Published: Jan. 1, 2023
The application of digital holographic microscopy (DHM) in biomedical laboratories requires a fast and robust image acquisition. In overview, different quantitative DHM phase contrast approaches towards high-throughput analysis adherent suspended cells are discussed.
Language: Английский
Sensors, Journal Year: 2024, Volume and Issue: 24(6), P. 1950 - 1950
Published: March 19, 2024
Digital Holographic Microscopy (DHM) is a 3D imaging technology widely applied in biology, microelectronics, and medical research. However, the noise generated during process can affect accuracy of diagnoses. To solve this problem, we proposed several frequency domain filtering algorithms. algorithms have limitation that they only be when distance between direct current (DC) spectrum sidebands are sufficiently far. address these limitations, among algorithms, HiVA algorithm deep learning algorithm, which effectively filter by distinguishing detailed information object, used to enable regardless DC sidebands. In paper, combination traditional image processing methods proposed, aiming reduce profile using Improved Denoising Diffusion Probabilistic Models (IDDPM) algorithm.
Language: Английский
Citations
10
Communications Medicine, Journal Year: 2023, Volume and Issue: 3(1)
Published: Nov. 7, 2023
The clinical spectrum of acute SARS-CoV-2 infection ranges from an asymptomatic to life-threatening disease. Considering the broad severity, reliable biomarkers are required for early risk stratification and prediction outcomes. Despite numerous efforts, no COVID-19-specific biomarker has been established guide further diagnostic or even therapeutic approaches, most likely due insufficient validation, methodical complexity, economic factors. COVID-19-associated coagulopathy is a hallmark disease mainly attributed dysregulated immunothrombosis. This process describes intricate interplay platelets, innate immune cells, coagulation cascade, vascular endothelium leading both micro- macrothrombotic complications. In this context, increased levels immunothrombotic components, including platelet platelet-leukocyte aggregates, have described linked COVID-19 severity.Here, we describe label-free quantitative phase imaging approach, allowing identification cell-aggregates their components at single-cell resolution within 30 min, which prospectively qualifies method as point-of-care (POC) testing.We find significant association between severity amount aggregates. Additionally, observe linkage aggregate composition, size distribution platelets in aggregates.This study presents POC-compatible rapid analysis blood cell aggregates patients with COVID-19.The human body produces series responses when it gets infected SARS-CoV-2, virus that causes COVID-19. One these involves clotting factor sticking cells form bloodstream. We aimed understand significance progression. A approach was used investigate number patient blood. observed severe associated higher numbers specific composition Our can potentially support prevent complications other medical disorders, where shown aggregate.
Language: Английский
Citations
12
Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown
Published: March 27, 2025
ABSTRACT The Clinical Microbiology Open (CMO) is a meeting sponsored by the American Society for (ASM) in collaboration with its Corporate Council and Public Health representatives, which held to discuss topics that are relevant both industry practicing clinical microbiologists. 2024 CMO was Oceanside, California on February 1 2. Participants included public health laboratory directors, representatives from government agencies, biotechnology partners. group engaged discussions theme, “The Lab of Future.” One primary discussed artificial intelligence (AI) opportunities microbiology laboratories. This report summarizes discussion sentiment regarding AI tools, challenges laboratories, potential future directions practice.
Language: Английский
Citations
0
Sensors, Journal Year: 2025, Volume and Issue: 25(9), P. 2675 - 2675
Published: April 23, 2025
We present a single-capture multimodal bright-field (BF) and quantitative phase imaging (QPI) approach that enables the analysis of large, connected, or extended samples, such as confluent cell layers tissue sections. The proposed concept integrates fiber-optic Mach–Zehnder interferometer-based off-axis digital holographic microscopy (DHM) with an inverted commercial optical BF microscope. Utilizing 8-bit grayscale dynamic range multiplexing, we simultaneously capture both images holograms, which are then demultiplexed numerically via Fourier filtering, aberration compensation, weighted image subtraction procedures. Compared to previous BF-DHM systems, our system avoids synchronization challenges caused by multiple recording devices, improves acquisition speed, enhances versatility for fast rapidly moving samples. Initially, perform systematic characterization system’s performance optimizing numerical well coherent incoherent illumination parameters. Subsequently, application capabilities evaluated living cells. results highlight potential biomedical imaging.
