Citations

7
CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Spodoptera frugiperda): Progress and Prospects DOI Creative Commons

Yussuf Mohamed Salum,

Anyuan Yin,

Uroosa Zaheer

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1074 - 1074

Published: Aug. 27, 2024

The fall armyworm (Spodoptera frugiperda) poses a substantial threat to many important crops worldwide, emphasizing the need develop and implement advanced technologies for effective pest control. CRISPR/Cas9, derived from bacterial adaptive immune system, is prominent tool used genome editing in living organisms. Due its high specificity adaptability, CRISPR/Cas9 system has been various functional gene studies through knockout applied research engineer phenotypes that may cause economical losses. practical application of diverse insect orders also provided opportunities developing strategies genetic control, such as drive precision-guided sterile technique (pgSIT). In this review, comprehensive overview recent progress S. frugiperda presented. We outline fundamental principles applying embryonic microinjection highlight study genes associated with biological aspects, including body color, insecticide resistance, olfactory behavior, sex determination, development, RNAi. ability technology induce sterility, disrupt developmental stages, influence mating behaviors illustrates roles management strategies. Furthermore, review addresses limitations studying function explores future potential promising controlling pest.

Language: Английский

Citations

2
Understanding the genetics of sex determination in insects and its relevance to genetic pest management DOI Creative Commons
Jade S. Ashmore, Bernard Slippers, Tuan A. Duong

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Sex determination pathways regulate male and female‐specific development differentiation offer potential targets for genetic pest management methods. Insect sex are comprised of primary signals, relay genes terminal genes. Primary signals coleopteran, dipteran, hymenopteran lepidopteran species highly diverse the sex‐specific splicing based on signal dosage, amino acid composition or interaction with paternally inherited In coleopterans, hymenopterans some dipterans, Transformer orthologs from serine‐arginine protein family that Alternative in dipterans lack lepidopterans. Doublesex Fruitless conserved zinc‐finger proteins expression downstream influencing physical traits courtship behaviours a manner. Genetic methods can use different mechanisms to exploit disrupt regions Female‐specific be exploited produce lethal gene only females disrupted impede female fertility. Reducing number fertile populations creates male‐biased ratio eventually leads local elimination population. Knowledge basis is important enable these management.

Language: Английский

Citations

2
CRISPR/Cas9-Mediated Mutagenesis of Sex-Specific Doublesex Splicing Variants Leads to Sterility in Spodoptera frugiperda, a Global Invasive Pest DOI Creative Commons

Junwen Gu,

Jingyi Wang, Honglun Bi

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(22), P. 3557 - 3557

Published: Nov. 10, 2022

Spodoptera frugiperda (J. E. Smith), an emerging invasive pest worldwide, has posed a serious agricultural threat to the newly invaded areas. Although somatic sex differentiation is fundamentally conserved among insects, determination cascade in S. largely unknown. In this study, we cloned and functionally characterized Doublesex (dsx), “molecular switch” modulating sexual dimorphism using male- female-specific isoforms. Given that Lepidoptera recalcitrant RNAi, CRISPR/Cas9-mediated mutagenesis was employed construct mutants. Specifically, designed target sites on exons 2, 4, 5 eliminate common, female-specific, male-specific regions of dsx (Sfdsx), respectively. As expected, abnormal development both external internal genitalia observed during pupal adult stages. Interestingly, knocking out sex-specific variants led significantly reduced fecundity fertility adults corresponding sex. Our combined results not only confirm function but also provide empirical evidence for as potential Sterile Insect Technique (SIT) combat globally sustainable environmentally friendly way.

Language: Английский

Citations

11
Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella DOI
Kristýna Pospíšilová, Arjèn E. van’t Hof, Atsuo Yoshido

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 160, P. 103991 - 103991

Published: Aug. 1, 2023

Language: Английский

Citations

6
Knockout of ovary serine protease Leads to Ovary Deformation and Female Sterility in the Asian Corn Borer, Ostrinia furnacalis DOI Open Access

Porui Zhang,

Zuerdong Jialaliding,

Junwen Gu

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16311 - 16311

Published: Nov. 14, 2023

Oogenesis in insects is a carefully orchestrated process, facilitating the formation of female gametes, which regulated by multiple extrinsic and intrinsic factors, including ovary serine protease (Osp). As member family, Osp homolog Nudel, maternally required defining embryonic dorsoventral polarity Drosophila. In this study, we used CRISPR/Cas9-mediated mutagenesis to functionally characterize Asian corn borer, Ostrinia furnacalis, devastating maize pest throughout Asia Australia. Building on previous knowledge, hypothesized that knockout would disrupt development O. furnacalis females. To examine overarching hypothesis, (1) cloned characterized from (2) designed target sites exons 1 4 construct CRISPR/Cas9 system, (3) documented phenotypic impacts among mutants. result, examined temporal-spatial expression profiles OfOsp, has an open reading frame 5648 bp length encodes protein 1873 amino acids; established mutants; recessive, female-specific sterility OfOspF mutants, absent or deformed oviducts reduced fertility but not male Overall, combined results support our initial hypothesis for development, specifically ovarian maturation, Given its substantial sterility, provides potential Sterile Insect Technique (SIT) manage Lepidoptera pests general species complex particular.

Language: Английский

Citations

5