Integrated multi-omics characterization of SMAD4 mutant colorectal cancer DOI Creative Commons

Danyi Zhao,

Yanjun Qu,

Na Gao

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 29, 2024

Colorectal cancer is one of the most common cancers around world, which a severe threat to people's health. SMAD4 belongs dwarfin/SMAD family, plays crucial role in TGF-β and BMP signal pathways. As molecular characterization colon patients following mutations remains unclear, we integrated multi-omics data mutant reveal profile mutation. A missense mutation type SMAD4. Patients with had worse survival. Tumor tissues from carrying showed reduction various immune cells, such as CD4 + memory T cells B cells. Many differential genes were identified compared mutation-free group could be significantly enriched for tumor- immune-related signaling In addition, different drug sensitivities than non-mutant group.

Language: Английский

Elevated BEAN1 expression correlates with poor prognosis, immune evasion, and chemotherapy resistance in rectal adenocarcinoma DOI Creative Commons

Tiannake Shapaer,

Yi Chen,

Yipeng Pan

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 14, 2024

Language: Английский

Citations

15
Integrating spatial and single-cell transcriptomics reveals tumor heterogeneity and intercellular networks in colorectal cancer DOI Creative Commons
Jing Xiao, Xinyang Yu,

Fanlin Meng

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(5)

Published: May 10, 2024

Abstract Single cell RNA sequencing (scRNA-seq), a powerful tool for studying the tumor microenvironment (TME), does not preserve/provide spatial information on tissue morphology and cellular interactions. To understand crosstalk between diverse components in proximity TME, we performed scRNA-seq coupled with transcriptomic (ST) assay to profile 41,700 cells from three colorectal cancer (CRC) tumor-normal-blood pairs. Standalone analyses revealed eight major populations, including B cells, T Monocytes, NK Epithelial Fibroblasts, Mast Endothelial cells. After identification of malignant epithelial observed seven subtypes that reflect heterogeneous status tumor, tumor_CAV1, tumor_ATF3_JUN | FOS, tumor_ZEB2, tumor_VIM, tumor_WSB1, tumor_LXN, tumor_PGM1. By transferring annotations obtained by ST spots, annotated four regions cryosection CRC patients, stroma, immune infiltration, colon epithelium regions. Furthermore, intensive intercellular interactions stroma which were extremely proximal cryosection. In particular, one pair ligands receptors (C5AR1 RPS19) was inferred play key roles For region, typical feature TMSB4X -high expression identified, could be potential marker CRC. The region found characterized VIM expression, suggesting it fostered stromal niche TME. Collectively, single analysis our study reveal heterogeneity molecular provides insights into mechanisms underlying progression may contribute development anticancer therapies targeting non-tumor components, such as extracellular matrix (ECM) genes identified facilitate new

Language: Английский

Citations

14
Targeting the devil: Strategies against cancer-associated fibroblasts in colorectal cancer DOI
Yuting Chen, Zhiyong Liang, Maode Lai

et al.

Translational research, Journal Year: 2024, Volume and Issue: 270, P. 81 - 93

Published: April 16, 2024

Language: Английский

Citations

13
Crosstalk of pyroptosis and cytokine in the tumor microenvironment: from mechanisms to clinical implication DOI Creative Commons
Hua Wang, Tao Wang, Shuxiang Yan

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Nov. 30, 2024

In the realm of cancer research, tumor microenvironment (TME) plays a crucial role in initiation and progression, shaped by complex interactions between cells surrounding non-cancerous cells. Cytokines, as essential immunomodulatory agents, are secreted various cellular constituents within TME, including immune cells, cancer-associated fibroblasts, themselves. These cytokines facilitate intricate communication networks that significantly influence initiation, metastasis, suppression. Pyroptosis contributes to TME remodeling promoting release pro-inflammatory sustaining chronic inflammation, impacting processes such escape angiogenesis. However, challenges remain due interplay among cytokines, pyroptosis, along with dual effects pyroptosis on progression therapy-related complications like cytokine syndrome. Unraveling these complexities could strategies balance inflammatory responses while minimizing tissue damage during therapy. This review delves into crosstalk elucidating their contribution metastasis. By synthesizing emerging therapeutic targets innovative technologies concerning this aims provide novel insights enhance treatment outcomes for patients.

Language: Английский

Citations

9
Integrated analysis of non‑coding RNAs (HOTAIR and miR‑130a) and their cross‑talk with TGF‑β1, SIRT1 and E‑cadherin as potential biomarkers in colorectal cancer DOI Open Access
Ghada Ayeldeen, Bahaa Badr, Olfat Shaker

et al.

