Exploring Bioelectricity with Ace2N-mNeon during Zebrafish Embryogenesis DOI Creative Commons

Zhenyang Wu,

Renata Silva,

Ruya Houssein

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Bioelectricity is a fundamental biophysical phenomenon present in all cells, playing crucial role embryogenesis by regulating processes such as neuronal signaling, pattern formation, and cancer suppression. Precise monitoring of bioelectric signals their dynamic changes throughout development vital for advancing our understanding higher organisms. However, the lack suitable techniques mapping during early has greatly limited ability to interpret these mechanisms. To address this challenge, we developed an Ace2N-mNeon expression library zebrafish, which exhibits membrane localization from 4 hours post-fertilization at least 5 days post- fertilization broad across multiple cell types development. We validated use studying via voltage imaging record neurons cardiomyocytes different stages. Through approach, found evidence synchronized activity observed faster dynamics progressed. Our results show that valuable tool developmental studies supporting advanced fluorescence lifetime (FLIM). These methods enable non-invasive, diverse development, significantly surpassing capabilities current electrophysiological techniques.

Language: Английский

Bioelectricity is a universal multifaced signaling cue in living organisms DOI
Guangjun Zhang, Michael Levin

Molecular Biology of the Cell, Journal Year: 2025, Volume and Issue: 36(2)

Published: Jan. 28, 2025

The cellular electrical signals of living organisms were discovered more than a century ago and have been extensively investigated in the neuromuscular system. Neuronal depolarization hyperpolarization are essential for our physiological pathological functions. Bioelectricity is being recognized as an ancient, intrinsic, fundamental property all cells, it not limited to Instead, emerging evidence supports view bioelectricity instructional signaling cue physiology, embryonic development, regeneration, human diseases, including cancers. Here, we highlight current understanding share views on challenges perspectives.

Language: Английский

Citations

2
Developmental Bioelectricity as an Explanatory Framework for Cognition and Meaning DOI Creative Commons
Ahti‐Veikko Pietarinen, Majid Davoody Beni, Vera Shumilina

et al.

Biosemiotics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

1
Wireless control of nerve growth using bipolar electrodes: a new paradigm in electrostimulation DOI Creative Commons
Ann M. Rajnicek, N. Casañ-Pastor

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(9), P. 2180 - 2202

Published: Jan. 1, 2024

Electrical activity underpins all life, but is most familiar in the nervous system, where long range electrical signalling essential for function. When this lost (

Language: Английский

Citations

8
Bioelectricity in dental medicine: a narrative review DOI Creative Commons
Qingqing Min, Yajun Gao, Yao Wang

et al.

BioMedical Engineering OnLine, Journal Year: 2024, Volume and Issue: 23(1)

Published: Jan. 3, 2024

Abstract Background Bioelectric signals, whether exogenous or endogenous, play crucial roles in the life processes of organisms. Recently, significance bioelectricity field dentistry is steadily gaining greater attention. Objective This narrative review aims to comprehensively outline theory, physiological effects, and practical applications dental medicine offer insights into its potential future direction. It attempts provide clinicians researchers with an electrophysiological perspective enhance their clinical practice fundamental research endeavors. Methods An online computer search for relevant literature was performed PubMed, Web Science Cochrane Library, keywords “bioelectricity, endogenous electric signal, stimulation, medicine.” Results Eventually, 288 documents were included review. The variance ion concentration between interior exterior cell membrane, referred as transmembrane potential, forms basis bioelectricity. Transmembrane has been established essential regulator intercellular communication, mechanotransduction, migration, proliferation, immune responses. Thus, stimulation can significantly alter cellular action by affecting potential. In medicine, proven useful assessing pulp condition, locating root apices, improving properties biomaterials, expediting orthodontic tooth movement, facilitating implant osteointegration, addressing maxillofacial malignancies, managing neuromuscular dysfunction. Furthermore, reprogramming bioelectric signals holds promise a means guide organism development intervene disease processes. Besides, high-throughput tools will be imperative identifying channel targets precisely modulating future. Conclusions Bioelectricity found application various concepts but large-scale, standardized, randomized controlled trials are still necessary addition, precise, repeatable predictable measurement modulation methods signal patterns Graphical abstract

Language: Английский

Citations

4
The Role of Bioelectric Signals in Cancer Genome Regulation and Potential Therapeutic Implication: An Overview DOI Open Access

Lawrence John Ajutor,

Fatima Bello Sani,

Elijah Nehemiah Kama

et al.

