Pleiotropic functions of SscA on the asexual spore of the human pathogenic fungus Aspergillus fumigatus DOI Creative Commons
Ye-Eun Son,

Jiwoo Han,

Kyung-Tae ‍Lee

et al.

Mycology&#58 An International Journal on Fungal Biology, Journal Year: 2023, Volume and Issue: 15(2), P. 238 - 254

Published: Dec. 25, 2023

Asexual spores, called conidia, are key reproductive fungal particles that enable survival in harsh environmental conditions or host systems. The conidia can infect humans, animals, and plants to cause various diseases. Transcription factors, including VosA, WetA, SscA, have roles formation long-term Aspergillus nidulans. Herein, we report the pleiotropic functions of SscA human pathogen A. fumigatus. deletion sscA increased despite decreased growth. Absence impaired reduced spore resistance stresses, heat, UV, oxidation. Transcriptomic analyses showed involved mRNA expression cell wall organisation-related genes. Importantly, mutant contained an amount β-glucan chitin compared wild type conidia. In addition, conidial gliotoxin production was strain. Overall, has formation, maturation dormancy mycotoxin

Language: Английский

Comparative studies on physicochemical properties, volatile flavor substances and microbial community of Mianning ham at different altitudes DOI Creative Commons
Qianhui Zeng, Lili Ji, Wei Wang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Background This study explores the impact of varying altitudes on quality characteristics Mianning ham. Methods By utilizing Solid-Phase Microextraction—Gas Chromatography–Mass Spectrometry (SPME-GC-MS) technology and high-throughput sequencing techniques, physicochemical properties, volatile flavor compounds, shifts in microbial communities ham at different were investigated. Results Ham’s water content, aw, pH, malondialdehyde content,and nitrite content high higher, while salt low was higher. 112 compounds identified fermented for 0, 1, 2 years altitude altitude, more abundant than those altitude. The main 1-octene-3-ol, Nonyl aldehyde, Octanal, 15 other compounds. At phylum level, dominant bacteria Firmicutes Proteobacteria, fungus Basidiomycota. Staphylococcus bacterium genus Aspergillus fungus.The correlation analysis microorganisms substances showed that Cobetia promoted formation Benzaldehyde altitudes. In contrast, Kocuria 1-Octanol, Heptanol,1-Butanol, 2-Heptanone, 3-Hydroxy-2-butanone, Hexanal Discussion There obvious differences between two

Language: Английский

Citations

1
The MYB-like protein MylA contributes to conidiogenesis and conidial germination in Aspergillus nidulans DOI Creative Commons
Ye-Eun Son,

He-Jin Cho,

Hee-Soo Park

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 25, 2024

Abstract Myeloblastosis (MYB)-like proteins are a family of highly conserved transcription factors in animals, plants, and fungi involved the regulation mRNA expression genes. In this study, we identified characterized one MYB-like protein model organism Aspergillus nidulans . We screened levels genes encoding containing two MYB repeats conidia found that four including flbD , cicD uncharacterized genes, were high conidia. To investigate roles AN4618 AN10944 deletion mutants for each gene generated. Our results revealed was required fungal development. Therefore, further investigated role named as mylA repeats. Functional studies MylA essential normal growth Phenotypic transcriptomic analyses demonstrated affected stress tolerance, cell wall integrity, long-term viability A. addition, germination rate mutant decreased compared with wild-type Overall, study suggests is critical appropriate development, conidial maturation, dormancy,

Language: Английский

Citations

4
Synthetic Generation of Dynamic Omics Data Demonstrates Aspergillus nidulans BrlA Paradoxical Wall Stress Response DOI Creative Commons
Joseph Zavorskas, Harley Edwards,

Walker Huso

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract We propose a method to generate additional dynamic omics trajectories which could support pathway analysis methods such as enrichment analysis, genetic programming, and machine learning. Using long short-term memory neural networks, we can effectively predict an organism’s response stimulus based on initial dataset with relatively few samples. present both in silico proof of principle, model that simulates viral propagation, vitro case study, tracking the dynamics Aspergillus nidulans’ BrlA transcript antifungal agent micafungin. Our experiment was conducted using highly noisy only 25 replicates. This principle shows this operate biological datasets, often have high variance validation achieved maximum R 2 value approximately 0.95 our noisy, stochastically simulated data. achieves 0.71. As any learning application, will work better more data; however, applications attain acceptable metrics very The experiments also revealed novel paradoxical dose-response effect: transcriptional upregulation by nidulans is highest at intermediate dose 10 ng/mL reduced higher lower concentrations

