Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 35
Published: Jan. 1, 2023
Language: Английский
Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 249 - 265
Published: Jan. 1, 2025
Language: Английский
Citations
0
Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101125 - 101125
Published: June 13, 2024
Extracellular matrices (ECMs) play a key role in nerve repair and are recognized as the natural source of biomaterials. In parallel to extensively studied tissue-derived ECMs (ts-ECMs), cell-derived (cd-ECMs) also have capability partially recapitulate complicated regenerative microenvironment native tissues. Notably, cd-ECMs can avoid shortcomings ts-ECMs. Cd-ECMs be prepared by culturing various cells or even autologous vitro under pathogen-free conditions. And mild decellularization achieve efficient removal immunogenic components cd-ECMs. Moreover, more readily customizable desired functional properties. These advantages garnered significant attention for potential neuroregenerative medicine. As promising biomaterials, bring new hope effective treatment peripheral injuries. Herein, this review comprehensively examines current knowledge about characteristics their mechanisms interaction with regeneration, particular focus on preparation, engineering optimization, scalability The applications from distinct cell sources reported tissue highlighted summarized. Furthermore, limitations that should addressed outlooks related clinical translation put forward well.
Language: Английский
Citations
3
Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11
Published: Aug. 10, 2023
Purpose: Obtaining sufficient numbers of cells in a short time is major goal cell culturing therapy and tissue engineering. However, current bidimensional (2D) culture methods are associated to several limitations, including low efficiency the loss key differentiation markers on cultured cells. Methods: In present work, we have designed novel biofabrication method based three-dimensional (3D) system (FIBRIAGAR-3D). Human Wharton’s jelly mesenchymal stromal (HWJSC) were 3D using 100%, 75%, 50%, 25% concentrations fibrin-agarose biomaterials (FA100, FA75, FA50 FA25 group) compared with control classical 2D systems (CTR-2D). Results: Our results showed significant increase number generated after 7 days culture, displaying numerous expansions towards biomaterial, overexpression proliferation marker KI67 was found for FA75 FA100 groups. TUNEL qRT-PCR analyses demonstrated that use FIBRIAGAR-3D not an induction apoptosis by Instead, retained expression typical phenotypic HWJSC, CD73, CD90, CD105, NANOG OCT4, biosynthesis such as types-I IV collagens, some these markers, especially group. Finally, our analysis 8 signaling molecules revealed decrease GM-CSF, IFN-g, IL2, IL4, IL6, IL8, TNFα, suggesting did induce pro-inflammatory molecules. Conclusion: These confirm usefulness without altering phenotype immunogenicity opens door possibility this engineering human cornea, oral mucosa, skin, urethra, among other structures.
Language: Английский
Citations
4
Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 1011 - 1045
Published: Jan. 1, 2024
Language: Английский
Citations
0
Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 35
Published: Jan. 1, 2023
Language: Английский
Citations
1