Oral cell lysates reduce osteoclastogenesis in murine bone marrow cultures DOI Creative Commons
Layla Panahipour,

Azarakhsh Oladzad Abbasabadi,

Feng Shao

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 19, 2023

Abstract Mechanical and thermal cell damage can occur as a consequence of invasive procedures related to drilling, the insertion dental implants, well periodontal treatments. Necrotic cells release content their cytoplasm membrane fragments thereby signaling need for repair, process that includes bone resorption by osteoclasts inflammation. Here we screened lysates from human gingival fibroblasts, HSC2 TR146 oral squamous carcinoma lines, murine IDG-SW3 osteocytic RAW264.7 macrophage lines potential modulate in vitro osteoclastogenesis marrow cultures. We also tested impact necrotic expression inflammatory cues ST2 stromal cells. report here independent or origin, all significantly reduced cultures; indicated osteoclast marker genes cathepsin K tartrate-resistant acid phosphatase, respective histochemical staining multinucleated found greatly pushed CCL2, CCL5, CXCL1, IL1, IL6 These findings suggest reduce but only damaged force produce an environment.

Language: Английский

The role and mechanism of platelet-rich fibrin in alveolar bone regeneration DOI Open Access

Ming Liu,

Yu Liu, Feng Luo

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 168, P. 115795 - 115795

Published: Nov. 2, 2023

Platelet-rich fibrin (PRF), as an autologous blood preparation, has been receiving increasing attention in recent years and successfully applied various clinical treatments for alveolar bone regeneration the oral field. This review focuses on analyzing summarizing role mechanism of PRF regeneration. We first provide a brief introduction to PRF, then summarize mechanisms by which promotes from three aspects: osteogenesis mechanism, induction conduction involving multiple signaling pathways such Smad, ERK1/2, PI3K/Akt, Wnt/β-catenin. also explore roles scaffold, filler, combination with graft materials, detailing how provides wealth experimental evidence. Finally, we current applications fields. The is becoming increasingly important, its are more research understanding. article will reference significant value related exploration may lead discovery new therapeutic targets development effective efficient treatment strategies.

Language: Английский

Citations

9
RNAseq of Gingival Fibroblasts Exposed to PRF Membrane Lysates and PRF Serum DOI Creative Commons

Atefe Imani,

Layla Panahipour, Hannes Kühtreiber

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(15), P. 1308 - 1308

Published: Aug. 5, 2024

Platelet-rich fibrin (PRF) is prepared by spontaneous coagulation of fractionated blood. When squeezed between two plates, PRF separated into solid membranes and a liquid exudate, the serum. The question arises regarding how much overall activity remains in what discarded To this end, we have exposed gingival fibroblasts to lysates from serum, followed bulk RNA sequencing. A total 268 up- 136 down-regulated genes membrane were significantly regulated under premise minimum log2 with 2.5-fold change minus log10 significance level two, respectively. serum only caused 62 32 these conditions. Among 46 commonly up-regulated CXCL1, CXCL5, CXCL6, CXCL8, IL33, IL6, PTGS2/COX2, stanniocalcin-1—all linked an inflammatory response. further increased chemokines CCL2, CCL7, CXCL2, CXCL3, IL1R1, IL1RL1, IL1RN, as well paracrine factors IL11, LIF, IGF1, BMP2, BMP6, FGF2, CCN2/CTGF, all hyaluronan synthases. On other hand, DKK1. included interferon-induced protein tetratricopeptide repeats (IFIT1, IFIT2, IFIT3) odd-skipped-related transcription (OSR1 OSR2), FGF18 GDF15, Taken together, lysates, compared cause more complex response fibroblasts, but each chemokine expression fibroblasts.

Language: Английский

Citations

3
PRF Lysates Enhance the Proliferation and Migration of Oral Squamous Carcinoma Cell Lines DOI Creative Commons
Layla Panahipour, Rebecca Croci, Sara Guarnieri

et al.

Dentistry Journal, Journal Year: 2023, Volume and Issue: 11(10), P. 242 - 242

Published: Oct. 19, 2023

Platelet-rich fibrin (PRF) is an autologous fibrin-rich matrix where activated platelets and leucocytes accumulate. PRF has a wide spectrum of clinical indications with the overall aim supporting tissue regeneration which in dentistry includes healing healthy oral mucosa epithelial cells. In squamous cell carcinoma lesions, however, cells undergo malignant transformation, indicated by their unrestricted proliferation migration potential, should not be further enhanced wound-healing formula. Yet, little known about how carcinomas respond to lysates. The present study was, therefore, test capacity lysates change transcriptome HSC2 perform bioassays support findings. Based on RNAseq analysis, caused increase genes functionally linked replication migration. this screening approach, cells, as 3[H]-thymidine incorporation, counting, expression proliferation-related genes. Moreover, sped up scratch assay requiring actin polymerization. Taken together, our data showing that are mitogenic stimulate motility lines could indication treatment cases carefully considered.

