Abnormal centriolar biomarker ratios correlate with unexplained bull artificial insemination subfertility: a pilot study

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Oct. 26, 2023

Abstract The mechanisms underlying male infertility are poorly understood. Most mammalian spermatozoa have two centrioles: the typical barrel-shaped proximal centriole (PC) and atypical fan-like distal (DC) connected to axoneme (Ax). These structures essential for fertility. However, relationship between quality subfertility (reduced fertility) is not well established. Here, we tested hypothesis that assessing sperm can identify cattle subfertility. By comparing from 25 fertile 6 subfertile bulls, all with normal semen analyses, found unexplained lower sire conception rates (pregnancy rate artificial insemination in cattle) correlate abnormal centriolar biomarker distribution. Fluorescence-based Ratiometric Analysis of Sperm Centrioles (FRAC) only four bulls (4/25, 16%) had positive FRAC tests (having one or more mean ratios outside distribution range a group’s high-quality population), whereas (6/6, 100%) ( P = 0.00008). most sensitive was acetylated tubulin, which novel labeling pattern DC Ax. data suggest tubulin bull remains undetected by current methods may provide insight into mechanism

Language: Английский

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Abnormal Centriolar Biomarker Ratios Correlate with Unexplained Bull Artificial Insemination Subfertility – a Pilot Study

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 4, 2023

Abstract The mechanisms underlying male infertility are poorly understood. Most mammalian spermatozoa have two centrioles: the typical barrel-shaped proximal centriole (PC) and atypical fan-like distal (DC) connected to axoneme (Ax). These structures essential for fertility. However, relationship between quality subfertility (reduced fertility) is not well established. Here, we tested hypothesis that assessing sperm can identify cattle subfertility. By comparing from 25 fertile 6 subfertile bulls, all with normal semen analyses, found unexplained lower sire conception rates (pregnancy rate artificial insemination in cattle) corelate abnormal centriolar biomarker distribution. Fluorescence-based Ratiometric Analysis of Sperm Centrioles (FRAC) only four bulls (4/25, 16%) had positive FRAC tests (having one or more mean ratios outside distribution range a group’s high-quality population), whereas (6/6 , 100%) (P=0.00008). most sensitive was Acetylated Tubulin, which novel labeling pattern DC Ax. data suggest Tubulin bull remains undetected by current methods may provide insight into mechanism

Language: Английский

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Формирование центриолей в раннем эмбриональном развитии млекопитающих. Новый взгляд в светепоследних открытий, "Природа"

Priroda, Journal Year: 2023, Volume and Issue: 10, P. 3 - 12

Published: Jan. 1, 2023

Центросома — небольшая клеточная органелла, в которой сосредоточено большое количество регуляторных белков. Будучи также центром организации микротрубочек, центросома представляет собой транспортный узел клетки, из которого с помощью специфических белков-моторов по микротрубочкам осуществляется как центробежный, так и центростремительный транспорт белковых комплексов, целых органелл. Хотя за последние годы достигнут значительный прогресс идентификации сотен центросомальных белков, процесс формирования центросомы еще недостаточно изучен. В настоящей статье изложены результаты исследования процессе раннего эмбрионального развития.

Language: Русский

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