Optical coherence tomography angiography analysis methods: a systematic review and meta-analysis

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 26, 2024

Abstract Optical coherence tomography angiography (OCTA) is widely used for non-invasive retinal vascular imaging, but the OCTA methods to assess perfusion vary. We evaluated different between studies. MEDLINE and Embase were searched from 2014 August 2021. included prospective studies including ≥ 50 participants using in either global or systemic disorders. Risk of bias was assessed National Institute Health quality assessment tool observational cohort cross-sectional Heterogeneity data by Q statistics, Chi-square test, I 2 index. Of 5974 identified, 191 this evaluation. The selected employed seven devices, six macula volume dimensions, four subregions, nine analyses, five vessel layer definitions, totalling 197 distinct assessing over 7000 possible combinations. Meta-analysis performed on 88 reporting density foveal avascular zone area, showing lower patients with diabetes mellitus than healthy controls, high heterogeneity. lowest reported effects strongest superficial capillary plexus assessments. Systematic review revealed massive heterogeneity perfusion, supporting calls standardisation methodology.

Language: Английский

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circZNF532 promotes endothelial-to-mesenchymal transition in diabetic retinopathy by recruiting TAF15 to stabilize PIK3CD

Endocrine Journal, Journal Year: 2024, Volume and Issue: 71(7), P. 675 - 686

Published: Jan. 1, 2024

Endothelial-to-mesenchymal transition (EndMT) is a pivotal event in diabetic retinopathy (DR). This study explored the role of circRNA zinc finger protein 532 (circZNF532) regulating EndMT DR progression. Human retinal microvascular endothelial cells (HRMECs) were exposed to high glucose (HG) induce cell model. Actinomycin D-treated HRMECs used confirm mRNA stability phosphoinositide-3 kinase catalytic subunit δ (PIK3CD). The interaction between TATA-box-binding protein-associated factor 15 (TAF15) and circZNF532/PIK3CD was subsequently analyzed using RNA immunoprecipitation (RIP), pull-down. It found that HG treatment accelerated process, facilitated migration angiogenesis, enhanced PIK3CD p-AKT levels HRMECs, whereas si-circZNF532 transfection neutralized these effects. Further data showed circZNF532 recruited TAF15 stabilize PIK3CD, thus elevating expression. Following rescue experiments suggested overexpression partially negated inhibitory effect silencing on EndMT, migration, angiogenesis HG-treated HRMECs. In conclusion, our results suggest recruits thereby facilitating DR.

Language: Английский

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High-throughput ultrastructural analysis of macular telangiectasia type 2

Frontiers in Ophthalmology, Journal Year: 2024, Volume and Issue: 4

Published: July 30, 2024

Introduction Macular Telangiectasia type 2 (MacTel), is an uncommon form of late-onset, slowly-progressive macular degeneration. Associated with regional Müller glial cell loss in the retina and amino acid serine synthesized by cells, disease functionally confined to a central retinal region – MacTel zone. Methods We have used high-throughput multi-resolution electron microscopy techniques, optimized for analysis, study retinas from two women, mother daughter, aged 79 48 years respectively, suffering MacTel. Results In both eyes, principal observations made were changes specific mitochondrial structure outside within zone all types, exception those pigment epithelium (RPE). The lesion areas, which are hallmark MacTel, extend Bruch’s membrane choriocapillaris, through depths retina, include cells RPE, vascular elements, extensive hypertrophic basement material. Where lost, we identified significant population microglial exclusively Henle fiber layer, appear ensheathe fibers, similar that seen normally cells. Discussion Since synthesize serine, whereas neurons do not, propose deficiency, required normal function, may relate underlie development With occurring retina-wide, question remains as why uniquely susceptible

Language: Английский

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In Vitro Models of Diabetes: Focus on Diabetic Retinopathy

Cells, Journal Year: 2024, Volume and Issue: 13(22), P. 1864 - 1864

Published: Nov. 11, 2024

Diabetic retinopathy is a major eye complication in patients with diabetes mellitus, and it the leading cause of blindness visual impairment world. Chronic hyperglycemia induces endothelial damage consequent vascular lesions, resulting global vasculitis, which affects small vessels retina. These lesions ischemic conditions certain areas retina, increase release pro-angiogenic mediators. In addition to pharmacological interventions for controlling blood glycaemic level, main strategies treating diabetic are intravitreal injections drugs, surgical treatments, vitrectomies. The complexity due its close interactions different cell types (endothelial cells, astrocytes, Müller cells). evaluation efficacy novel mainly performed through

Language: Английский

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The Potential of Mesenchymal Stem/Stromal Cells in Diabetic Wounds and Future Directions for Research and Therapy—Is It Time for Use in Everyday Practice?

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12171 - 12171

Published: Nov. 13, 2024

The treatment of diabetic wounds is impaired by the intricate nature diabetes and its associated complications, necessitating novel strategies. utilization mesenchymal stem/stromal cells (MSCs) as a therapeutic modality for chronic recalcitrant in patients an active area investigation aimed at enhancing potential covering tissue regeneration. threat posed to patient their environment presence foot ulcer (DFU) so significant that any additional approach opens new pathways halt progression local changes, which subsequently lead generalized inflammatory process, offers chance reduce risk amputation or even death. This article explores MSCs treatment, examining mechanisms action, clinical application challenges, future directions research therapy.

Language: Английский

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