Language: Английский
Citations
0
Optics and Lasers in Engineering, Journal Year: 2024, Volume and Issue: 184, P. 108580 - 108580
Published: Sept. 16, 2024
Language: Английский
Citations
3
Photonics, Journal Year: 2023, Volume and Issue: 10(7), P. 817 - 817
Published: July 13, 2023
Inspiring development in optical imaging enables great applications the science and engineering industry, especially medical area. Photonic time-stretch is one emerging innovation that attracted a wide range of attention due to its principle one-to-one-to-one mapping among space-wavelength-time using dispersive medium both spatial time domains. The ultrafast speed photonics technique achieves an ultrahigh frame rate tens millions frames per second, which exceeds traditional methods several orders magnitudes. Additionally, regarding signal processing, it can combine other technologies, such as compressive sensing, nonlinear deep learning. In this paper, we review recent photonic discuss future trends.
Language: Английский
Citations
5
Optics Communications, Journal Year: 2024, Volume and Issue: 561, P. 130522 - 130522
Published: March 27, 2024
Language: Английский
Citations
1
Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S2)
Published: July 18, 2024
SignificanceQuantitative phase imaging (QPI) techniques offer intrinsic information about the sample of interest in a label-free, noninvasive manner and have an enormous potential for wide biomedical applications with negligible perturbations to natural state vitro.AimWe aim present in-depth review scattering formulation light–matter interactions as applied biological samples such cells tissues, discuss relevant quantitative measurement techniques, summary various reported applications.ApproachWe start theory properties followed by exploration microscopy configurations 2D QPI structure dynamics.ResultsWe reviewed 157 publications presented range discussed suitable each. We also theoretical frameworks reconstruction associated highlighted their domains validity.ConclusionsWe provide detailed well system-level techniques. Our study can serve guideline new researchers looking exhaustive literature methods applications.
Language: Английский
Citations
1
Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S2)
Published: Aug. 19, 2024
SignificanceDigital holographic microscopy (DHM) is a label-free technique that provides time-resolved quantitative phase imaging (QPI) by measuring the optical path delay of light induced transparent biological samples. DHM has been utilized for various biomedical applications, such as cancer research and sperm cell assessment, well in vitro drug or toxicity testing. Its lensless version, digital (DLHM), an emerging technology offers size-reduced, lightweight, cost-effective systems. These features make DLHM applicable, example, limited resource laboratories, remote areas, point-of-care applications.AimIn addition to abovementioned advantages, in-line arrangements also include limitation twin-image presence, which can restrict accurate QPI. We therefore propose compact common-path interferometric off-axis approach capable fast-moving specimens, living cells flow.ApproachWe suggest spatially multiplexed (LESSMIM) lens-free variant previously reported (SMIM) concept. LESSMIM comprises architecture based on single diffraction grating achieve holography. From series single-shot holograms, free QPI achieved commonly used methods Fourier filtering-based reconstruction, aberration compensation, numerical propagation.ResultsInitially, concept experimentally demonstrated results from resolution test chart investigations temporal stability. Then, accuracy capabilities adherent cultures characterized. Finally, utilizing microfluidic channel, cytometry suspended flow evaluated.ConclusionsLESSMIM overcomes several limitations fast arrangement. In summary, represents promising with potential applications analysis.
Language: Английский
Citations
1
Biomedical Optics Express, Journal Year: 2023, Volume and Issue: 15(4), P. 2202 - 2202
Published: Dec. 18, 2023
Probiotic bacteria are widely used in pharmaceutics to offer health benefits. Microencapsulation is deliver probiotics into the human body. Capsules stomach have keep constrained until release occurs intestine. Once outside, must maintain enough motility reach intestine walls. Here, we develop a platform based on two label-free optical modules for rapidly screening and ranking probiotic candidates laboratory. Bio-speckle dynamics assay tests microencapsulation effectiveness by simulating gastrointestinal transit. Then, digital holographic microscope 3D-tracks their profiles at single element level rank strains.
Language: Английский
Citations
3