Language: Английский

Citations

0
The novel spore-specific regulator SscA controls Aspergillus conidiogenesis DOI Creative Commons
Ye-Eun Son, Jae‐Hyuk Yu, Hee-Soo Park

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(5)

Published: Sept. 14, 2023

Filamentous fungi produce myriads of asexual spores, which are the main reproductive particles that act as infectious or allergenic agents. Although serial sporogenesis is coordinated by various genetic regulators, there remain uncharacterized transcription factors in Aspergillus. To understand underlying mechanism spore formation, integrity, and viability, we have performed comparative transcriptomic analyses on three Aspergillus species found a spore-specific factor, SscA. SscA has major role conidial maturation dormancy, germination nidulans. Functional studies indicate coordinates wall amino acid production, secondary metabolism A. nidulans conidia. Furthermore, roles conserved other species. Our findings broad functions conidia will help to conidiogenesis

Language: Английский

Citations

7
Germination strategies of stress-resistant Aspergillus conidia DOI Creative Commons
Maryam Ijadpanahsaravi, Han A. B. Wösten

Current Opinion in Food Science, Journal Year: 2024, Volume and Issue: 57, P. 101169 - 101169

Published: May 3, 2024

Aspergilli are highly relevant for the food industry. They pathogens of crops and spoil at different post-harvest stages. On other hand, aspergilli their products used to produce food. Crops contaminated with stress-resistant conidia, while these same spores start fermentation production ingredients. This review discusses recent insights into stress resistance germination Aspergillus conidia how this knowledge can be in agriculture

Language: Английский

Citations

2
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction DOI Creative Commons

Xingyu Wu,

Tingting Zhang, Ke Zhang

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(2), P. 277 - 284

Published: March 4, 2024

Aspergillus niger is an efficient cell factory for organic acids production, particularly l-malic acid, through genetic manipulation. However, the traditional method of collecting A. spores inoculation labor-intensive and resource-consuming. In our study, we used CRISPR-Cas9 system to replace promoter brlA, a key gene in conidiation, with xylose-inducible xylP acid-producing strain RG0095, generating brlAxylP. When induced xylose submerged liquid culture, brlAxylP exhibited significant upregulation conidiation-related genes. This induction allowed us easily collect abundance (>7.1 × 106/mL) medium. Significantly, conidiation approach preserves substantial potential as foundational cellular platform biosynthesis acids, including but not limited acid. summary, study offers simplified strategy streamline preparation stage reduce labor material costs industrial acid production using species.

Language: Английский

Citations

1
Mutual regulation of novel transcription factors RsrD and RsrE positively modulates the production of raw-starch-degrading enzyme in Penicillium oxalicum DOI
Hao Guo,

Li‐Xiang Mo,

Xuemei Luo

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(8)

Published: Aug. 21, 2024

Filamentous fungi can produce raw-starch-degrading enzyme, however, regulation of production enzyme remains poorly understood thus far. Here, two novel transcription factors regulator D (RsrD) and E (RsrE) were identified to participate in the

Language: Английский

Citations

1
MpDLDH gene is crucial for regulating growth and pigment biosynthesis through acetyl-CoA modulation in Monascus purpureus DOI

Feng Liao,

Zichao Hu,

Ling-Qing Zeng

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105215 - 105215

Published: Oct. 1, 2024

Language: Английский

Citations

1
STRIPAK, a fundamental signaling hub of eukaryotic development DOI
Ulrich Kück, Stefanie Pöggeler

Microbiology and Molecular Biology Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

The striatin-interacting phosphatase and kinase (STRIPAK) complex is involved in the regulation of many developmental processes eukaryotic microorganisms all animals, including humans. STRIPAK a component protein 2A (PP2A), highly conserved serine-threonine composed catalytic subunits (PP2Ac), scaffolding subunit (PP2AA) various substrate-directing B regulatory subunits. In particular, B''' called striatin has evoked major interest over last 20 years. Studies fungal systems have contributed substantially to our current knowledge about composition, assembly, cellular localization, as well its role autophagy morphology development. represents signaling hub with kinases thus integrates upstream downstream information from pathways. A profound understanding STRIPAK's fungi opens gateway multifarious functions carried out by higher eukaryotes, contribution malignant cell growth.

Language: Английский

Citations

1
Coordination of two regulators SscA and VosA in Aspergillus nidulans conidia DOI
Ye-Eun Son, Hee-Soo Park

Fungal Genetics and Biology, Journal Year: 2024, Volume and Issue: 171, P. 103877 - 103877

Published: March 1, 2024

Language: Английский

Citations

0