Journal of Cancer and Tumor International, Journal Year: 2025, Volume and Issue: 15(1), P. 24 - 37

Published: Jan. 16, 2025

Bioelectrical signals which are directed by ion channels and membrane potential (V_mem), play a crucial role in many cellular processes including proliferation differentiation. It has also been known to influence such as gene expression, epigenetics, tumor progression key aspects of cancer development. This study explores the bioelectric signaling oncogenesis, highlighting possible therapeutic implications. An inferential review existing literature was done understand outcomes integrating Tumor-Treating Fields (TTFields) with traditional therapies like chemotherapy immunotherapy. Relevant sources were analyzed gain mechanistic insights from clinical non-clinical studies deduce Dysregulated channel activity abnormal hallmark findings cells. Deviant seen tumors promote oncogene activation suppressor silencing. These changes affect chromatin remodeling through pathways involving calcium signaling, histone modifications, DNA methylation. Therapeutically, targeting channels, potassium sodium-proton exchangers may offer novel strategy disrupt growth. Bioelectric stimulation, using techniques optogenetics, can help reprogram cells induce differentiation or apoptosis. There diagnostic advancements that leverage markers, depolarized potential, for early detection electrophysiological imaging wearable sensors. modulation enhance drug uptake, improve immune responses normalizing microenvironment, enable targeted delivery electroporation. genome regulation significant potential. Further recommended provide essential into harnessing bioelectricity advanced management improved patient outcomes.

Language: Английский

Citations

0
Evolution of two‐pore domain potassium channels and their gene expression in zebrafish embryos DOI Creative Commons
Sung Jun Park, Martin R. Silic,

Peyton L. Staab

et al.

Developmental Dynamics, Journal Year: 2024, Volume and Issue: 253(8), P. 722 - 749

Published: Jan. 25, 2024

Abstract Background The two‐pore domain potassium (K2P) channels are a major type of that maintain the cell membrane potential by conducting passive leak currents independent voltage change. They play prominent roles in multiple physiological processes, including neuromodulation, perception pain, breathing and mood control, response to volatile anesthetics. Mutations K2P have been linked many human diseases, such as neuronal cardiovascular disorders cancers. Significant progress has made understand their protein structures, functions, pharmacological modifiers. However, expression function during embryonic development remain largely unknown. Results We employed zebrafish model identified 23 k2p genes using BLAST search gene cloning. first analyzed vertebrate channel evolution phylogenetic syntenic analyses. Our data revealed six subtypes already evolved invertebrates long before emergence vertebrates. Moreover, number increased, most likely due two whole‐genome duplications. Furthermore, we examined early embryogenesis situ hybridization. Each subgroup's showed similar but distinct domains with some exceptions. Most them were expressed neural tissues consistent known excitability regulation. few temporarily specific or organs, suggesting these may be needed for development. Conclusions developmental analyses shed light on evolutionary history related functions channelopathies.

Language: Английский

Citations

1
From the Microbiome to the Electrome: Implications for the Microbiota–Gut–Brain Axis DOI Open Access
Marwane Bourqqia-Ramzi,

Jesús Mansilla-Guardiola,

David Muñoz-Rodríguez

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6233 - 6233

Published: June 5, 2024

The gut microbiome plays a fundamental role in metabolism, as well the immune and nervous systems. Microbial imbalance (dysbiosis) can contribute to subsequent physical mental pathologies. As such, interest has been growing microbiota–gut–brain brain axis bioelectrical communication that could exist between bacterial cells. aim of this study was investigate profile (electrome) two species characteristic microbiome: Proteobacteria Gram-negative bacillus Escherichia coli (E. coli), Firmicutes Gram-positive coccus Enterococcus faecalis faecalis). We analyzed both strains (i) validate fluorescent probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol, DiBAC4(3), reliable reporter changes membrane potential (Vmem) for bacteria; (ii) assess evolution bioelectric throughout growth strains; (iii) effects neural-type stimuli on Vmem changes: excitatory neurotransmitter glutamate (Glu) inhibitory γ-aminobutyric acid (GABA); (iv) examine impact induced by neurotransmitters growth, viability, cultivability using absorbance, live/dead probes, viable counts, respectively. Our findings reveal distinct profiles each phase. Importantly, induce without affecting or cultivability, suggesting specific response cells cues. These results understanding external stimuli, with implications modulating bioelectricity novel therapeutic target.

Language: Английский

Citations

1
Exploring Bioelectricity with Ace2N-mNeon during Zebrafish Embryogenesis DOI Creative Commons

Zhenyang Wu,

Renata Silva,

Ruya Houssein

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Bioelectricity is a fundamental biophysical phenomenon present in all cells, playing crucial role embryogenesis by regulating processes such as neuronal signaling, pattern formation, and cancer suppression. Precise monitoring of bioelectric signals their dynamic changes throughout development vital for advancing our understanding higher organisms. However, the lack suitable techniques mapping during early has greatly limited ability to interpret these mechanisms. To address this challenge, we developed an Ace2N-mNeon expression library zebrafish, which exhibits membrane localization from 4 hours post-fertilization at least 5 days post- fertilization broad across multiple cell types development. We validated use studying via voltage imaging record neurons cardiomyocytes different stages. Through approach, found evidence synchronized activity observed faster dynamics progressed. Our results show that valuable tool developmental studies supporting advanced fluorescence lifetime (FLIM). These methods enable non-invasive, diverse development, significantly surpassing capabilities current electrophysiological techniques.

Language: Английский

Citations

0