Oncology Letters, Journal Year: 2025, Volume and Issue: 29(3)

Published: Jan. 3, 2025

Molecular changes have a substantial impact on the onset of colorectal cancer (CRC). Complexes HOTAIR and miRNAs disrupt several cellular functions during carcinogenesis, primarily by disrupting carcinogenic signaling pathways. In present study, relationships between serum levels transforming growth factor-β1 (TGF-β1), sirtuin-1 (SIRT1) E-cadherin those HOX transcript antisense intergenic RNA (HOTAIR) microRNA-130a (miR-130a) in individuals with CRC were analyzed, including their correlations diagnostic potential. Patients colon healthy volunteers enrolled study. Blood samples collected from 70 patients 30 age-matched control reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine miR-130a. addition, TGF-β1, SIRT1 determined utilizing enzyme-linked immunosorbent assays. found significantly higher SIRT1, miR-130a than participants. high-grade had compared low-grade CRC. A significant reduction observed participants participants, but no difference detected according grade Positive miR-130a, as well TGF-β1 SIRT1. By contrast, negative noted HOTAIR, Therefore, it may be concluded that miR-130a/HOTAIR TGF-β1/SIRT1/E-cadherin axes serve novel biomarkers for early diagnosis

Language: Английский

Citations

1
Global trends in tumor microenvironment-related research on tumor vaccine: a review and bibliometric analysis DOI Creative Commons
Ying Liu,

Sixin Li,

Lu Chen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 6, 2024

Tumor vaccines have become crucial in cancer immunotherapy, but, only a limited number of phase III clinical trials demonstrated efficacy. The crux this issue is the inability tumor to effectively harmonize microenvironment with its intricate interplay. One factor that can hinder effectiveness natural immunosuppressive element present microenvironment. This lead low rates T-cell response specific antigens and development acquired resistance. Conversely, anticancer alter conflicting manners, inducing both immune activation immunological evasion. Hence, comprehending correlation between would establish foundation for forthcoming treatment.

Language: Английский

Citations

7
Probiotic-Derived Metabolites from Lactiplantibacillus plantarum OC01 Reprogram Tumor-Associated Macrophages to an Inflammatory Anti-Tumoral Phenotype: Impact on Colorectal Cancer Cell Proliferation and Migration DOI Creative Commons
Beatrice Garavaglia, Letizia Vallino, Alessandra Ferraresi

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 339 - 339

Published: Feb. 3, 2025

Background: Tumor-associated macrophages (TAMs) are key players in the colorectal cancer (CRC) tumor microenvironment (TME), representing most abundant immune cells within it. The interplay between intestinal microbiota, macrophages, and significantly impacts progression by driving macrophage polarization. Particularly, polarization into pro-tumoral M2-like TAM phenotype promotes extracellular matrix remodeling, cell proliferation, metastasis, suppression, therapy resistance. Probiotic metabolites can disrupt this crosstalk, possibly reverting toward a pro-inflammatory anti-tumoral phenotype, thus potentially benefiting mucosa opposing CRC progression. Previously, we showed that Lactiplantibacillus plantarum OC01 counter interleukin (IL)-6-induced proliferation migration. Methods: Here, explore how probiotics affect secretome influences polarization, which then malignancy. Results: conditioning medium (CM) from indeed promoted of whereas CM pre-treated with L. induced characterized NLRP3 inflammasome activation reactive oxygen species (ROS) production, decreased expression M2 markers CD206 CD163. Consistently, growth factor (TGF)-β, promoter was reduced treated OC01. inhibited Conclusions: Overall, our study highlights potential to reprogram metabolism reshape TME shifting TAMs more inflammatory emphasizing promise advancing novel therapeutic approaches for CRC.

Language: Английский

Citations

0
Exploring Mechanisms of Immunosuppression in Colorectal Cancer: Interaction of MDSCs with TGF-<i>β</i>1 DOI

鹏远 陈

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(02), P. 1864 - 1878

Published: Jan. 1, 2025

Language: Английский

Citations

0
Identification of lncRNA associated with the SERPINE1 gene in colorectal cancer through TGF-β pathway DOI

Ghazale Habibzadeh,

Khatere Mokhtari,

Masoumeh Heshmati

et al.

Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 190, P. 110037 - 110037

Published: March 19, 2025

Language: Английский

Citations

0
Characterizing macrophage diversity in colorectal malignancies through single-cell genomics DOI Creative Commons
Tianjing Zhao,

Yinyi Luo,

Yuanjie Sun

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Colorectal cancer (CRC) is one of the most common malignant tumors digestive tract, with increasing incidence and mortality rates, posing a significant burden on human health. Its progression relies various mechanisms, among which tumor microenvironment tumor-associated macrophages (TAMs) have garnered attention. Macrophage infiltration in solid associated poor prognosis linked to chemotherapy resistance many cancers. These biological behaviors depend heterogeneity macrophages. Tumor-promoting TAMs comprise subpopulations characterized by distinct markers unique transcriptional profiles, rendering them potential targets for anticancer therapies through either depletion or reprogramming from pro-tumoral an anti-tumoral state. Single-cell RNA sequencing technology has significantly enhanced our research resolution, breaking traditional simplistic definitions macrophage subtypes deepening understanding diversity within TAMs. However, unified elucidation nomenclature molecular characteristics this remains lacking. In review, we assess application conventional polarization colorectal malignancies explore several defined single-cell omics perspective recent years, categorizing based their functions.

Language: Английский

Citations

0