Language: Английский

Citations

2
Regenerative Effect of Lyophilized Mesenchymal Stem Cells Combined with Platelet-Rich Fibrin in a Skin Wound Model in Geriatric and Young Rats DOI Creative Commons

Osman Bulut,

Deniz Genç, Çiğdem Elif Demirci Dönmez

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 26, 2024

Abstract The aim of this study was to investigate the regenerative effect lyophilized dental follicle mesenchymal stem cells (DF-MSCs) combined with platelet-rich fibrin (PRF) on geriatric skin wounds. Human DF-MSCs isolated from healthy donors and PRF were mixed incubated in a 37°C incubator for 1–2 hours containing 1 million 150 mg PRF. mixture suspended freeze-drying solution then lyophilized. Wounds created back Wistar albino rats using 6 mm punch. Lyophilized DF-MSCs, PRF, or + applied wounds rats. On 15th day, wound area histopathologically evaluated Peripheral blood samples analyzed total reactive oxygen species (TAOS), inflammatory cytokine levels ELISA. In both young treated began started significantly decrease 10th day compared untreated group (p < 0.05). Histopathological examination revealed that groups, epithelial integrity scarless healing increased groups There no significant differences TAOS, oxidant state (TOS), tumor necrosis factor (TNF), interleukin-6 (IL6), hydroxyproline serum 0.005). rats, (HYPS) DF-MSC 0.01), IL6 control 0.01). which is shelf-stable ready-to-use product, hold promise, especially traumatic individuals longer times.

Language: Английский

Citations

0
Expression of wound healing markers in gingival crevicular fluid following root-coverage procedures: A systematic review of randomized clinical trials DOI
Vikender Singh Yadav, Kanika Makker, Nitesh Tewari

et al.

Archives of Oral Biology, Journal Year: 2024, Volume and Issue: 166, P. 106035 - 106035

Published: July 1, 2024

Language: Английский

Citations

0
Aquisição de medicamentos no setor público de saúde: revisão de escopo DOI Creative Commons
Fernando Rodrígues de Oliveira, Letícia Fracaro,

Dualia Ledur Bona

et al.

Caderno Pedagógico, Journal Year: 2024, Volume and Issue: 21(12), P. e10299 - e10299

Published: Nov. 7, 2024

Cartilage has limited self-repair ability due to its complex structure and chondrocytes' relatively low metabolic activities. Functional repair of articular cartilage defects is a significant challenge for tissue engineering. Mesenchymal stromal cells (MSC) platelet-rich fibrin membrane (PRFM) in repairing damaged rabbit. They were subjected osteochondral lesion randomly into five groups: Group A, evaluated immediately after injury; B, natural evolution the C, MSC; D, PRFM E, MSC+ PRFM. Animals A euthanized removing cartilaginous flap, while animals Groups E 60 days. The regeneration was by analyzing residual extension, as well density chondrocytes chondroblasts regenerated adjacent lesion. Results, (MSC + PRFM) showed greater reduction size compared other groups. Our results indicate that combination MSC can successfully resurface defect with restore subchondral bone specie studied, being effective reducing hyaline defects; however, products might be more or less appropriate treating different types tissues pathologies. clinical efficacy PRP remains under debate. Therefore, further research needed at both basic science levels.

Language: Английский

Citations

0
Platelet-rich fibrin: Its role in curbing the inflammation and improvement of the wound-healing process DOI Creative Commons
Zohreh Jadali, Farideh Doroodgar

Taiwan Journal of Ophthalmology, Journal Year: 2024, Volume and Issue: 15(1), P. 149 - 150

Published: Oct. 29, 2024

Language: Английский

Citations

0
Oral cell lysates reduce osteoclastogenesis in murine bone marrow cultures DOI Creative Commons
Layla Panahipour,

Azarakhsh Oladzad Abbasabadi,

Feng Shao

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 19, 2023

Abstract Mechanical and thermal cell damage can occur as a consequence of invasive procedures related to drilling, the insertion dental implants, well periodontal treatments. Necrotic cells release content their cytoplasm membrane fragments thereby signaling need for repair, process that includes bone resorption by osteoclasts inflammation. Here we screened lysates from human gingival fibroblasts, HSC2 TR146 oral squamous carcinoma lines, murine IDG-SW3 osteocytic RAW264.7 macrophage lines potential modulate in vitro osteoclastogenesis marrow cultures. We also tested impact necrotic expression inflammatory cues ST2 stromal cells. report here independent or origin, all significantly reduced cultures; indicated osteoclast marker genes cathepsin K tartrate-resistant acid phosphatase, respective histochemical staining multinucleated found greatly pushed CCL2, CCL5, CXCL1, IL1, IL6 These findings suggest reduce but only damaged force produce an environment.

Language: Английский

Citations

0