Heliyon,
Journal Year:
2024,
Volume and Issue:
10(14), P. e34651 - e34651
Published: July 1, 2024
Based
on
scientific
evidence,
it
seems
that
bio-magnetic
systems
can
change
the
process
of
cancer
cell
death
by
affecting
distribution
pressure
and
mechanical
stress
in
tumor
tissue.
Already
most
research
has
been
done
experimentally
few
mathematical
modeling
numerical
simulations
have
to
investigate
relationship
between
magnetic
parameters
This
is
despite
fact
order
be
able
make
new
equipment
with
help
medical
engineering
methods,
definitely
necessary
mathematics
governing
problem
changes
effective
(such
as
shape
source,
flux
density,
source
distance
ferro-fluid
volume
fraction)
should
studied
much
possible.
In
this
research,
using
simulation
modeling,
four
common
geometrical
shapes
(rectangular
circular)
static
field
were
used
created
The
results
showed
when
density
fraction
also
magnet
tissue
are
kept
constant,
well
without
spending
any
extra
energy,
for
a
rectangular
magnet,
just
changing
way
placed
tissue,
average
biomechanical
inside
causes
25
%
change.
Also,
circular
doubling
radius
73
Current Issues in Molecular Biology,
Journal Year:
2025,
Volume and Issue:
47(3), P. 147 - 147
Published: Feb. 25, 2025
Glaucoma
is
a
leading
cause
of
irreversible
blindness,
with
elevated
intraocular
pressure
(IOP)
as
the
most
important
risk
factor.
The
trabecular
meshwork
(TM)
and
Schlemm’s
canal
are
main
components
proximal
aqueous
humor
outflow
pathway.
Their
dysfunction
major
contributor
to
IOP
elevation.
This
study
aims
identify
validate
key
biomarkers
for
TM
endothelial
(SCE)
cells.
A
Microarray
was
performed
on
characterized
human
SCE
cells
analyze
their
transcriptome
profiling.
Differentially
expressed
genes
(DEGs)
were
identified
cross-referenced
published
single-cell
RNA
sequencing
(scRNA-Seq)
datasets
ensure
cell-specific
relevance.
Further
validation
using
qPCR
re-confirmed
scRNA-seq
datasets.
One-way
ANOVA
used
statistical
analysis,
p
<
0.05
considered
significant.
revealed
341
DEGs,
enriched
in
metabolic
signaling
pathways
adhesion,
immune,
morphogenesis-related
processes.
Cross-referencing
scRNA-Seq
data
refined
list
candidate
biomarkers,
confirmed
significant
gene
expression
differences
between
CTTNBP2
MGARP
cell
markers.
JAM2,
PODXL,
IFI27
new
biomarkers.
validated
offer
insights
into
glaucoma
pathophysiology
lay
groundwork
targeted
therapies.
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(3), P. 389 - 389
Published: March 8, 2025
This
study
evaluated
the
effects
of
α2-adrenergic
agonist,
prostaglandin
F2α
analog,
and
EP2
receptor
agonist
on
tunicamycin-induced
endoplasmic
reticulum
(ER)
stress
fibrosis
in
human
trabecular
meshwork
(TM)
cells.
Human
TM
cells
were
treated
with
tunicamycin
for
24
h,
followed
by
cotreatment
brimonidine
(BRI),
latanoprost
(LAT),
or
omidenepag
(OMD).
Immunocytochemistry
was
used
to
assess
expressions
collagen
type
I
alpha
1
chain
(COL1A1),
fibronectin,
F-actin,
alpha-smooth
muscle
actin
(α-SMA).
Western
blotting
performed
evaluate
levels
C/EBP
homologous
protein
(CHOP),
78-kDa
glucose-regulated
(GRP78),
splicing
X-box
binding
protein-1
(sXBP-1).
Real-time
qPCR
examine
mRNA
COL1A1,
connective
tissue
growth
factor
(CTGF),
α-SMA,
CHOP,
GRP78,
sXBP-1.
Expressions
CTGF,
sXBP-1
significantly
increased
after
treatment.
BRI
downregulated
LAT
OMD
reduced
CHOP
compared
tunicamycin-treated
group.
BRI,
LAT,
attenuated
cellular
cytoskeletal
changes
increase
markers
such
as
α-SMA.
In
addition,
COL1A1
expression
lowered
BRI-cotreated
Cotreatment
alleviates
ER
Bioengineering,
Journal Year:
2023,
Volume and Issue:
10(9), P. 1038 - 1038
Published: Sept. 4, 2023
Background:
More
than
~70%
of
the
aqueous
humor
exits
eye
through
conventional
outflow
pathway
that
is
comprised
trabecular
meshwork
(TM),
juxtacanalicular
tissue
(JCT),
inner
wall
endothelium
Schlemm’s
canal
(SC).
The
flow
resistance
in
JCT
and
SC
basement
membrane
thought
to
play
an
important
role
regulation
intraocular
pressure
(IOP)
eye,
but
current
imaging
techniques
do
not
provide
enough
information
about
mechanics
these
tissues
or
this
area.
Methods:
A
normal
human
was
perfusion-fixed
a
radial
wedge
TM
from
high-flow
region
dissected.
were
then
sliced
imaged
using
serial
block-face
scanning
electron
microscopy.
Slices
images
selected
segmented
create
3D
finite
element
model
cells
with
membrane.
used
replace
intertrabecular
spaces,
pores,
giant
vacuoles,
fluid–structure
interaction
employed
couple
motion
humor.
Results:
Higher
tensile
stresses
(0.8-kPa)
strains
(25%)
observed
beneath
vacuoles
open
pores.
volumetric
average
shear
stress
higher
JCT/SC.
As
approached
SC,
velocity
decreased,
resulting
formation
small
eddies
immediately
after
left
wall.
Conclusions:
Improved
modeling
can
enhance
our
understanding
funneling.
Serial
microscopy
achieve
this,
micro-segmental
patterns
ex
vivo
perfused
eyes
suggest
hypothetical
mechanism.
Reviews in the Neurosciences,
Journal Year:
2024,
Volume and Issue:
35(8), P. 839 - 853
Published: July 22, 2024
Abstract
Central
nervous
system
(CNS)
diseases,
such
as
stroke,
traumatic
brain
injury,
dementia,
and
demyelinating
are
generally
characterized
by
high
morbidity
mortality,
which
impose
a
heavy
economic
burden
on
patients
their
caregivers
throughout
lives
well
public
health.
The
occurrence
development
of
CNS
diseases
closely
associated
with
series
pathophysiological
changes
including
inflammation,
blood–brain
barrier
disruption,
abnormal
coagulation.
Endothelial
glycocalyx
(EG)
plays
key
role
in
these
changes,
making
it
novel
intervention
target
for
diseases.
Herein,
we
review
the
current
understanding
EG
common
from
perspective
individual
pathways/cytokines
systematic
processes.
Furthermore,
emphasize
recent
developments
therapeutic
agents
targeted
toward
protection
or
restoration
EG.
Some
treatments
have
yielded
unexpected
pharmacological
results,
previously
unknown
mechanisms
underlying
degradation
destruction
has
been
brought
to
light.
anti-inflammatory,
anticoagulative,
antioxidation
effects
its
protective
exerted
via
recognized.
