Long non‑coding RNA SNHG1 promotes autophagy in vascular smooth muscle cells induced by facilitating CLEC7A DOI Creative Commons

Hao-wei Deng,

Weiping Teng, Shaodan Zhou

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 31(1)

Published: Nov. 5, 2024

Long non‑coding RNAs serve a crucial role in autophagy of vascular smooth muscle cells (VSMCs). The present study aimed to investigate the effect small nucleolar RNA host gene 1 (SNHG1) on VSMCs and associated underlying mechanisms. Rapamycin was used induce effects SNHG1 proliferation migration change phenotype were tested following overexpression silencing SNHG1. target predicted validated. SNHG1‑regulated via C‑type lectin domain family 7 member A (CLEC7A) determined by combined CLEC7A. Rapamycin‑induced changed cell from contractile synthetic, with decreased expression α‑smooth actin protein 22a increased osteopontin. Overexpression caused same while opposite observed silencing. promoted VSMCs. CLEC7A identified as direct binding relationship between them confirmed immunoprecipitation pull‑down assays. both during reduced this In conclusion, autophagy. promotes conversion synthetic facilitating expression.

Language: Английский

Effects of Mandibular Advancement Device on Genioglossus of Rabbits in Obstructive Sleep Apnea Through PINK1/Parkin Pathway DOI Open Access

Lishuang Ma,

Yahui Zhu, Zhang Zuo

et al.

Journal of Oral Rehabilitation, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

ABSTRACT Background Early treatment of mandibular advancement device (MAD) reverses the abnormal changes resulting from obstructive sleep apnoea (OSA), but underlying mechanism is not clear. We analysed genioglossus function before and after MAD in OSA rabbits explored mitochondrial autophagy. Methods Eighteen male New Zealand were randomised into three groups: control group, Group OSA, MAD. After successful modelling, all animals induced supine positions for 4–6 h per day 8 weeks. Cone beam computed tomography (CBCT) polysomnography (PSG) performed to record conditions. The contractile force levels LC3‐I, LC3‐II, Beclin‐1, PINK1 Parkin detected groups. In vitro, C2C12 myoblast cells cultured under normoxic or hypoxic conditions 24 h, then structure accumulation autolysosomes by transmission electron microscopy (TEM). Results tension was significantly lower than that group. ratio LC3II/LC3I higher And tended be normal treatment. disrupted, number increased hypoxia. Conclusions may decrease increase level autophagy caused OSA. mediated PINK1/Parkin pathway rabbits.

Language: Английский

Citations

1
Epigenetic targeting of autophagy for cancer: DNA and RNA methylation DOI Creative Commons

Luobin Lin,

Yun‐Tao Zhao, Qinzhou Zheng

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: Dec. 8, 2023

Autophagy, a crucial cellular mechanism responsible for degradation and recycling of intracellular components, is modulated by an intricate network molecular signals. Its paradoxical involvement in oncogenesis, acting as both tumor suppressor promoter, has been underscored recent studies. Central to this regulatory are the epigenetic modifications DNA RNA methylation, notably presence N6-methyldeoxyadenosine (6mA) genomic N6-methyladenosine (m6A) eukaryotic mRNA. The 6mA modification adds extra dimension regulation, potentially impacting transcriptional dynamics genes linked autophagy and, especially, cancer. Conversely, m6A modification, governed methyltransferases demethylases, influences mRNA stability, processing, translation, affecting central autophagic pathways. As we delve deeper into complexities importance these methylation grows more evident. interplay 6mA, m6A, points layered mechanism, illuminating reactions range conditions. This review delves nexus between their influence on cancer contexts. By closely examining markers, underscore promise therapeutic avenues, suggesting novel approaches intervention through modulation.

Language: Английский

Citations

3
Physiological and Pathogenesis Significance of Chorein in Health and Disease DOI Creative Commons

Saad Alkahtani,

AA Alkahtane,

Saud Alarifi

et al.

Physiological Research, Journal Year: 2024, Volume and Issue: unknown, P. 189 - 203

Published: April 30, 2024

This comprehensive review explores the physiological and pathophysiological significance of VPS13A, a protein encoded by VPS13A gene. The gene is associated with Chorea-acanthocytosis (ChAc), rare hereditary neurodegenerative disorder. covers essential aspects, beginning genetics highlighting its role in pathogenesis ChAc, addressing spectrum genetic variants involved. It delves into structure function protein, emphasizing presence various tissues potential involvement trafficking lipid homeostasis. Molecular functions brain tissue other cell types or respect to their cytoskeletal regulation autophagy are explored. Finally, it intriguing link between mutations, imbalances, neurodegeneration, shedding light on future research directions. Overall, this serves as resource for understanding pivotal health disease, particularly context ChAc. Key words: Chorein , Tumor, Actin, Microfilament, Gene expression, Chorea-acanthocytosis.

Language: Английский

Citations

0
Heat stress promotes osteogenic and odontogenic differentiation of stem cells from apical papilla via glucose-regulated protein 78-mediated autophagy DOI Creative Commons
Xiaolan Zhang, Wei Zhou,

Yunlong Xu

et al.

Journal of Dental Sciences, Journal Year: 2024, Volume and Issue: 20(1), P. 487 - 501

Published: May 15, 2024

Heat stress is essential for improving the efficacy of mesenchymal stem cell (MSC)-based regeneration medicine. However, it still unclear whether and how heat influences differentiation cells from apical papilla (SCAPs). This research aimed to explore potential mechanism glucose-regulated protein 78 (GRP78) in regulating under SCAPs. The proliferation ability was assessed using 5-Ethynyl-2'- deoxyuridine (EdU) assay, counting kit assay (CCK-8), flow cytometry (FCM). osteogenic odontogenic capacities were investigated through Western blot, quantitative reverse transcription polymerase chain reaction (qRT-PCR), alkaline phosphatase (ALP) staining activity alizarin red S (ARS) staining, as well immunofluorescence staining. blot transmission electron microscopy (TEM) used detect autophagy. enhanced SCAPs, but did not significantly affect proliferation. Besides, GRP78 has been confirmed modulate induced by stress. Autophagy triggered while knockdown or inhibitor autophagy suppressed differentiation. induces SCAPs GRP78-mediated

Language: Английский

Citations

0
Long non‑coding RNA SNHG1 promotes autophagy in vascular smooth muscle cells induced by facilitating CLEC7A DOI Creative Commons

Hao-wei Deng,

Weiping Teng, Shaodan Zhou

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 31(1)

Published: Nov. 5, 2024

Long non‑coding RNAs serve a crucial role in autophagy of vascular smooth muscle cells (VSMCs). The present study aimed to investigate the effect small nucleolar RNA host gene 1 (SNHG1) on VSMCs and associated underlying mechanisms. Rapamycin was used induce effects SNHG1 proliferation migration change phenotype were tested following overexpression silencing SNHG1. target predicted validated. SNHG1‑regulated via C‑type lectin domain family 7 member A (CLEC7A) determined by combined CLEC7A. Rapamycin‑induced changed cell from contractile synthetic, with decreased expression α‑smooth actin protein 22a increased osteopontin. Overexpression caused same while opposite observed silencing. promoted VSMCs. CLEC7A identified as direct binding relationship between them confirmed immunoprecipitation pull‑down assays. both during reduced this In conclusion, autophagy. promotes conversion synthetic facilitating expression.

Language: Английский

Citations

0